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Expression of mRNA for proglucagon and glucagon-like peptide-2 (GLP-2) receptor in the ruminant gastrointestinal tract and the influence of energy intake.
Domest Anim Endocrinol 2010; 39(3):181-93DA

Abstract

Glucagon-like peptide-2 (GLP-2) is a potent trophic gut hormone, yet its function in ruminants is relatively unknown. Experiment 1 was conducted as a pilot study to establish the presence of GLP-2 in ruminants and to ascertain whether it was responsive to increased nutrition, as in non-ruminants. Concentrations of intact GLP-2 in the blood and gut epithelial mRNA expression of proglucagon (GCG) and the GLP-2 receptor (GLP2R) were measured in 4 ruminally, duodenally, and ileally cannulated steers. Steers were fed to meet 0.75 x NE(M) for 21 d, and then increased to 1.75 x NE(M) requirement for another 29 d. Blood samples and ruminal, duodenal, and ileal epithelium biopsies were collected at low intake (Days -6 and -3), acute high intake (Days 1 and 3), and chronic high intake (Days 7 and 29) periods. Experiment 2 investigated the mRNA expression pattern of GCG and GLP2R in epithelial tissue obtained from the forestomachs (rumen, omasum, and abomasum) and intestines (duodenum, jejunum, ileum, and colon) of 18 forage-fed Angus steers (260 kg BW). In Experiments 1 and 2, real-time polymerase chain reaction showed that expression of GCG and GLP2R mRNA was detectable in forestomach tissues, but expression was greater (P < 0.001) in small intestinal and colon tissue. High energy intake tended (P = 0.07) to increase plasma GLP-2 during the acute period and was paralleled by a 78% increase (P = 0.07) in ileal GCG mRNA expression. After this initial adaptation, duodenal GCG mRNA expression increased (P = 0.08) during the chronic high intake period. Duodenal GLP2R mRNA expression was not affected by energy intake, but ileal GLP2R expression was increased after 29 d of high energy intake compared to both the low and acute high intake periods (P = 0.001 and P = 0.01, respectively). These data demonstrate that cattle express GCG and GLP2R mRNA primarily in small intestinal and colon tissues. Increased nutrient intake increases ileal GCG mRNA and plasma GLP-2, suggesting that GLP-2 may play a role in the trophic response of the ruminant gastrointestinal tract to increased feed intake.

Authors+Show Affiliations

Department of Animal and Food Sciences, University of Kentucky, Lexington, KY, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

20688461

Citation

Taylor-Edwards, C C., et al. "Expression of mRNA for Proglucagon and Glucagon-like Peptide-2 (GLP-2) Receptor in the Ruminant Gastrointestinal Tract and the Influence of Energy Intake." Domestic Animal Endocrinology, vol. 39, no. 3, 2010, pp. 181-93.
Taylor-Edwards CC, Burrin DG, Matthews JC, et al. Expression of mRNA for proglucagon and glucagon-like peptide-2 (GLP-2) receptor in the ruminant gastrointestinal tract and the influence of energy intake. Domest Anim Endocrinol. 2010;39(3):181-93.
Taylor-Edwards, C. C., Burrin, D. G., Matthews, J. C., McLeod, K. R., Holst, J. J., & Harmon, D. L. (2010). Expression of mRNA for proglucagon and glucagon-like peptide-2 (GLP-2) receptor in the ruminant gastrointestinal tract and the influence of energy intake. Domestic Animal Endocrinology, 39(3), pp. 181-93. doi:10.1016/j.domaniend.2010.05.002.
Taylor-Edwards CC, et al. Expression of mRNA for Proglucagon and Glucagon-like Peptide-2 (GLP-2) Receptor in the Ruminant Gastrointestinal Tract and the Influence of Energy Intake. Domest Anim Endocrinol. 2010;39(3):181-93. PubMed PMID: 20688461.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of mRNA for proglucagon and glucagon-like peptide-2 (GLP-2) receptor in the ruminant gastrointestinal tract and the influence of energy intake. AU - Taylor-Edwards,C C, AU - Burrin,D G, AU - Matthews,J C, AU - McLeod,K R, AU - Holst,J J, AU - Harmon,D L, Y1 - 2010/06/25/ PY - 2010/03/09/received PY - 2010/05/11/revised PY - 2010/05/19/accepted PY - 2010/8/7/entrez PY - 2010/8/7/pubmed PY - 2010/12/21/medline SP - 181 EP - 93 JF - Domestic animal endocrinology JO - Domest. Anim. Endocrinol. VL - 39 IS - 3 N2 - Glucagon-like peptide-2 (GLP-2) is a potent trophic gut hormone, yet its function in ruminants is relatively unknown. Experiment 1 was conducted as a pilot study to establish the presence of GLP-2 in ruminants and to ascertain whether it was responsive to increased nutrition, as in non-ruminants. Concentrations of intact GLP-2 in the blood and gut epithelial mRNA expression of proglucagon (GCG) and the GLP-2 receptor (GLP2R) were measured in 4 ruminally, duodenally, and ileally cannulated steers. Steers were fed to meet 0.75 x NE(M) for 21 d, and then increased to 1.75 x NE(M) requirement for another 29 d. Blood samples and ruminal, duodenal, and ileal epithelium biopsies were collected at low intake (Days -6 and -3), acute high intake (Days 1 and 3), and chronic high intake (Days 7 and 29) periods. Experiment 2 investigated the mRNA expression pattern of GCG and GLP2R in epithelial tissue obtained from the forestomachs (rumen, omasum, and abomasum) and intestines (duodenum, jejunum, ileum, and colon) of 18 forage-fed Angus steers (260 kg BW). In Experiments 1 and 2, real-time polymerase chain reaction showed that expression of GCG and GLP2R mRNA was detectable in forestomach tissues, but expression was greater (P < 0.001) in small intestinal and colon tissue. High energy intake tended (P = 0.07) to increase plasma GLP-2 during the acute period and was paralleled by a 78% increase (P = 0.07) in ileal GCG mRNA expression. After this initial adaptation, duodenal GCG mRNA expression increased (P = 0.08) during the chronic high intake period. Duodenal GLP2R mRNA expression was not affected by energy intake, but ileal GLP2R expression was increased after 29 d of high energy intake compared to both the low and acute high intake periods (P = 0.001 and P = 0.01, respectively). These data demonstrate that cattle express GCG and GLP2R mRNA primarily in small intestinal and colon tissues. Increased nutrient intake increases ileal GCG mRNA and plasma GLP-2, suggesting that GLP-2 may play a role in the trophic response of the ruminant gastrointestinal tract to increased feed intake. SN - 1879-0054 UR - https://www.unboundmedicine.com/medline/citation/20688461/Expression_of_mRNA_for_proglucagon_and_glucagon_like_peptide_2__GLP_2__receptor_in_the_ruminant_gastrointestinal_tract_and_the_influence_of_energy_intake_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0739-7240(10)00052-4 DB - PRIME DP - Unbound Medicine ER -