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Molecular, immunohistochemical, and pharmacological evidence of oxytocin's role as inhibitor of carbohydrate but not fat intake.
Endocrinology. 2010 Oct; 151(10):4736-44.E

Abstract

Oxytocin (OT) facilitates feeding termination stemming from high osmolality, stomach distention, and malaise. Recent knockout (KO) studies suggested a crucial function for OT in carbohydrate intake: OT-/- mice had increased preference for carbohydrates, including sucrose, but not fat (Intralipid). In striking contrast, sugar appetite was unaffected in the OT receptor KO mouse; data from wild-type animals have been insufficient. Therefore, we examined the involvement of OT in the regulation of sucrose vs. fat intake in C57BL/6 mice that served as a background KO strain. We exposed mice to a meal of sucrose or Intralipid and determined that the percentage of c-Fos-immunoreactive paraventricular hypothalamic OT neurons was elevated at termination of intake of either of the tastants, but this increase was 2-fold higher in sucrose-fed mice. A 48-h exposure to sucrose compared with Intralipid caused up-regulation of OT mRNA, whereas inherent individual preferences for sucrose vs. fat were not associated with differences in baseline OT expression as established with quantitative PCR. We found that L-368,899, an OT receptor antagonist, increased sugar intake when sucrose was presented alone or concurrently with Intralipid; it had no effect on Intralipid or total calorie consumption. L-368,899 affected Fos immunoreactivity in the paraventricular hypothalamus, arcuate nucleus, amygdala, and nucleus of the solitary tract, areas involved in aversion, satiety, and reward. This pattern serves as neuroanatomical basis of OT's complex role in food intake, including sucrose intake. The current findings expand our knowledge on OT and suggest that it acts as a carbohydrate-specific inhibitor of feeding.

Authors+Show Affiliations

Minnesota Obesity Center, University of Minnesota, Department of Food Science and Nutrition, 1334 Eckles Avenue, Saint Paul, Minnesota 55108, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20685878

Citation

Olszewski, Pawel K., et al. "Molecular, Immunohistochemical, and Pharmacological Evidence of Oxytocin's Role as Inhibitor of Carbohydrate but Not Fat Intake." Endocrinology, vol. 151, no. 10, 2010, pp. 4736-44.
Olszewski PK, Klockars A, Olszewska AM, et al. Molecular, immunohistochemical, and pharmacological evidence of oxytocin's role as inhibitor of carbohydrate but not fat intake. Endocrinology. 2010;151(10):4736-44.
Olszewski, P. K., Klockars, A., Olszewska, A. M., Fredriksson, R., Schiöth, H. B., & Levine, A. S. (2010). Molecular, immunohistochemical, and pharmacological evidence of oxytocin's role as inhibitor of carbohydrate but not fat intake. Endocrinology, 151(10), 4736-44. https://doi.org/10.1210/en.2010-0151
Olszewski PK, et al. Molecular, Immunohistochemical, and Pharmacological Evidence of Oxytocin's Role as Inhibitor of Carbohydrate but Not Fat Intake. Endocrinology. 2010;151(10):4736-44. PubMed PMID: 20685878.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Molecular, immunohistochemical, and pharmacological evidence of oxytocin's role as inhibitor of carbohydrate but not fat intake. AU - Olszewski,Pawel K, AU - Klockars,Anica, AU - Olszewska,Agnieszka M, AU - Fredriksson,Robert, AU - Schiöth,Helgi B, AU - Levine,Allen S, Y1 - 2010/08/04/ PY - 2010/8/6/entrez PY - 2010/8/6/pubmed PY - 2010/11/5/medline SP - 4736 EP - 44 JF - Endocrinology JO - Endocrinology VL - 151 IS - 10 N2 - Oxytocin (OT) facilitates feeding termination stemming from high osmolality, stomach distention, and malaise. Recent knockout (KO) studies suggested a crucial function for OT in carbohydrate intake: OT-/- mice had increased preference for carbohydrates, including sucrose, but not fat (Intralipid). In striking contrast, sugar appetite was unaffected in the OT receptor KO mouse; data from wild-type animals have been insufficient. Therefore, we examined the involvement of OT in the regulation of sucrose vs. fat intake in C57BL/6 mice that served as a background KO strain. We exposed mice to a meal of sucrose or Intralipid and determined that the percentage of c-Fos-immunoreactive paraventricular hypothalamic OT neurons was elevated at termination of intake of either of the tastants, but this increase was 2-fold higher in sucrose-fed mice. A 48-h exposure to sucrose compared with Intralipid caused up-regulation of OT mRNA, whereas inherent individual preferences for sucrose vs. fat were not associated with differences in baseline OT expression as established with quantitative PCR. We found that L-368,899, an OT receptor antagonist, increased sugar intake when sucrose was presented alone or concurrently with Intralipid; it had no effect on Intralipid or total calorie consumption. L-368,899 affected Fos immunoreactivity in the paraventricular hypothalamus, arcuate nucleus, amygdala, and nucleus of the solitary tract, areas involved in aversion, satiety, and reward. This pattern serves as neuroanatomical basis of OT's complex role in food intake, including sucrose intake. The current findings expand our knowledge on OT and suggest that it acts as a carbohydrate-specific inhibitor of feeding. SN - 1945-7170 UR - https://www.unboundmedicine.com/medline/citation/20685878/Molecular_immunohistochemical_and_pharmacological_evidence_of_oxytocin's_role_as_inhibitor_of_carbohydrate_but_not_fat_intake_ L2 - https://academic.oup.com/endo/article-lookup/doi/10.1210/en.2010-0151 DB - PRIME DP - Unbound Medicine ER -