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Six family genes control the proliferation and differentiation of muscle satellite cells.
Exp Cell Res. 2010 Oct 15; 316(17):2932-44.EC

Abstract

Muscle satellite cells are essential for muscle growth and regeneration and their morphology, behavior and gene expression have been extensively studied. However, the mechanisms involved in their proliferation and differentiation remain elusive. Six1 and Six4 proteins were expressed in the nuclei of myofibers of adult mice and the numbers of myoblasts positive for Six1 and Six4 increased during regeneration of skeletal muscles. Six1 and Six4 were expressed in quiescent, activated and differentiated muscle satellite cells isolated from adult skeletal muscle. Overexpression of Six4 and Six5 repressed the proliferation and differentiation of satellite cells. Conversely, knockdown of Six5 resulted in augmented proliferation, and that of Six4 inhibited differentiation. Muscle satellite cells isolated from Six4(+/-)Six5(-/-) mice proliferated to higher cell density though their differentiation was not altered. Meanwhile, overproduction of Six1 repressed proliferation and promoted differentiation of satellite cells. In addition, Six4 and Six5 repressed, while Six1 activated myogenin expression, suggesting that the differential regulation of myogenin expression is responsible for the differential effects of Six genes. The results indicated the involvement of Six genes in the behavior of satellite cells and identified Six genes as potential target for manipulation of proliferation and differentiation of muscle satellite cells for therapeutic applications.

Authors+Show Affiliations

Division of Biology, Center for Molecular Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20696153

Citation

Yajima, Hiroshi, et al. "Six Family Genes Control the Proliferation and Differentiation of Muscle Satellite Cells." Experimental Cell Research, vol. 316, no. 17, 2010, pp. 2932-44.
Yajima H, Motohashi N, Ono Y, et al. Six family genes control the proliferation and differentiation of muscle satellite cells. Exp Cell Res. 2010;316(17):2932-44.
Yajima, H., Motohashi, N., Ono, Y., Sato, S., Ikeda, K., Masuda, S., Yada, E., Kanesaki, H., Miyagoe-Suzuki, Y., Takeda, S., & Kawakami, K. (2010). Six family genes control the proliferation and differentiation of muscle satellite cells. Experimental Cell Research, 316(17), 2932-44. https://doi.org/10.1016/j.yexcr.2010.08.001
Yajima H, et al. Six Family Genes Control the Proliferation and Differentiation of Muscle Satellite Cells. Exp Cell Res. 2010 Oct 15;316(17):2932-44. PubMed PMID: 20696153.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Six family genes control the proliferation and differentiation of muscle satellite cells. AU - Yajima,Hiroshi, AU - Motohashi,Norio, AU - Ono,Yusuke, AU - Sato,Shigeru, AU - Ikeda,Keiko, AU - Masuda,Satoru, AU - Yada,Erica, AU - Kanesaki,Hironori, AU - Miyagoe-Suzuki,Yuko, AU - Takeda,Shin'ichi, AU - Kawakami,Kiyoshi, Y1 - 2010/08/06/ PY - 2010/04/01/received PY - 2010/07/19/revised PY - 2010/08/03/accepted PY - 2010/8/11/entrez PY - 2010/8/11/pubmed PY - 2010/11/11/medline SP - 2932 EP - 44 JF - Experimental cell research JO - Exp. Cell Res. VL - 316 IS - 17 N2 - Muscle satellite cells are essential for muscle growth and regeneration and their morphology, behavior and gene expression have been extensively studied. However, the mechanisms involved in their proliferation and differentiation remain elusive. Six1 and Six4 proteins were expressed in the nuclei of myofibers of adult mice and the numbers of myoblasts positive for Six1 and Six4 increased during regeneration of skeletal muscles. Six1 and Six4 were expressed in quiescent, activated and differentiated muscle satellite cells isolated from adult skeletal muscle. Overexpression of Six4 and Six5 repressed the proliferation and differentiation of satellite cells. Conversely, knockdown of Six5 resulted in augmented proliferation, and that of Six4 inhibited differentiation. Muscle satellite cells isolated from Six4(+/-)Six5(-/-) mice proliferated to higher cell density though their differentiation was not altered. Meanwhile, overproduction of Six1 repressed proliferation and promoted differentiation of satellite cells. In addition, Six4 and Six5 repressed, while Six1 activated myogenin expression, suggesting that the differential regulation of myogenin expression is responsible for the differential effects of Six genes. The results indicated the involvement of Six genes in the behavior of satellite cells and identified Six genes as potential target for manipulation of proliferation and differentiation of muscle satellite cells for therapeutic applications. SN - 1090-2422 UR - https://www.unboundmedicine.com/medline/citation/20696153/Six_family_genes_control_the_proliferation_and_differentiation_of_muscle_satellite_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-4827(10)00395-2 DB - PRIME DP - Unbound Medicine ER -