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Genetic characterization of circulating seasonal Influenza A viruses (2005-2009) revealed introduction of oseltamivir resistant H1N1 strains during 2009 in eastern India.
Infect Genet Evol 2010; 10(8):1188-98IG

Abstract

Influenza surveillance was implemented in Kolkata, eastern India in 2005 to identify the circulating subtypes and characterize their genetic diversity. Throat and nasal swabs were collected from outpatients with influenza-like illness (ILI). Of 2844 ILI cases identified at two referral hospitals during October 2005-September 2009, 309 (10.86%) were positive for Influenza A by real time RT-PCR, of which 110 (35.60%) were subtyped as H1N1 and 199 (64.40%) as H3N2. Comparison of the nucleotide (nt) and amino acid (aa) sequences of the HA1 gene for H1N1 and H3N2 strains showed that a subset of strains precede WHO recommended contemporary strains by 1-2 years. The Kolkata H1N1 strains clustered in Clade II, subgroup 2B with A/Brisbane/59/2007 but were distant from the corresponding vaccine strains (New Caledonia/20/99 and A/Solomon Island/3/06). The 2005-06 and 2007 H3N2 strains (15/17) clustered either A/Brisbane/10/2007-like (n=8) or A/Nepal/921/2006 like (n=7) strains, whereas 2008 strains (8/12) and 2009 strains (4/4) were similar to the 2010-11 vaccine strain A/Perth/16/2009. More aa substitutions were found in HA or NA genes of H3N2 than in H1N1 strains. No mutation conferring neuraminidase resistance was observed in any of the strain during 2005-08, however in 2009, drug resistant marker (H275Y) was present in seasonal H1N1, but not in co-circulating H3N2 strains. This is the first report of genetic characterization of circulating Influenza A strains from India. The results also highlight the importance of continuing Influenza surveillance in developing countries of Asia for monitoring unusual strains with pandemic potential and mutations conferring antiviral resistance.

Authors+Show Affiliations

Division of Virology, National Institute of Cholera and Enteric Diseases, P-33 C.I.T. Road, Scheme XM, Beliaghata, Kolkata, West Bengal, India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

20678590

Citation

Agrawal, Anurodh S., et al. "Genetic Characterization of Circulating Seasonal Influenza a Viruses (2005-2009) Revealed Introduction of Oseltamivir Resistant H1N1 Strains During 2009 in Eastern India." Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, vol. 10, no. 8, 2010, pp. 1188-98.
Agrawal AS, Sarkar M, Ghosh S, et al. Genetic characterization of circulating seasonal Influenza A viruses (2005-2009) revealed introduction of oseltamivir resistant H1N1 strains during 2009 in eastern India. Infect Genet Evol. 2010;10(8):1188-98.
Agrawal, A. S., Sarkar, M., Ghosh, S., Roy, T., Chakrabarti, S., Lal, R., ... Chawla-Sarkar, M. (2010). Genetic characterization of circulating seasonal Influenza A viruses (2005-2009) revealed introduction of oseltamivir resistant H1N1 strains during 2009 in eastern India. Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, 10(8), pp. 1188-98. doi:10.1016/j.meegid.2010.07.019.
Agrawal AS, et al. Genetic Characterization of Circulating Seasonal Influenza a Viruses (2005-2009) Revealed Introduction of Oseltamivir Resistant H1N1 Strains During 2009 in Eastern India. Infect Genet Evol. 2010;10(8):1188-98. PubMed PMID: 20678590.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic characterization of circulating seasonal Influenza A viruses (2005-2009) revealed introduction of oseltamivir resistant H1N1 strains during 2009 in eastern India. AU - Agrawal,Anurodh S, AU - Sarkar,Mehuli, AU - Ghosh,Swati, AU - Roy,Tapasi, AU - Chakrabarti,Sekhar, AU - Lal,Renu, AU - Mishra,Akhilesh C, AU - Chadha,Mandeep S, AU - Chawla-Sarkar,Mamta, Y1 - 2010/08/01/ PY - 2010/06/09/received PY - 2010/07/24/revised PY - 2010/07/26/accepted PY - 2010/8/4/entrez PY - 2010/8/4/pubmed PY - 2011/3/19/medline SP - 1188 EP - 98 JF - Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases JO - Infect. Genet. Evol. VL - 10 IS - 8 N2 - Influenza surveillance was implemented in Kolkata, eastern India in 2005 to identify the circulating subtypes and characterize their genetic diversity. Throat and nasal swabs were collected from outpatients with influenza-like illness (ILI). Of 2844 ILI cases identified at two referral hospitals during October 2005-September 2009, 309 (10.86%) were positive for Influenza A by real time RT-PCR, of which 110 (35.60%) were subtyped as H1N1 and 199 (64.40%) as H3N2. Comparison of the nucleotide (nt) and amino acid (aa) sequences of the HA1 gene for H1N1 and H3N2 strains showed that a subset of strains precede WHO recommended contemporary strains by 1-2 years. The Kolkata H1N1 strains clustered in Clade II, subgroup 2B with A/Brisbane/59/2007 but were distant from the corresponding vaccine strains (New Caledonia/20/99 and A/Solomon Island/3/06). The 2005-06 and 2007 H3N2 strains (15/17) clustered either A/Brisbane/10/2007-like (n=8) or A/Nepal/921/2006 like (n=7) strains, whereas 2008 strains (8/12) and 2009 strains (4/4) were similar to the 2010-11 vaccine strain A/Perth/16/2009. More aa substitutions were found in HA or NA genes of H3N2 than in H1N1 strains. No mutation conferring neuraminidase resistance was observed in any of the strain during 2005-08, however in 2009, drug resistant marker (H275Y) was present in seasonal H1N1, but not in co-circulating H3N2 strains. This is the first report of genetic characterization of circulating Influenza A strains from India. The results also highlight the importance of continuing Influenza surveillance in developing countries of Asia for monitoring unusual strains with pandemic potential and mutations conferring antiviral resistance. SN - 1567-7257 UR - https://www.unboundmedicine.com/medline/citation/20678590/Genetic_characterization_of_circulating_seasonal_Influenza_A_viruses__2005_2009__revealed_introduction_of_oseltamivir_resistant_H1N1_strains_during_2009_in_eastern_India_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1567-1348(10)00207-8 DB - PRIME DP - Unbound Medicine ER -