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Intermediate conductance Ca2+ activated K+ channels are expressed and functional in breast adenocarcinomas: correlation with tumour grade and metastasis status.
Histol Histopathol. 2010 10; 25(10):1247-55.HH

Abstract

K+ channels are key molecules in the progression of several cancer types and are considered to be potential targets for cancer therapy. In this study, we investigated the intermediate- conductance Ca2+-activated K+ channels (hKCa3.1) expression in both breast carcinoma (BC) specimens and human breast cancer epithelial primary cell cultures (hBCE) using immuno-histochemistry (60 samples), quantitative Real-Time RT-PCR (30 samples) and Western blot assay (30 samples). We also looked at whether or not the expression of these channels is correlated with breast carcinomas grade tumours and metastasis status. Furthermore, we characterized the hKCa3.1 channel activity in hBCE cells by using the Whole Cell Patch Clamp Technique. We found that hKCa3.1 transcripts and proteins were expressed in both BC samples and hBCE cells. Clinicopathologic evaluation indicated a significant correlation between hKCa3.1-expression and tumour grade. hKCa3.1 mRNA and protein were more highly expressed in grade III tumours than in both grades I and II. However, the hKCa3.1 expression-increase according to grade was only observed in tumours with negative metastasis status. Moreover, the hKCa3.1 channels expressed in hBCE cells are functional. This was attested by patch-clamp recordings showing typical hKCa3.1-mediated currents in these cells. In conclusion, these data suggest that hKCa3.1 might contribute to breast tumour-progression and can serve as a useful prognostic marker for breast cancer.

Authors+Show Affiliations

LPCM JE 2530, UPJV, Amiens, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20712009

Citation

Haren, Nathalie, et al. "Intermediate Conductance Ca2+ Activated K+ Channels Are Expressed and Functional in Breast Adenocarcinomas: Correlation With Tumour Grade and Metastasis Status." Histology and Histopathology, vol. 25, no. 10, 2010, pp. 1247-55.
Haren N, Khorsi H, Faouzi M, et al. Intermediate conductance Ca2+ activated K+ channels are expressed and functional in breast adenocarcinomas: correlation with tumour grade and metastasis status. Histol Histopathol. 2010;25(10):1247-55.
Haren, N., Khorsi, H., Faouzi, M., Ahidouch, A., Sevestre, H., & Ouadid-Ahidouch, H. (2010). Intermediate conductance Ca2+ activated K+ channels are expressed and functional in breast adenocarcinomas: correlation with tumour grade and metastasis status. Histology and Histopathology, 25(10), 1247-55. https://doi.org/10.14670/HH-25.1247
Haren N, et al. Intermediate Conductance Ca2+ Activated K+ Channels Are Expressed and Functional in Breast Adenocarcinomas: Correlation With Tumour Grade and Metastasis Status. Histol Histopathol. 2010;25(10):1247-55. PubMed PMID: 20712009.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intermediate conductance Ca2+ activated K+ channels are expressed and functional in breast adenocarcinomas: correlation with tumour grade and metastasis status. AU - Haren,Nathalie, AU - Khorsi,Hafida, AU - Faouzi,Malika, AU - Ahidouch,Ahmed, AU - Sevestre,Henri, AU - Ouadid-Ahidouch,Halima, PY - 2010/8/17/entrez PY - 2010/8/17/pubmed PY - 2010/12/14/medline SP - 1247 EP - 55 JF - Histology and histopathology JO - Histol Histopathol VL - 25 IS - 10 N2 - K+ channels are key molecules in the progression of several cancer types and are considered to be potential targets for cancer therapy. In this study, we investigated the intermediate- conductance Ca2+-activated K+ channels (hKCa3.1) expression in both breast carcinoma (BC) specimens and human breast cancer epithelial primary cell cultures (hBCE) using immuno-histochemistry (60 samples), quantitative Real-Time RT-PCR (30 samples) and Western blot assay (30 samples). We also looked at whether or not the expression of these channels is correlated with breast carcinomas grade tumours and metastasis status. Furthermore, we characterized the hKCa3.1 channel activity in hBCE cells by using the Whole Cell Patch Clamp Technique. We found that hKCa3.1 transcripts and proteins were expressed in both BC samples and hBCE cells. Clinicopathologic evaluation indicated a significant correlation between hKCa3.1-expression and tumour grade. hKCa3.1 mRNA and protein were more highly expressed in grade III tumours than in both grades I and II. However, the hKCa3.1 expression-increase according to grade was only observed in tumours with negative metastasis status. Moreover, the hKCa3.1 channels expressed in hBCE cells are functional. This was attested by patch-clamp recordings showing typical hKCa3.1-mediated currents in these cells. In conclusion, these data suggest that hKCa3.1 might contribute to breast tumour-progression and can serve as a useful prognostic marker for breast cancer. SN - 1699-5848 UR - https://www.unboundmedicine.com/medline/citation/20712009/Intermediate_conductance_Ca2+_activated_K+_channels_are_expressed_and_functional_in_breast_adenocarcinomas:_correlation_with_tumour_grade_and_metastasis_status_ L2 - http://www.hh.um.es/Abstracts/Vol_25/25_10/25_10_1247.htm DB - PRIME DP - Unbound Medicine ER -