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Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey.
Food Chem Toxicol. 2010 Aug-Sep; 48(8-9):2227-38.FC

Abstract

Propolis, an extremely complex resinous material, exhibits valuable pharmacological and biological properties attributed to the presence of polyphenols. In this study, we determined the antioxidant properties of lyophilized aqueous extract of propolis (LAEP) from Erzurum province of Turkey and correlated the values with total levels of polyphenolic compounds. In order to estimate the capacity of LAEP to act as antioxidants, we studied its 1,1-diphenyl-2-picryl-hydrazyl radicals (DPPH()), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radicals (ABTS()(+)), N,N-dimethyl-p-phenylenediamine radicals (DMPD()(+)), superoxide anion radicals (O(2)(-)) and hydrogen peroxide (H(2)O(2)) scavenging activity, total antioxidant activity, ferric ions (Fe(3+)) and cupric ions (Cu(2+)) reducing ability, ferrous ions (Fe(2+)) chelating activity. LAEP inhibited 93.2% lipid peroxidation of a linoleic acid emulsion at 30 microg/mL concentration. On the other hand, BHA, BHT, alpha-tocopherol and trolox displayed 83.3%, 82.1%, 68.1% and 81.3% inhibition of peroxidation at the same concentration, respectively. Quantitative amounts of caffeic acid, ferulic acid, syringic acid, ellagic acid, quercetin, alpha-tocopherol, pyrogallol, p-hydroxybenzoic acid, vanillin, p-coumaric acid, gallic acid and ascorbic acid were detected by high performance liquid chromatography and tandem mass spectrometry (LC-MS/MS). This study will bring an innovation for further studies with regard to the antioxidant properties of LAEP.

Links

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Authors+Show Affiliations

Gülçin I
Faculty of Sciences, Department of Chemistry, TAtatürk University, R-25240-Erzurum, Turkey. igulcin@atauni.edu.tr
Bursal E
No affiliation info available
Sehitoğlu MH
No affiliation info available
Bilsel M
No affiliation info available
Gören AC
No affiliation info available

MeSH

Anti-Infective AgentsAntioxidantsChromatography, High Pressure LiquidFlavonoidsFree Radical ScavengersFree RadicalsLimit of DetectionLipid PeroxidationPhenolsPolyphenolsPropolisSpectrometry, Mass, Electrospray IonizationTandem Mass SpectrometryTurkey

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20685228

Citation

Gülçin, Ilhami, et al. "Polyphenol Contents and Antioxidant Activity of Lyophilized Aqueous Extract of Propolis From Erzurum, Turkey." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 48, no. 8-9, 2010, pp. 2227-38.
Gülçin I, Bursal E, Sehitoğlu MH, et al. Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey. Food Chem Toxicol. 2010;48(8-9):2227-38.
Gülçin, I., Bursal, E., Sehitoğlu, M. H., Bilsel, M., & Gören, A. C. (2010). Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 48(8-9), 2227-38. https://doi.org/10.1016/j.fct.2010.05.053
Gülçin I, et al. Polyphenol Contents and Antioxidant Activity of Lyophilized Aqueous Extract of Propolis From Erzurum, Turkey. Food Chem Toxicol. 2010 Aug-Sep;48(8-9):2227-38. PubMed PMID: 20685228.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey. AU - Gülçin,Ilhami, AU - Bursal,Ercan, AU - Sehitoğlu,M Hilal, AU - Bilsel,Mine, AU - Gören,Ahmet C, Y1 - 2010/05/26/ PY - 2010/03/07/received PY - 2010/05/14/revised PY - 2010/05/18/accepted PY - 2010/8/6/entrez PY - 2010/8/6/pubmed PY - 2010/12/22/medline SP - 2227 EP - 38 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem Toxicol VL - 48 IS - 8-9 N2 - Propolis, an extremely complex resinous material, exhibits valuable pharmacological and biological properties attributed to the presence of polyphenols. In this study, we determined the antioxidant properties of lyophilized aqueous extract of propolis (LAEP) from Erzurum province of Turkey and correlated the values with total levels of polyphenolic compounds. In order to estimate the capacity of LAEP to act as antioxidants, we studied its 1,1-diphenyl-2-picryl-hydrazyl radicals (DPPH()), 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radicals (ABTS()(+)), N,N-dimethyl-p-phenylenediamine radicals (DMPD()(+)), superoxide anion radicals (O(2)(-)) and hydrogen peroxide (H(2)O(2)) scavenging activity, total antioxidant activity, ferric ions (Fe(3+)) and cupric ions (Cu(2+)) reducing ability, ferrous ions (Fe(2+)) chelating activity. LAEP inhibited 93.2% lipid peroxidation of a linoleic acid emulsion at 30 microg/mL concentration. On the other hand, BHA, BHT, alpha-tocopherol and trolox displayed 83.3%, 82.1%, 68.1% and 81.3% inhibition of peroxidation at the same concentration, respectively. Quantitative amounts of caffeic acid, ferulic acid, syringic acid, ellagic acid, quercetin, alpha-tocopherol, pyrogallol, p-hydroxybenzoic acid, vanillin, p-coumaric acid, gallic acid and ascorbic acid were detected by high performance liquid chromatography and tandem mass spectrometry (LC-MS/MS). This study will bring an innovation for further studies with regard to the antioxidant properties of LAEP. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/20685228/Polyphenol_contents_and_antioxidant_activity_of_lyophilized_aqueous_extract_of_propolis_from_Erzurum_Turkey_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(10)00331-5 DB - PRIME DP - Unbound Medicine ER -