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Assessment of chronological lifespan dependent molecular damages in yeast lacking mitochondrial antioxidant genes.
Biochem Biophys Res Commun. 2010 Sep 10; 400(1):106-10.BB

Abstract

The free radical theory of aging states that oxidative damage to biomolecules causes aging and that antioxidants neutralize free radicals and thus decelerate aging. Mitochondria produce most of the reactive oxygen species, but at the same time have many antioxidant enzymes providing protection from these oxidants. Expecting that cells without mitochondrial antioxidant genes would accumulate higher levels of oxidative damage and, therefore, will have a shorter lifespan, we analyzed oxidative damages to biomolecules in young and chronologically aged mutants lacking the mitochondrial antioxidant genes: GRX2, CCP1, SOD1, GLO4, TRR2, TRX3, CCS1, SOD2, GRX5, and PRX1. Among these mutants, ccp1Δ, trx3Δ, grx5Δ, prx1Δ, mutants were sensitive to diamide, and ccs1Δ and sod2Δ were sensitive to both diamide and menadione. Most of the mutants were less viable in stationary phase. Chronologically aged cells produced higher amount of superoxide radical and accumulated higher levels of oxidative damages. Even though our results support the findings that old cells harbor higher amount of molecular damages, no significant difference was observed between wild type and mutant cells in terms of their damage content.

Authors+Show Affiliations

Department of Molecular Biology and Genetics, Izmir Institute of Technology, 35430 Urla, Izmir, Turkey.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20707985

Citation

Demir, Ayse Banu, and Ahmet Koc. "Assessment of Chronological Lifespan Dependent Molecular Damages in Yeast Lacking Mitochondrial Antioxidant Genes." Biochemical and Biophysical Research Communications, vol. 400, no. 1, 2010, pp. 106-10.
Demir AB, Koc A. Assessment of chronological lifespan dependent molecular damages in yeast lacking mitochondrial antioxidant genes. Biochem Biophys Res Commun. 2010;400(1):106-10.
Demir, A. B., & Koc, A. (2010). Assessment of chronological lifespan dependent molecular damages in yeast lacking mitochondrial antioxidant genes. Biochemical and Biophysical Research Communications, 400(1), 106-10. https://doi.org/10.1016/j.bbrc.2010.08.019
Demir AB, Koc A. Assessment of Chronological Lifespan Dependent Molecular Damages in Yeast Lacking Mitochondrial Antioxidant Genes. Biochem Biophys Res Commun. 2010 Sep 10;400(1):106-10. PubMed PMID: 20707985.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Assessment of chronological lifespan dependent molecular damages in yeast lacking mitochondrial antioxidant genes. AU - Demir,Ayse Banu, AU - Koc,Ahmet, Y1 - 2010/08/11/ PY - 2010/08/04/received PY - 2010/08/04/accepted PY - 2010/8/17/entrez PY - 2010/8/17/pubmed PY - 2010/10/12/medline SP - 106 EP - 10 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 400 IS - 1 N2 - The free radical theory of aging states that oxidative damage to biomolecules causes aging and that antioxidants neutralize free radicals and thus decelerate aging. Mitochondria produce most of the reactive oxygen species, but at the same time have many antioxidant enzymes providing protection from these oxidants. Expecting that cells without mitochondrial antioxidant genes would accumulate higher levels of oxidative damage and, therefore, will have a shorter lifespan, we analyzed oxidative damages to biomolecules in young and chronologically aged mutants lacking the mitochondrial antioxidant genes: GRX2, CCP1, SOD1, GLO4, TRR2, TRX3, CCS1, SOD2, GRX5, and PRX1. Among these mutants, ccp1Δ, trx3Δ, grx5Δ, prx1Δ, mutants were sensitive to diamide, and ccs1Δ and sod2Δ were sensitive to both diamide and menadione. Most of the mutants were less viable in stationary phase. Chronologically aged cells produced higher amount of superoxide radical and accumulated higher levels of oxidative damages. Even though our results support the findings that old cells harbor higher amount of molecular damages, no significant difference was observed between wild type and mutant cells in terms of their damage content. SN - 1090-2104 UR - https://www.unboundmedicine.com/medline/citation/20707985/Assessment_of_chronological_lifespan_dependent_molecular_damages_in_yeast_lacking_mitochondrial_antioxidant_genes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-291X(10)01505-6 DB - PRIME DP - Unbound Medicine ER -