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Genetic and comparative genomics mapping reveals that a powdery mildew resistance gene Ml3D232 originating from wild emmer co-segregates with an NBS-LRR analog in common wheat (Triticum aestivum L.).
Theor Appl Genet. 2010 Nov; 121(8):1613-21.TA

Abstract

Powdery mildew caused by Blumeria graminis f. sp. tritici is one of the most important wheat diseases worldwide and breeding for resistance using diversified disease resistance genes is the most promising approach to prevent outbreaks of powdery mildew. A powdery mildew resistance gene, originating from wild emmer wheat (Triticum turgidum var. dicoccoides) accessions collected from Israel, has been transferred into the hexaploid wheat line 3D232 through crossing and backcrossing. Inoculation results with 21 B. graminis f. sp. tritici races indicated that 3D232 is resistant to all of the powdery mildew isolates tested. Genetic analyses of 3D232 using an F(2) segregating population and F(3) families indicated that a single dominant gene, Ml3D232, confers resistance in the host seedling stage. By applying molecular markers and bulked segregant analysis (BSA), we have identified polymorphic simple sequence repeats (SSR), expressed sequence tags (EST) and derived sequence tagged site (STS) markers to determine that the Ml3D232 is located on chromosome 5BL bin 0.59-0.76. Comparative genetic analyses using mapped EST markers and genome sequences of rice and Brachypodium established co-linearity of the Ml3D232 genomic region with a 1.4 Mb genomic region on Brachypodium distachyon chromosome 4, and a 1.2 Mb contig located on the Oryza sativa chromosome 9. Our comparative approach enabled us to develop new EST-STS markers and to delimit the genomic region carrying Ml3D232 to a 0.8 cM segment that is collinear with a 558 kb region on B. distachyon. Eight EST markers, including an NBS-LRR analog, co-segregated with Ml3D232 to provide a target site for fine genetic mapping, chromosome landing and map-based cloning of the powdery mildew resistance gene. This newly developed common wheat germplasm provides broad-spectrum resistance to powdery mildew and a valuable resource for wheat breeding programs.

Authors+Show Affiliations

State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100193, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20686747

Citation

Zhang, Hongtao, et al. "Genetic and Comparative Genomics Mapping Reveals That a Powdery Mildew Resistance Gene Ml3D232 Originating From Wild Emmer Co-segregates With an NBS-LRR Analog in Common Wheat (Triticum Aestivum L.)." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 121, no. 8, 2010, pp. 1613-21.
Zhang H, Guan H, Li J, et al. Genetic and comparative genomics mapping reveals that a powdery mildew resistance gene Ml3D232 originating from wild emmer co-segregates with an NBS-LRR analog in common wheat (Triticum aestivum L.). Theor Appl Genet. 2010;121(8):1613-21.
Zhang, H., Guan, H., Li, J., Zhu, J., Xie, C., Zhou, Y., Duan, X., Yang, T., Sun, Q., & Liu, Z. (2010). Genetic and comparative genomics mapping reveals that a powdery mildew resistance gene Ml3D232 originating from wild emmer co-segregates with an NBS-LRR analog in common wheat (Triticum aestivum L.). TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 121(8), 1613-21. https://doi.org/10.1007/s00122-010-1414-6
Zhang H, et al. Genetic and Comparative Genomics Mapping Reveals That a Powdery Mildew Resistance Gene Ml3D232 Originating From Wild Emmer Co-segregates With an NBS-LRR Analog in Common Wheat (Triticum Aestivum L.). Theor Appl Genet. 2010;121(8):1613-21. PubMed PMID: 20686747.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic and comparative genomics mapping reveals that a powdery mildew resistance gene Ml3D232 originating from wild emmer co-segregates with an NBS-LRR analog in common wheat (Triticum aestivum L.). AU - Zhang,Hongtao, AU - Guan,Haiying, AU - Li,Jingting, AU - Zhu,Jie, AU - Xie,Chaojie, AU - Zhou,Yilin, AU - Duan,Xiayu, AU - Yang,Tsomin, AU - Sun,Qixin, AU - Liu,Zhiyong, Y1 - 2010/08/05/ PY - 2010/03/27/received PY - 2010/07/19/accepted PY - 2010/8/6/entrez PY - 2010/8/6/pubmed PY - 2011/2/3/medline SP - 1613 EP - 21 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 121 IS - 8 N2 - Powdery mildew caused by Blumeria graminis f. sp. tritici is one of the most important wheat diseases worldwide and breeding for resistance using diversified disease resistance genes is the most promising approach to prevent outbreaks of powdery mildew. A powdery mildew resistance gene, originating from wild emmer wheat (Triticum turgidum var. dicoccoides) accessions collected from Israel, has been transferred into the hexaploid wheat line 3D232 through crossing and backcrossing. Inoculation results with 21 B. graminis f. sp. tritici races indicated that 3D232 is resistant to all of the powdery mildew isolates tested. Genetic analyses of 3D232 using an F(2) segregating population and F(3) families indicated that a single dominant gene, Ml3D232, confers resistance in the host seedling stage. By applying molecular markers and bulked segregant analysis (BSA), we have identified polymorphic simple sequence repeats (SSR), expressed sequence tags (EST) and derived sequence tagged site (STS) markers to determine that the Ml3D232 is located on chromosome 5BL bin 0.59-0.76. Comparative genetic analyses using mapped EST markers and genome sequences of rice and Brachypodium established co-linearity of the Ml3D232 genomic region with a 1.4 Mb genomic region on Brachypodium distachyon chromosome 4, and a 1.2 Mb contig located on the Oryza sativa chromosome 9. Our comparative approach enabled us to develop new EST-STS markers and to delimit the genomic region carrying Ml3D232 to a 0.8 cM segment that is collinear with a 558 kb region on B. distachyon. Eight EST markers, including an NBS-LRR analog, co-segregated with Ml3D232 to provide a target site for fine genetic mapping, chromosome landing and map-based cloning of the powdery mildew resistance gene. This newly developed common wheat germplasm provides broad-spectrum resistance to powdery mildew and a valuable resource for wheat breeding programs. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/20686747/Genetic_and_comparative_genomics_mapping_reveals_that_a_powdery_mildew_resistance_gene_Ml3D232_originating_from_wild_emmer_co_segregates_with_an_NBS_LRR_analog_in_common_wheat__Triticum_aestivum_L___ L2 - https://dx.doi.org/10.1007/s00122-010-1414-6 DB - PRIME DP - Unbound Medicine ER -