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Effect of cadmium exposure on expression of antioxidant gene transcripts in the river pufferfish, Takifugu obscurus (Tetraodontiformes).
Comp Biochem Physiol C Toxicol Pharmacol. 2010 Nov; 152(4):473-9.CB

Abstract

Cadmium (Cd) is a non-essential toxic heavy metal with the potential to induce oxidative stress. Cd toxicity and its capacity for accumulation in aquatic habitats have earned its recognition as a pollutant of immediate and widespread concern. To obtain a better understanding of oxidative stress-associated gene expression in different tissues, six antioxidant genes such as catalase (CAT), glutathione reductase (GR), glutathione peroxidase 1a (GPx1a), glutathione peroxidase 1b (GPx1b), Cu/Zn superoxide dismutase (Cu/Zn-SOD), and Mn superoxide dismutase (Mn-SOD) were cloned and fully sequenced in the river pufferfish, Takifugu obscurus. On tissue specific mRNA expression, the liver showed the highest expression when compared to other tissues, even though each antioxidant gene showed different modes of expression patterns in the examined tissues. Of the various antioxidant genes, GR was the most highly expressed in the liver, followed by CAT, GPx1, and Cu/Zn-SOD. For the time-course experiment, all the antioxidant genes were significantly induced over time except for Cu/Zn-SOD in the liver, and there was a 5-fold induction in hepatic GR, CAT, and Mn-SOD mRNA compared to the control. These findings indicate that the liver of T. obscurus has a robust antioxidant system. In addition, these results suggest that Cd exposure modulates the expression of antioxidant genes, and would indicate that the antioxidant genes would be a relevant biomarker of trace metal pollution such as Cd exposure in T. obscurus.

Authors+Show Affiliations

National Research Lab of Marine Molecular and Environmental Bioscience, Department of Chemistry, College of Natural Sciences, Hanyang University, Seoul 133-791, South Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20696273

Citation

Kim, Jin-Hyoung, et al. "Effect of Cadmium Exposure On Expression of Antioxidant Gene Transcripts in the River Pufferfish, Takifugu Obscurus (Tetraodontiformes)." Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, vol. 152, no. 4, 2010, pp. 473-9.
Kim JH, Rhee JS, Lee JS, et al. Effect of cadmium exposure on expression of antioxidant gene transcripts in the river pufferfish, Takifugu obscurus (Tetraodontiformes). Comp Biochem Physiol C Toxicol Pharmacol. 2010;152(4):473-9.
Kim, J. H., Rhee, J. S., Lee, J. S., Dahms, H. U., Lee, J., Han, K. N., & Lee, J. S. (2010). Effect of cadmium exposure on expression of antioxidant gene transcripts in the river pufferfish, Takifugu obscurus (Tetraodontiformes). Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 152(4), 473-9. https://doi.org/10.1016/j.cbpc.2010.08.002
Kim JH, et al. Effect of Cadmium Exposure On Expression of Antioxidant Gene Transcripts in the River Pufferfish, Takifugu Obscurus (Tetraodontiformes). Comp Biochem Physiol C Toxicol Pharmacol. 2010;152(4):473-9. PubMed PMID: 20696273.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of cadmium exposure on expression of antioxidant gene transcripts in the river pufferfish, Takifugu obscurus (Tetraodontiformes). AU - Kim,Jin-Hyoung, AU - Rhee,Jae-Sung, AU - Lee,Jin-Seon, AU - Dahms,Hans-Uwe, AU - Lee,Jehee, AU - Han,Kyung-Nam, AU - Lee,Jae-Seong, Y1 - 2010/08/07/ PY - 2009/12/30/received PY - 2010/08/02/revised PY - 2010/08/02/accepted PY - 2010/8/11/entrez PY - 2010/8/11/pubmed PY - 2010/12/17/medline SP - 473 EP - 9 JF - Comparative biochemistry and physiology. Toxicology & pharmacology : CBP JO - Comp Biochem Physiol C Toxicol Pharmacol VL - 152 IS - 4 N2 - Cadmium (Cd) is a non-essential toxic heavy metal with the potential to induce oxidative stress. Cd toxicity and its capacity for accumulation in aquatic habitats have earned its recognition as a pollutant of immediate and widespread concern. To obtain a better understanding of oxidative stress-associated gene expression in different tissues, six antioxidant genes such as catalase (CAT), glutathione reductase (GR), glutathione peroxidase 1a (GPx1a), glutathione peroxidase 1b (GPx1b), Cu/Zn superoxide dismutase (Cu/Zn-SOD), and Mn superoxide dismutase (Mn-SOD) were cloned and fully sequenced in the river pufferfish, Takifugu obscurus. On tissue specific mRNA expression, the liver showed the highest expression when compared to other tissues, even though each antioxidant gene showed different modes of expression patterns in the examined tissues. Of the various antioxidant genes, GR was the most highly expressed in the liver, followed by CAT, GPx1, and Cu/Zn-SOD. For the time-course experiment, all the antioxidant genes were significantly induced over time except for Cu/Zn-SOD in the liver, and there was a 5-fold induction in hepatic GR, CAT, and Mn-SOD mRNA compared to the control. These findings indicate that the liver of T. obscurus has a robust antioxidant system. In addition, these results suggest that Cd exposure modulates the expression of antioxidant genes, and would indicate that the antioxidant genes would be a relevant biomarker of trace metal pollution such as Cd exposure in T. obscurus. SN - 1532-0456 UR - https://www.unboundmedicine.com/medline/citation/20696273/Effect_of_cadmium_exposure_on_expression_of_antioxidant_gene_transcripts_in_the_river_pufferfish_Takifugu_obscurus__Tetraodontiformes__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1532-0456(10)00141-9 DB - PRIME DP - Unbound Medicine ER -