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A signal-on electrochemical probe-label-free aptasensor using gold-platinum alloy and stearic acid as enhancers.
Biosens Bioelectron. 2010 Oct 15; 26(2):881-5.BB

Abstract

In the present study, a new electrochemical probe-label-free aptasensor for thrombin (TB) based on the amplification of gold-platinum alloy nanoparticles (Au-PtNPs) and stearic acid was reported. Nafion@multiwalled carbon nanotubes (MWCNTs) coated electrode was firstly modified with electrochemical probe of methylene blue (MB). Then, Au-PtNPs were electrodeposited onto the electrode surface for the immobilization of aptamer and further hybridization of stearic acid labeled thrombin aptamer (TBA). In the presence of TB, the TBA bound with TB and released from the self-assembled duplex on the electrode into solution, decreasing the steric hindrance of the aptasensor and facilitating catalytic efficiency of Au-PtNPs in the presence of MB toward H2O2 with an enhanced electrochemical signal. With the enhanced effect of Au-PtNPs/MB modified electrode and stearic acid, a detection limit as low as 3pM for TB was achieved. The aptasensor also exhibited good selectivity and reproducibility.

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Authors+Show Affiliations

Yuan Y
Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, College of Chemistry and Chemical Engineering,Southwest University, Chongqing 400715, PR China.
Yuan R
No affiliation info available
Chai Y
No affiliation info available
Zhuo Y
No affiliation info available
Bai L
No affiliation info available
Liao Y
No affiliation info available

MeSH

AlloysAptamers, NucleotideBiosensing TechniquesConductometryEquipment DesignEquipment Failure AnalysisGoldPlatinumStaining and LabelingStearic AcidsThrombin

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20708400

Citation

Yuan, Yali, et al. "A Signal-on Electrochemical Probe-label-free Aptasensor Using Gold-platinum Alloy and Stearic Acid as Enhancers." Biosensors & Bioelectronics, vol. 26, no. 2, 2010, pp. 881-5.
Yuan Y, Yuan R, Chai Y, et al. A signal-on electrochemical probe-label-free aptasensor using gold-platinum alloy and stearic acid as enhancers. Biosens Bioelectron. 2010;26(2):881-5.
Yuan, Y., Yuan, R., Chai, Y., Zhuo, Y., Bai, L., & Liao, Y. (2010). A signal-on electrochemical probe-label-free aptasensor using gold-platinum alloy and stearic acid as enhancers. Biosensors & Bioelectronics, 26(2), 881-5. https://doi.org/10.1016/j.bios.2010.07.035
Yuan Y, et al. A Signal-on Electrochemical Probe-label-free Aptasensor Using Gold-platinum Alloy and Stearic Acid as Enhancers. Biosens Bioelectron. 2010 Oct 15;26(2):881-5. PubMed PMID: 20708400.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A signal-on electrochemical probe-label-free aptasensor using gold-platinum alloy and stearic acid as enhancers. AU - Yuan,Yali, AU - Yuan,Ruo, AU - Chai,Yaqin, AU - Zhuo,Ying, AU - Bai,Lijuan, AU - Liao,Yuhong, Y1 - 2010/07/16/ PY - 2010/05/16/received PY - 2010/07/07/revised PY - 2010/07/11/accepted PY - 2010/8/17/entrez PY - 2010/8/17/pubmed PY - 2011/2/8/medline SP - 881 EP - 5 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 26 IS - 2 N2 - In the present study, a new electrochemical probe-label-free aptasensor for thrombin (TB) based on the amplification of gold-platinum alloy nanoparticles (Au-PtNPs) and stearic acid was reported. Nafion@multiwalled carbon nanotubes (MWCNTs) coated electrode was firstly modified with electrochemical probe of methylene blue (MB). Then, Au-PtNPs were electrodeposited onto the electrode surface for the immobilization of aptamer and further hybridization of stearic acid labeled thrombin aptamer (TBA). In the presence of TB, the TBA bound with TB and released from the self-assembled duplex on the electrode into solution, decreasing the steric hindrance of the aptasensor and facilitating catalytic efficiency of Au-PtNPs in the presence of MB toward H2O2 with an enhanced electrochemical signal. With the enhanced effect of Au-PtNPs/MB modified electrode and stearic acid, a detection limit as low as 3pM for TB was achieved. The aptasensor also exhibited good selectivity and reproducibility. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/20708400/A_signal_on_electrochemical_probe_label_free_aptasensor_using_gold_platinum_alloy_and_stearic_acid_as_enhancers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(10)00410-0 DB - PRIME DP - Unbound Medicine ER -