Tags

Type your tag names separated by a space and hit enter

Hospital outbreak caused by Klebsiella pneumoniae producing KPC-2 beta-lactamase resistant to colistin.
J Hosp Infect. 2010 Sep; 76(1):70-3.JH

Abstract

We describe a hospital outbreak caused by colistin-resistant Klebsiella pneumoniae producing KPC-2 beta-lactamase in two distinct medical centres. Seven clinical isolates of K. pneumoniae exhibiting resistance to carbapenems were collected from patients with hospital-acquired infection. All isolates were phenotypically positive for carbapenemase activity but negative for metallo-beta-lactamase production. PCR analysis using specific primers for bla(KPC), bla(SHV), bla(TEM) and bla(CTX-M) demonstrated that all clinical strains of K. pneumoniae from hospital A and one isolate from hospital B were genetically related and carried bla(KPC-2) in addition to bla(SHV-12). In contrast, the remaining isolate carried bla(S)(HV-5) with bla(K)(PC-2) and yielded a different profile. These results indicate the clonal spread of KPC producers between hospitals as well as the acquisition of KPC genes by different K. pneumoniae strains. All isolates were resistant to carbapenems, beta-lactams, ciprofloxacin, aminoglycosides and colistin, but intermediately susceptible to tigecycline and susceptible to gentamicin. The infection was fatal in five cases. The emergence of colistin-resistant K. pneumoniae possessing bla(KPC)(-2) underscores the implementation of strict control measures to prevent their dissemination of these organisms in hospitals.

Authors+Show Affiliations

Department of Clinical Microbiology, G. Gennimatas General Hospital, Thessaloniki, Greece.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20705205

Citation

Kontopoulou, K, et al. "Hospital Outbreak Caused By Klebsiella Pneumoniae Producing KPC-2 Beta-lactamase Resistant to Colistin." The Journal of Hospital Infection, vol. 76, no. 1, 2010, pp. 70-3.
Kontopoulou K, Protonotariou E, Vasilakos K, et al. Hospital outbreak caused by Klebsiella pneumoniae producing KPC-2 beta-lactamase resistant to colistin. J Hosp Infect. 2010;76(1):70-3.
Kontopoulou, K., Protonotariou, E., Vasilakos, K., Kriti, M., Koteli, A., Antoniadou, E., & Sofianou, D. (2010). Hospital outbreak caused by Klebsiella pneumoniae producing KPC-2 beta-lactamase resistant to colistin. The Journal of Hospital Infection, 76(1), 70-3. https://doi.org/10.1016/j.jhin.2010.03.021
Kontopoulou K, et al. Hospital Outbreak Caused By Klebsiella Pneumoniae Producing KPC-2 Beta-lactamase Resistant to Colistin. J Hosp Infect. 2010;76(1):70-3. PubMed PMID: 20705205.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hospital outbreak caused by Klebsiella pneumoniae producing KPC-2 beta-lactamase resistant to colistin. AU - Kontopoulou,K, AU - Protonotariou,E, AU - Vasilakos,K, AU - Kriti,M, AU - Koteli,A, AU - Antoniadou,E, AU - Sofianou,D, Y1 - 2010/06/17/ PY - 2009/07/15/received PY - 2010/03/26/accepted PY - 2010/8/14/entrez PY - 2010/8/14/pubmed PY - 2010/11/17/medline SP - 70 EP - 3 JF - The Journal of hospital infection JO - J Hosp Infect VL - 76 IS - 1 N2 - We describe a hospital outbreak caused by colistin-resistant Klebsiella pneumoniae producing KPC-2 beta-lactamase in two distinct medical centres. Seven clinical isolates of K. pneumoniae exhibiting resistance to carbapenems were collected from patients with hospital-acquired infection. All isolates were phenotypically positive for carbapenemase activity but negative for metallo-beta-lactamase production. PCR analysis using specific primers for bla(KPC), bla(SHV), bla(TEM) and bla(CTX-M) demonstrated that all clinical strains of K. pneumoniae from hospital A and one isolate from hospital B were genetically related and carried bla(KPC-2) in addition to bla(SHV-12). In contrast, the remaining isolate carried bla(S)(HV-5) with bla(K)(PC-2) and yielded a different profile. These results indicate the clonal spread of KPC producers between hospitals as well as the acquisition of KPC genes by different K. pneumoniae strains. All isolates were resistant to carbapenems, beta-lactams, ciprofloxacin, aminoglycosides and colistin, but intermediately susceptible to tigecycline and susceptible to gentamicin. The infection was fatal in five cases. The emergence of colistin-resistant K. pneumoniae possessing bla(KPC)(-2) underscores the implementation of strict control measures to prevent their dissemination of these organisms in hospitals. SN - 1532-2939 UR - https://www.unboundmedicine.com/medline/citation/20705205/Hospital_outbreak_caused_by_Klebsiella_pneumoniae_producing_KPC_2_beta_lactamase_resistant_to_colistin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0195-6701(10)00183-0 DB - PRIME DP - Unbound Medicine ER -