Tags

Type your tag names separated by a space and hit enter

Association mapping of spot blotch resistance in wild barley.
Mol Breed. 2010 Aug; 26(2):243-256.MB

Abstract

Spot blotch, caused by Cochliobolus sativus, is an important foliar disease of barley. The disease has been controlled for over 40 years through the deployment of cultivars with durable resistance derived from the line NDB112. Pathotypes of C. sativus with virulence for the NDB112 resistance have been detected in Canada; thus, many commercial cultivars are vulnerable to spot blotch epidemics. To increase the diversity of spot blotch resistance in cultivated barley, we evaluated 318 diverse wild barley accessions comprising the Wild Barley Diversity Collection (WBDC) for reaction to C. sativus at the seedling stage and utilized an association mapping (AM) approach to identify and map resistance loci. A high frequency of resistance was found in the WBDC as 95% (302/318) of the accessions exhibited low infection responses. The WBDC was genotyped with 558 Diversity Array Technology (DArT((R))) and 2,878 single nucleotide polymorphism (SNP) markers and subjected to structure analysis before running the AM procedure. Thirteen QTL for spot blotch resistance were identified with DArT and SNP markers. These QTL were found on chromosomes 1H, 2H, 3H, 5H, and 7H and explained from 2.3 to 3.9% of the phenotypic variance. Nearly half of the identified QTL mapped to chromosome bins where spot blotch resistance loci were previously reported, offering some validation for the AM approach. The other QTL mapped to unique genomic regions and may represent new spot blotch resistance loci. This study demonstrates that AM is an effective technique for identifying and mapping QTL for disease resistance in a wild crop progenitor. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-010-9402-8) contains supplementary material, which is available to authorized users.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20694035

Citation

Roy, Joy K., et al. "Association Mapping of Spot Blotch Resistance in Wild Barley." Molecular Breeding : New Strategies in Plant Improvement, vol. 26, no. 2, 2010, pp. 243-256.
Roy JK, Smith KP, Muehlbauer GJ, et al. Association mapping of spot blotch resistance in wild barley. Mol Breed. 2010;26(2):243-256.
Roy, J. K., Smith, K. P., Muehlbauer, G. J., Chao, S., Close, T. J., & Steffenson, B. J. (2010). Association mapping of spot blotch resistance in wild barley. Molecular Breeding : New Strategies in Plant Improvement, 26(2), 243-256.
Roy JK, et al. Association Mapping of Spot Blotch Resistance in Wild Barley. Mol Breed. 2010;26(2):243-256. PubMed PMID: 20694035.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association mapping of spot blotch resistance in wild barley. AU - Roy,Joy K, AU - Smith,Kevin P, AU - Muehlbauer,Gary J, AU - Chao,Shiaoman, AU - Close,Timothy J, AU - Steffenson,Brian J, Y1 - 2010/03/10/ PY - 2009/08/16/received PY - 2010/01/25/accepted PY - 2010/8/10/entrez PY - 2010/8/10/pubmed PY - 2010/8/10/medline SP - 243 EP - 256 JF - Molecular breeding : new strategies in plant improvement JO - Mol Breed VL - 26 IS - 2 N2 - Spot blotch, caused by Cochliobolus sativus, is an important foliar disease of barley. The disease has been controlled for over 40 years through the deployment of cultivars with durable resistance derived from the line NDB112. Pathotypes of C. sativus with virulence for the NDB112 resistance have been detected in Canada; thus, many commercial cultivars are vulnerable to spot blotch epidemics. To increase the diversity of spot blotch resistance in cultivated barley, we evaluated 318 diverse wild barley accessions comprising the Wild Barley Diversity Collection (WBDC) for reaction to C. sativus at the seedling stage and utilized an association mapping (AM) approach to identify and map resistance loci. A high frequency of resistance was found in the WBDC as 95% (302/318) of the accessions exhibited low infection responses. The WBDC was genotyped with 558 Diversity Array Technology (DArT((R))) and 2,878 single nucleotide polymorphism (SNP) markers and subjected to structure analysis before running the AM procedure. Thirteen QTL for spot blotch resistance were identified with DArT and SNP markers. These QTL were found on chromosomes 1H, 2H, 3H, 5H, and 7H and explained from 2.3 to 3.9% of the phenotypic variance. Nearly half of the identified QTL mapped to chromosome bins where spot blotch resistance loci were previously reported, offering some validation for the AM approach. The other QTL mapped to unique genomic regions and may represent new spot blotch resistance loci. This study demonstrates that AM is an effective technique for identifying and mapping QTL for disease resistance in a wild crop progenitor. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-010-9402-8) contains supplementary material, which is available to authorized users. SN - 1380-3743 UR - https://www.unboundmedicine.com/medline/citation/20694035/Association_mapping_of_spot_blotch_resistance_in_wild_barley_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20694035/ DB - PRIME DP - Unbound Medicine ER -
Try the Free App:
Prime PubMed app for iOS iPhone iPad
Prime PubMed app for Android
Prime PubMed is provided
free to individuals by:
Unbound Medicine.