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4-1BB-mediated expansion affords superior detection of in vivo primed effector memory CD8+ T cells from melanoma sentinel lymph nodes.
Clin Immunol. 2010 Nov; 137(2):221-33.CI

Abstract

We have been studying the re-activation of tumor-associated antigen (TAA)-specific CD8(+) T cells in sentinel lymph nodes (SLN) of melanoma patients upon intradermal administration of the CpG-B oligodeoxynucleotide PF-3512676. To facilitate functional testing of T cells from small SLN samples, high-efficiency polyclonal T cell expansion is required. In this study, SLN cells were expanded via classic methodologies with plate- or bead-bound anti-CD3/CD28 antibodies and with the K562/CD32/4-1BBL artificial APC system (K32/4-1BBL aAPC) and analyzed for responsiveness to common recall or TAA-derived peptides. K32/4-1BBL-expanded T cell populations contained significantly more effector/memory CD8(+) T cells. Moreover, recall and melanoma antigen-specific CD8(+) T cells were more frequently detected in K32/4-1BBL-expanded samples as compared with anti-CD3/CD28-expanded samples. We conclude that K32/4-1BBL aAPC are superior to anti-CD3/CD28 antibodies for the expansion of in vivo-primed specific CD8(+) T cells and that their use facilitates the sensitive monitoring of functional anti-tumor T cell immunity in SLN.

Authors+Show Affiliations

Department of Surgical Oncology, VU University Medical Center, Cancer Center Amsterdam, Amsterdam, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20708974

Citation

Sluijter, B J R., et al. "4-1BB-mediated Expansion Affords Superior Detection of in Vivo Primed Effector Memory CD8+ T Cells From Melanoma Sentinel Lymph Nodes." Clinical Immunology (Orlando, Fla.), vol. 137, no. 2, 2010, pp. 221-33.
Sluijter BJ, van den Hout MF, Stam AG, et al. 4-1BB-mediated expansion affords superior detection of in vivo primed effector memory CD8+ T cells from melanoma sentinel lymph nodes. Clin Immunol. 2010;137(2):221-33.
Sluijter, B. J., van den Hout, M. F., Stam, A. G., Lougheed, S. M., Suhoski, M. M., van den Eertwegh, A. J., van den Tol, M. P., van Leeuwen, P. A., Meijer, S., Scheper, R. J., June, C. H., de Gruijl, T. D., & Santegoets, S. J. (2010). 4-1BB-mediated expansion affords superior detection of in vivo primed effector memory CD8+ T cells from melanoma sentinel lymph nodes. Clinical Immunology (Orlando, Fla.), 137(2), 221-33. https://doi.org/10.1016/j.clim.2010.07.009
Sluijter BJ, et al. 4-1BB-mediated Expansion Affords Superior Detection of in Vivo Primed Effector Memory CD8+ T Cells From Melanoma Sentinel Lymph Nodes. Clin Immunol. 2010;137(2):221-33. PubMed PMID: 20708974.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 4-1BB-mediated expansion affords superior detection of in vivo primed effector memory CD8+ T cells from melanoma sentinel lymph nodes. AU - Sluijter,B J R, AU - van den Hout,M F C M, AU - Stam,A G M, AU - Lougheed,S M, AU - Suhoski,M M, AU - van den Eertwegh,A J M, AU - van den Tol,M P, AU - van Leeuwen,P A M, AU - Meijer,S, AU - Scheper,R J, AU - June,C H, AU - de Gruijl,T D, AU - Santegoets,S J A M, Y1 - 2010/08/13/ PY - 2010/06/09/received PY - 2010/07/23/revised PY - 2010/07/24/accepted PY - 2010/8/17/entrez PY - 2010/8/17/pubmed PY - 2011/2/18/medline SP - 221 EP - 33 JF - Clinical immunology (Orlando, Fla.) JO - Clin. Immunol. VL - 137 IS - 2 N2 - We have been studying the re-activation of tumor-associated antigen (TAA)-specific CD8(+) T cells in sentinel lymph nodes (SLN) of melanoma patients upon intradermal administration of the CpG-B oligodeoxynucleotide PF-3512676. To facilitate functional testing of T cells from small SLN samples, high-efficiency polyclonal T cell expansion is required. In this study, SLN cells were expanded via classic methodologies with plate- or bead-bound anti-CD3/CD28 antibodies and with the K562/CD32/4-1BBL artificial APC system (K32/4-1BBL aAPC) and analyzed for responsiveness to common recall or TAA-derived peptides. K32/4-1BBL-expanded T cell populations contained significantly more effector/memory CD8(+) T cells. Moreover, recall and melanoma antigen-specific CD8(+) T cells were more frequently detected in K32/4-1BBL-expanded samples as compared with anti-CD3/CD28-expanded samples. We conclude that K32/4-1BBL aAPC are superior to anti-CD3/CD28 antibodies for the expansion of in vivo-primed specific CD8(+) T cells and that their use facilitates the sensitive monitoring of functional anti-tumor T cell immunity in SLN. SN - 1521-7035 UR - https://www.unboundmedicine.com/medline/citation/20708974/4_1BB_mediated_expansion_affords_superior_detection_of_in_vivo_primed_effector_memory_CD8+_T_cells_from_melanoma_sentinel_lymph_nodes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1521-6616(10)00675-3 DB - PRIME DP - Unbound Medicine ER -