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Differential expression of proteins in the midgut of Anopheles albimanus infected with Plasmodium berghei.
Insect Biochem Mol Biol. 2010 Oct; 40(10):752-8.IB

Abstract

The main vector for transmission of malaria in Mexico is the Anopheles albimanus mosquito. The midgut of disease-transmitting mosquitoes carries out a variety of functions that are related to blood feeding. We analyzed the midgut of A. albimanus infected with Plasmodium berghei (resistant mosquito) using a proteomic approach to identify putative short peptides that are enriched in the midgut after blood feeding. Mosquito midguts were analyzed by two-dimensional electrophoresis to determine the changes in protein profiles. We identified 21 spot proteins that are differentially expressed in the blood of mosquitoes during the immune challenge. Molecular weight of the spots varied from 13 to 36 kDa, with a broad isoelectric point range of 3.92-8.90. We identified the differentially expressed proteins using mass spectrometry and constructed a proteomic data base of the A. albimanus midgut with diverse functions, some of them proteins with digestive and immunologic functions. Identification of these proteins may have important implications for understanding the blood meal digestion process, as well as developing novel vector control strategies and understanding parasite vector interactions.

Authors+Show Affiliations

Centro de Investigaciones Biológicas del Noroeste (CIBNOR), Mar Bermejo 195, Col. Playa Palo de Santa Rita, La Paz, B.C.S. 23096, Mexico. vserrano04@cibnor.mxNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20692341

Citation

Serrano-Pinto, Vania, et al. "Differential Expression of Proteins in the Midgut of Anopheles Albimanus Infected With Plasmodium Berghei." Insect Biochemistry and Molecular Biology, vol. 40, no. 10, 2010, pp. 752-8.
Serrano-Pinto V, Acosta-Pérez M, Luviano-Bazán D, et al. Differential expression of proteins in the midgut of Anopheles albimanus infected with Plasmodium berghei. Insect Biochem Mol Biol. 2010;40(10):752-8.
Serrano-Pinto, V., Acosta-Pérez, M., Luviano-Bazán, D., Hurtado-Sil, G., Batista, C. V., Martínez-Barnetche, J., & Lánz-Mendoza, H. (2010). Differential expression of proteins in the midgut of Anopheles albimanus infected with Plasmodium berghei. Insect Biochemistry and Molecular Biology, 40(10), 752-8. https://doi.org/10.1016/j.ibmb.2010.07.011
Serrano-Pinto V, et al. Differential Expression of Proteins in the Midgut of Anopheles Albimanus Infected With Plasmodium Berghei. Insect Biochem Mol Biol. 2010;40(10):752-8. PubMed PMID: 20692341.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential expression of proteins in the midgut of Anopheles albimanus infected with Plasmodium berghei. AU - Serrano-Pinto,Vania, AU - Acosta-Pérez,Maribel, AU - Luviano-Bazán,Darwin, AU - Hurtado-Sil,Gerardo, AU - Batista,Cesar V F, AU - Martínez-Barnetche,Jesús, AU - Lánz-Mendoza,Humberto, Y1 - 2010/08/06/ PY - 2009/11/27/received PY - 2010/06/24/revised PY - 2010/07/29/accepted PY - 2010/8/10/entrez PY - 2010/8/10/pubmed PY - 2011/1/6/medline SP - 752 EP - 8 JF - Insect biochemistry and molecular biology JO - Insect Biochem. Mol. Biol. VL - 40 IS - 10 N2 - The main vector for transmission of malaria in Mexico is the Anopheles albimanus mosquito. The midgut of disease-transmitting mosquitoes carries out a variety of functions that are related to blood feeding. We analyzed the midgut of A. albimanus infected with Plasmodium berghei (resistant mosquito) using a proteomic approach to identify putative short peptides that are enriched in the midgut after blood feeding. Mosquito midguts were analyzed by two-dimensional electrophoresis to determine the changes in protein profiles. We identified 21 spot proteins that are differentially expressed in the blood of mosquitoes during the immune challenge. Molecular weight of the spots varied from 13 to 36 kDa, with a broad isoelectric point range of 3.92-8.90. We identified the differentially expressed proteins using mass spectrometry and constructed a proteomic data base of the A. albimanus midgut with diverse functions, some of them proteins with digestive and immunologic functions. Identification of these proteins may have important implications for understanding the blood meal digestion process, as well as developing novel vector control strategies and understanding parasite vector interactions. SN - 1879-0240 UR - https://www.unboundmedicine.com/medline/citation/20692341/Differential_expression_of_proteins_in_the_midgut_of_Anopheles_albimanus_infected_with_Plasmodium_berghei_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0965-1748(10)00166-9 DB - PRIME DP - Unbound Medicine ER -