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Simultaneous quantification of amphetamines, caffeine and ketamine in urine by hollow fiber liquid phase microextraction combined with gas chromatography-flame ionization detector.
Talanta. 2010 Aug 15; 82(3):969-75.T

Abstract

A method of hollow fiber (HF) liquid phase microextraction (LPME) combined with gas chromatography (GC)-flame ionization detection (FID) was developed for the simultaneous quantification of trace amphetamine (AP), methamphetamine (MA), methylenedioxyamphetamine (MDA), methylenedioxymethamphetamine (MDMA), caffeine and ketamine (KT) in drug abuser urine samples. The factors affecting on the extraction of six target analytes by HF-LPME were investigated and optimized, and the subsequent analytical performance evaluation and real sample analysis were performed by the extraction of six target analytes in sample solution containing 30% NaCl (pH 12.5) for 20 min with extraction temperature of 30 degrees C and stirring rate of 1000 rpm. Under such optimal conditions, the limits of detection (LODs, S/N=3) for the six target analytes were ranged from 8 microg/L (AP, KT) to 82 microg/L (MDA), with the enrichment factors (EFs) of 5-227 folds, and the relative standard deviations (RSDs, n=7) were in the range of 6.9-14.1%. The correlation coefficients of the calibration for the six target analytes over the dynamic linear range were higher than 0.9958. The application feasibility of HF-LPME-GC-FID in illegal drug monitoring was demonstrated by analyzing drug abuser urine samples, and the recoveries of target drugs for the spiked sample ranging from 75.2% to 119.3% indicated an excellent anti-interference capability of the developed method. The proposed method is simple, effective, sensitive and low-cost, and provides a much more accurate and sensitive detection platform over the conventional analytical techniques (such as immunological assay) for drug abuse analysis.

Authors+Show Affiliations

Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan 430072, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20678654

Citation

Xiong, Jun, et al. "Simultaneous Quantification of Amphetamines, Caffeine and Ketamine in Urine By Hollow Fiber Liquid Phase Microextraction Combined With Gas Chromatography-flame Ionization Detector." Talanta, vol. 82, no. 3, 2010, pp. 969-75.
Xiong J, Chen J, He M, et al. Simultaneous quantification of amphetamines, caffeine and ketamine in urine by hollow fiber liquid phase microextraction combined with gas chromatography-flame ionization detector. Talanta. 2010;82(3):969-75.
Xiong, J., Chen, J., He, M., & Hu, B. (2010). Simultaneous quantification of amphetamines, caffeine and ketamine in urine by hollow fiber liquid phase microextraction combined with gas chromatography-flame ionization detector. Talanta, 82(3), 969-75. https://doi.org/10.1016/j.talanta.2010.06.001
Xiong J, et al. Simultaneous Quantification of Amphetamines, Caffeine and Ketamine in Urine By Hollow Fiber Liquid Phase Microextraction Combined With Gas Chromatography-flame Ionization Detector. Talanta. 2010 Aug 15;82(3):969-75. PubMed PMID: 20678654.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous quantification of amphetamines, caffeine and ketamine in urine by hollow fiber liquid phase microextraction combined with gas chromatography-flame ionization detector. AU - Xiong,Jun, AU - Chen,Jie, AU - He,Man, AU - Hu,Bin, Y1 - 2010/06/08/ PY - 2010/01/19/received PY - 2010/05/31/revised PY - 2010/06/01/accepted PY - 2010/8/4/entrez PY - 2010/8/4/pubmed PY - 2010/12/16/medline SP - 969 EP - 75 JF - Talanta JO - Talanta VL - 82 IS - 3 N2 - A method of hollow fiber (HF) liquid phase microextraction (LPME) combined with gas chromatography (GC)-flame ionization detection (FID) was developed for the simultaneous quantification of trace amphetamine (AP), methamphetamine (MA), methylenedioxyamphetamine (MDA), methylenedioxymethamphetamine (MDMA), caffeine and ketamine (KT) in drug abuser urine samples. The factors affecting on the extraction of six target analytes by HF-LPME were investigated and optimized, and the subsequent analytical performance evaluation and real sample analysis were performed by the extraction of six target analytes in sample solution containing 30% NaCl (pH 12.5) for 20 min with extraction temperature of 30 degrees C and stirring rate of 1000 rpm. Under such optimal conditions, the limits of detection (LODs, S/N=3) for the six target analytes were ranged from 8 microg/L (AP, KT) to 82 microg/L (MDA), with the enrichment factors (EFs) of 5-227 folds, and the relative standard deviations (RSDs, n=7) were in the range of 6.9-14.1%. The correlation coefficients of the calibration for the six target analytes over the dynamic linear range were higher than 0.9958. The application feasibility of HF-LPME-GC-FID in illegal drug monitoring was demonstrated by analyzing drug abuser urine samples, and the recoveries of target drugs for the spiked sample ranging from 75.2% to 119.3% indicated an excellent anti-interference capability of the developed method. The proposed method is simple, effective, sensitive and low-cost, and provides a much more accurate and sensitive detection platform over the conventional analytical techniques (such as immunological assay) for drug abuse analysis. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/20678654/Simultaneous_quantification_of_amphetamines_caffeine_and_ketamine_in_urine_by_hollow_fiber_liquid_phase_microextraction_combined_with_gas_chromatography_flame_ionization_detector_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(10)00427-3 DB - PRIME DP - Unbound Medicine ER -