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β-Galactofuranose-containing structures present in the cell wall of the saprophytic fungus Cladosporium (Hormoconis) resinae.
Res Microbiol. 2010 Oct; 161(8):720-8.RM

Abstract

A peptidogalactomannan was isolated from mycelia of Cladosporium (Hormoconis) resinae and characterized using methylation-fragmentation analysis, partial acid hydrolysis and ¹H and ¹³C-NMR spectroscopy. The galactomannan component was a branched structure and consisted of a main chain containing (1→6)-linked α-d-Manp residues substituted at O-2 by side chains containing (1→2)-linked α-D-Manp residues. β-D-Galf residues were present as side chains of 3-4 units that are (1→5)-interlinked. This structure is very similar to a pGM isolated from Aspergillus fumigatus and differs from that of Cladosporium werneckii (currently named Hortaea werneckii), with this pGM and other fungal galactomannans having single terminal (1→6)-linked β-Galf residues. The importance of the carbohydrate moiety of Cladosporium resinae pGM in immunoassays was also demonstrated. On FACS examination, a decrease (60%) in rabbit serum anti- C. resinae binding to C. resinae conidia occurred when this serum had been previously incubated with pGMs from C. resinae and A. fumigatus or with mannoprotein from Candida parapsilosis, suggesting the presence of cross-reactive determinants in these fungi.

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Authors+Show Affiliations

Calixto R
Instituto de Microbiologia, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro (UFRJ),Bloco I, Ilha do Fundão, 21941-970 Rio de Janeiro, RJ, Brazil.
Mattos B
No affiliation info available
Bittencourt V
No affiliation info available
Lopes L
No affiliation info available
Souza L
No affiliation info available
Sassaki G
No affiliation info available
Cipriani T
No affiliation info available
Silva M
No affiliation info available
Barreto-Bergter E
No affiliation info available

MeSH

AnimalsAntigens, FungalCell WallCladosporiumCross ReactionsElectrophoresis, Polyacrylamide GelEnzyme-Linked Immunosorbent AssayFlow CytometryFungal ProteinsFungiGalactoseGlycopeptidesMagnetic Resonance SpectroscopyMannansMethylationMolecular StructureMyceliumRabbits

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20696239

Citation

Calixto, Renata, et al. "Β-Galactofuranose-containing Structures Present in the Cell Wall of the Saprophytic Fungus Cladosporium (Hormoconis) Resinae." Research in Microbiology, vol. 161, no. 8, 2010, pp. 720-8.
Calixto R, Mattos B, Bittencourt V, et al. Β-Galactofuranose-containing structures present in the cell wall of the saprophytic fungus Cladosporium (Hormoconis) resinae. Res Microbiol. 2010;161(8):720-8.
Calixto, R., Mattos, B., Bittencourt, V., Lopes, L., Souza, L., Sassaki, G., Cipriani, T., Silva, M., & Barreto-Bergter, E. (2010). Β-Galactofuranose-containing structures present in the cell wall of the saprophytic fungus Cladosporium (Hormoconis) resinae. Research in Microbiology, 161(8), 720-8. https://doi.org/10.1016/j.resmic.2010.07.005
Calixto R, et al. Β-Galactofuranose-containing Structures Present in the Cell Wall of the Saprophytic Fungus Cladosporium (Hormoconis) Resinae. Res Microbiol. 2010;161(8):720-8. PubMed PMID: 20696239.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - β-Galactofuranose-containing structures present in the cell wall of the saprophytic fungus Cladosporium (Hormoconis) resinae. AU - Calixto,Renata, AU - Mattos,Bianca, AU - Bittencourt,Vera, AU - Lopes,Lívia, AU - Souza,Lauro, AU - Sassaki,Guilherme, AU - Cipriani,Thales, AU - Silva,Maria, AU - Barreto-Bergter,Eliana, Y1 - 2010/08/07/ PY - 2010/03/12/received PY - 2010/07/15/revised PY - 2010/07/18/accepted PY - 2010/8/11/entrez PY - 2010/8/11/pubmed PY - 2010/12/16/medline SP - 720 EP - 8 JF - Research in microbiology JO - Res Microbiol VL - 161 IS - 8 N2 - A peptidogalactomannan was isolated from mycelia of Cladosporium (Hormoconis) resinae and characterized using methylation-fragmentation analysis, partial acid hydrolysis and ¹H and ¹³C-NMR spectroscopy. The galactomannan component was a branched structure and consisted of a main chain containing (1→6)-linked α-d-Manp residues substituted at O-2 by side chains containing (1→2)-linked α-D-Manp residues. β-D-Galf residues were present as side chains of 3-4 units that are (1→5)-interlinked. This structure is very similar to a pGM isolated from Aspergillus fumigatus and differs from that of Cladosporium werneckii (currently named Hortaea werneckii), with this pGM and other fungal galactomannans having single terminal (1→6)-linked β-Galf residues. The importance of the carbohydrate moiety of Cladosporium resinae pGM in immunoassays was also demonstrated. On FACS examination, a decrease (60%) in rabbit serum anti- C. resinae binding to C. resinae conidia occurred when this serum had been previously incubated with pGMs from C. resinae and A. fumigatus or with mannoprotein from Candida parapsilosis, suggesting the presence of cross-reactive determinants in these fungi. SN - 1769-7123 UR - https://www.unboundmedicine.com/medline/citation/20696239/β_Galactofuranose_containing_structures_present_in_the_cell_wall_of_the_saprophytic_fungus_Cladosporium__Hormoconis__resinae_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0923-2508(10)00169-5 DB - PRIME DP - Unbound Medicine ER -