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In vitro protective effects of Withania somnifera (L.) dunal root extract against hydrogen peroxide and β-amyloid(1-42)-induced cytotoxicity in differentiated PC12 cells.
Phytother Res. 2010 Oct; 24(10):1567-74.PR

Abstract

Withania somnifera L. Dunal (Solanaceae), also known as 'ashwagandha' in Sanskrit and as 'Indian ginseng', is used widely in Ayurvedic medicine as a nerve tonic and memory enhancer, with antiaging, antistress, immunomodulatory and antioxidant properties. There is a paucity of data on the potential neuroprotective effects of W. somnifera root, as traditionally used, against H(2)O(2)- and Aβ((1-42))-induced cytotoxicity which are current targets for novel approaches to treat dementia, especially dementia of the Alzheimer's type (AD). In this study, an aqueous extract prepared from the dried roots of W. somnifera was assessed for potential protective effects against H(2)O(2)- and Aβ((1-42))-aggregated fibril cytotoxicity by an MTT assay using a differentiated rat pheochromocytoma PC12 cell line. The results suggest that pretreatments of differentiated PC12 cells with aqueous extracts of W. somnifera root significantly protect differentiated PC12 cells against both H(2)O(2)- and Aβ((1-42))-induced cytotoxicity, in a concentration dependent manner. To investigate the compounds that could explain the observed effects, the W. somnifera extract was analysed by liquid chromatography-serial mass spectrometry and numerous withanolide derivatives, including withaferin A, were detected. These results demonstrate the neuroprotective properties of an aqueous extract of W. somnifera root and may provide some explanation for the putative ethnopharmacological uses of W. somnifera for cognitive and other neurodegenerative disorders that are associated with oxidative stress.

Authors+Show Affiliations

Medicinal Plant Research Group, School of Agriculture, Food and Rural Development, Newcastle University, Newcastle upon Tyne NE1 7RU, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20680931

Citation

Kumar, S, et al. "In Vitro Protective Effects of Withania Somnifera (L.) Dunal Root Extract Against Hydrogen Peroxide and Β-amyloid(1-42)-induced Cytotoxicity in Differentiated PC12 Cells." Phytotherapy Research : PTR, vol. 24, no. 10, 2010, pp. 1567-74.
Kumar S, Seal CJ, Howes MJ, et al. In vitro protective effects of Withania somnifera (L.) dunal root extract against hydrogen peroxide and β-amyloid(1-42)-induced cytotoxicity in differentiated PC12 cells. Phytother Res. 2010;24(10):1567-74.
Kumar, S., Seal, C. J., Howes, M. J., Kite, G. C., & Okello, E. J. (2010). In vitro protective effects of Withania somnifera (L.) dunal root extract against hydrogen peroxide and β-amyloid(1-42)-induced cytotoxicity in differentiated PC12 cells. Phytotherapy Research : PTR, 24(10), 1567-74. https://doi.org/10.1002/ptr.3261
Kumar S, et al. In Vitro Protective Effects of Withania Somnifera (L.) Dunal Root Extract Against Hydrogen Peroxide and Β-amyloid(1-42)-induced Cytotoxicity in Differentiated PC12 Cells. Phytother Res. 2010;24(10):1567-74. PubMed PMID: 20680931.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro protective effects of Withania somnifera (L.) dunal root extract against hydrogen peroxide and β-amyloid(1-42)-induced cytotoxicity in differentiated PC12 cells. AU - Kumar,S, AU - Seal,C J, AU - Howes,M J R, AU - Kite,G C, AU - Okello,E J, PY - 2010/8/4/entrez PY - 2010/8/4/pubmed PY - 2011/1/5/medline SP - 1567 EP - 74 JF - Phytotherapy research : PTR JO - Phytother Res VL - 24 IS - 10 N2 - Withania somnifera L. Dunal (Solanaceae), also known as 'ashwagandha' in Sanskrit and as 'Indian ginseng', is used widely in Ayurvedic medicine as a nerve tonic and memory enhancer, with antiaging, antistress, immunomodulatory and antioxidant properties. There is a paucity of data on the potential neuroprotective effects of W. somnifera root, as traditionally used, against H(2)O(2)- and Aβ((1-42))-induced cytotoxicity which are current targets for novel approaches to treat dementia, especially dementia of the Alzheimer's type (AD). In this study, an aqueous extract prepared from the dried roots of W. somnifera was assessed for potential protective effects against H(2)O(2)- and Aβ((1-42))-aggregated fibril cytotoxicity by an MTT assay using a differentiated rat pheochromocytoma PC12 cell line. The results suggest that pretreatments of differentiated PC12 cells with aqueous extracts of W. somnifera root significantly protect differentiated PC12 cells against both H(2)O(2)- and Aβ((1-42))-induced cytotoxicity, in a concentration dependent manner. To investigate the compounds that could explain the observed effects, the W. somnifera extract was analysed by liquid chromatography-serial mass spectrometry and numerous withanolide derivatives, including withaferin A, were detected. These results demonstrate the neuroprotective properties of an aqueous extract of W. somnifera root and may provide some explanation for the putative ethnopharmacological uses of W. somnifera for cognitive and other neurodegenerative disorders that are associated with oxidative stress. SN - 1099-1573 UR - https://www.unboundmedicine.com/medline/citation/20680931/In_vitro_protective_effects_of_Withania_somnifera__L___dunal_root_extract_against_hydrogen_peroxide_and_β_amyloid_1_42__induced_cytotoxicity_in_differentiated_PC12_cells_ L2 - https://doi.org/10.1002/ptr.3261 DB - PRIME DP - Unbound Medicine ER -