Tags

Type your tag names separated by a space and hit enter

Genetic variation of the FADS1 FADS2 gene cluster and n-6 PUFA composition in erythrocyte membranes in the European Prospective Investigation into Cancer and Nutrition-Potsdam study.
Br J Nutr. 2010 Dec; 104(12):1748-59.BJ

Abstract

Delta-5 (D5D) and delta-6 (D6D) desaturases are key enzymes in PUFA metabolism. Several factors (e.g. hyperglycaemia, hypertension, blood lipids, statins and fatty acids in diet and biological tissues) may influence desaturase activity. The goals were to evaluate the associations between variation in genes encoding these desaturases (FADS1 and FADS2) and blood concentrations of n-6 PUFA and estimated D5D and D6D activities (evaluated as product/precursor ratio), and to investigate whether other factors influencing the activity of desaturases modify these associations. A random sample of 2066 participants from the European Prospective Investigation into Cancer and Nutrition-Potsdam study (n 27 548) was utilised in the analyses. Crude and adjusted associations between rs174546 genotypes (reflecting genetic variation in the FADS1 FADS2 gene cluster), n-6 PUFA in erythrocytes and estimated desaturase activities were evaluated using multiple linear regression. Potential effect modification was determined by performing stratified analyses and evaluating interaction terms. We found rs174546 genotypes to be related to linoleic (r² 0·060), γ-linolenic (r² 0·041), eicosadienoic (r² 0·034), arachidonic (r² 0·026), docosatetraenoic acids (r² 0·028), estimated D6D activity (r² 0·052) and particularly strongly to dihomo-γ-linolenic acid (DGLA, r² 0·182) and D5D activity (r² 0·231). We did not observe effect modifications with regard to the estimated D5D activity, DGLA and arachidonic acid (AA) for most of the factors evaluated; however, the genetic effect on D5D activity and DGLA may be modified by the dietary n-6:n-3-ratio (P-values for interaction: 0·008 and 0·002), and the genetic effect on DGLA and AA may be modified by lipid-lowering medication (P-values for interaction: 0·0004 and 0·006). In conclusion, genetic variation in the FADS1 FADS2 gene cluster affects n-6 PUFA profiles in erythrocytes reflecting altered D5D activity.

Authors+Show Affiliations

Technische Universität München, Freising, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20691134

Citation

Zietemann, Vera, et al. "Genetic Variation of the FADS1 FADS2 Gene Cluster and N-6 PUFA Composition in Erythrocyte Membranes in the European Prospective Investigation Into Cancer and Nutrition-Potsdam Study." The British Journal of Nutrition, vol. 104, no. 12, 2010, pp. 1748-59.
Zietemann V, Kröger J, Enzenbach C, et al. Genetic variation of the FADS1 FADS2 gene cluster and n-6 PUFA composition in erythrocyte membranes in the European Prospective Investigation into Cancer and Nutrition-Potsdam study. Br J Nutr. 2010;104(12):1748-59.
Zietemann, V., Kröger, J., Enzenbach, C., Jansen, E., Fritsche, A., Weikert, C., Boeing, H., & Schulze, M. B. (2010). Genetic variation of the FADS1 FADS2 gene cluster and n-6 PUFA composition in erythrocyte membranes in the European Prospective Investigation into Cancer and Nutrition-Potsdam study. The British Journal of Nutrition, 104(12), 1748-59. https://doi.org/10.1017/S0007114510002916
Zietemann V, et al. Genetic Variation of the FADS1 FADS2 Gene Cluster and N-6 PUFA Composition in Erythrocyte Membranes in the European Prospective Investigation Into Cancer and Nutrition-Potsdam Study. Br J Nutr. 2010;104(12):1748-59. PubMed PMID: 20691134.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genetic variation of the FADS1 FADS2 gene cluster and n-6 PUFA composition in erythrocyte membranes in the European Prospective Investigation into Cancer and Nutrition-Potsdam study. AU - Zietemann,Vera, AU - Kröger,Janine, AU - Enzenbach,Cornelia, AU - Jansen,Eugene, AU - Fritsche,Andreas, AU - Weikert,Cornelia, AU - Boeing,Heiner, AU - Schulze,Matthias B, Y1 - 2010/08/09/ PY - 2010/8/10/entrez PY - 2010/8/10/pubmed PY - 2011/1/11/medline SP - 1748 EP - 59 JF - The British journal of nutrition JO - Br. J. Nutr. VL - 104 IS - 12 N2 - Delta-5 (D5D) and delta-6 (D6D) desaturases are key enzymes in PUFA metabolism. Several factors (e.g. hyperglycaemia, hypertension, blood lipids, statins and fatty acids in diet and biological tissues) may influence desaturase activity. The goals were to evaluate the associations between variation in genes encoding these desaturases (FADS1 and FADS2) and blood concentrations of n-6 PUFA and estimated D5D and D6D activities (evaluated as product/precursor ratio), and to investigate whether other factors influencing the activity of desaturases modify these associations. A random sample of 2066 participants from the European Prospective Investigation into Cancer and Nutrition-Potsdam study (n 27 548) was utilised in the analyses. Crude and adjusted associations between rs174546 genotypes (reflecting genetic variation in the FADS1 FADS2 gene cluster), n-6 PUFA in erythrocytes and estimated desaturase activities were evaluated using multiple linear regression. Potential effect modification was determined by performing stratified analyses and evaluating interaction terms. We found rs174546 genotypes to be related to linoleic (r² 0·060), γ-linolenic (r² 0·041), eicosadienoic (r² 0·034), arachidonic (r² 0·026), docosatetraenoic acids (r² 0·028), estimated D6D activity (r² 0·052) and particularly strongly to dihomo-γ-linolenic acid (DGLA, r² 0·182) and D5D activity (r² 0·231). We did not observe effect modifications with regard to the estimated D5D activity, DGLA and arachidonic acid (AA) for most of the factors evaluated; however, the genetic effect on D5D activity and DGLA may be modified by the dietary n-6:n-3-ratio (P-values for interaction: 0·008 and 0·002), and the genetic effect on DGLA and AA may be modified by lipid-lowering medication (P-values for interaction: 0·0004 and 0·006). In conclusion, genetic variation in the FADS1 FADS2 gene cluster affects n-6 PUFA profiles in erythrocytes reflecting altered D5D activity. SN - 1475-2662 UR - https://www.unboundmedicine.com/medline/citation/20691134/Genetic_variation_of_the_FADS1_FADS2_gene_cluster_and_n_6_PUFA_composition_in_erythrocyte_membranes_in_the_European_Prospective_Investigation_into_Cancer_and_Nutrition_Potsdam_study_ L2 - https://www.cambridge.org/core/product/identifier/S0007114510002916/type/journal_article DB - PRIME DP - Unbound Medicine ER -