Citation
Yang, Yunlong, et al. "Molecular Characterization and RNA Interference of Three Midgut Aminopeptidase N Isozymes From Bacillus Thuringiensis-susceptible and -resistant Strains of Sugarcane Borer, Diatraea Saccharalis." Insect Biochemistry and Molecular Biology, vol. 40, no. 8, 2010, pp. 592-603.
Yang Y, Zhu YC, Ottea J, et al. Molecular characterization and RNA interference of three midgut aminopeptidase N isozymes from Bacillus thuringiensis-susceptible and -resistant strains of sugarcane borer, Diatraea saccharalis. Insect Biochem Mol Biol. 2010;40(8):592-603.
Yang, Y., Zhu, Y. C., Ottea, J., Husseneder, C., Leonard, B. R., Abel, C., & Huang, F. (2010). Molecular characterization and RNA interference of three midgut aminopeptidase N isozymes from Bacillus thuringiensis-susceptible and -resistant strains of sugarcane borer, Diatraea saccharalis. Insect Biochemistry and Molecular Biology, 40(8), 592-603. https://doi.org/10.1016/j.ibmb.2010.05.006
Yang Y, et al. Molecular Characterization and RNA Interference of Three Midgut Aminopeptidase N Isozymes From Bacillus Thuringiensis-susceptible and -resistant Strains of Sugarcane Borer, Diatraea Saccharalis. Insect Biochem Mol Biol. 2010;40(8):592-603. PubMed PMID: 20685334.
TY - JOUR
T1 - Molecular characterization and RNA interference of three midgut aminopeptidase N isozymes from Bacillus thuringiensis-susceptible and -resistant strains of sugarcane borer, Diatraea saccharalis.
AU - Yang,Yunlong,
AU - Zhu,Yu Cheng,
AU - Ottea,James,
AU - Husseneder,Claudia,
AU - Leonard,B Rogers,
AU - Abel,Craig,
AU - Huang,Fangneng,
Y1 - 2010/06/02/
PY - 2010/04/07/received
PY - 2010/05/18/revised
PY - 2010/05/27/accepted
PY - 2010/8/6/entrez
PY - 2010/8/6/pubmed
PY - 2011/1/14/medline
SP - 592
EP - 603
JF - Insect biochemistry and molecular biology
JO - Insect Biochem Mol Biol
VL - 40
IS - 8
N2 - Aminopeptidase N (APN) proteins located at the midgut epithelium of some lepidopteran species have been implicated as receptors for insecticidal proteins from Bacillus thuringiensis. cDNAs of three APN isoforms, DsAPN1, DsAPN2, and DsAPN3, from Cry1Ab-susceptible (Cry1Ab-SS) and -resistant (Cry1Ab-RR) strains of the sugarcane borer, Diatraea saccharalis (F.) (Lepidoptera: Crambidae), were identified and sequenced using reverse transcriptase polymerase chain reaction (RT-PCR) and 5' rapid amplification of cDNA end (5' RACE). The characteristic APN sequence features were derived from deduced amino acid sequences of the cloned cDNAs. cDNA sequences of the three APN genes were identical between the Cry1Ab-SS and -RR strains. However, total APN proteolytic activity and gene expression of the three APNs from Cry1Ab-RR larvae were significantly lower than those of the Cry1Ab-SS strain. RNA interference (RNAi) was employed using an oral droplet feeding technique for the three APNs of the Cry1Ab-SS strain. Down-regulating expressions of the three APN genes by RNAi were corresponding to the reductions in the specific APN activity. In addition, silencing of all three APNs in D. saccharalis in vivo by RNAi resulted in a decrease in Cry1Ab susceptibility. Our results showed that reduction in expression of the three APNs is functionally associated with the Cry1Ab resistance in D. saccharalis.
SN - 1879-0240
UR - https://www.unboundmedicine.com/medline/citation/20685334/Molecular_characterization_and_RNA_interference_of_three_midgut_aminopeptidase_N_isozymes_from_Bacillus_thuringiensis_susceptible_and__resistant_strains_of_sugarcane_borer_Diatraea_saccharalis_
L2 - https://linkinghub.elsevier.com/retrieve/pii/S0965-1748(10)00124-4
DB - PRIME
DP - Unbound Medicine
ER -
