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EST-derived SSR markers used as anchor loci for the construction of a consensus linkage map in ryegrass (Lolium spp.).
BMC Plant Biol. 2010 Aug 16; 10:177.BP

Abstract

BACKGROUND

Genetic markers and linkage mapping are basic prerequisites for marker-assisted selection and map-based cloning. In the case of the key grassland species Lolium spp., numerous mapping populations have been developed and characterised for various traits. Although some genetic linkage maps of these populations have been aligned with each other using publicly available DNA markers, the number of common markers among genetic maps is still low, limiting the ability to compare candidate gene and QTL locations across germplasm.

RESULTS

A set of 204 expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers has been assigned to map positions using eight different ryegrass mapping populations. Marker properties of a subset of 64 EST-SSRs were assessed in six to eight individuals of each mapping population and revealed 83% of the markers to be polymorphic in at least one population and an average number of alleles of 4.88. EST-SSR markers polymorphic in multiple populations served as anchor markers and allowed the construction of the first comprehensive consensus map for ryegrass. The integrated map was complemented with 97 SSRs from previously published linkage maps and finally contained 284 EST-derived and genomic SSR markers. The total map length was 742 centiMorgan (cM), ranging for individual chromosomes from 70 cM of linkage group (LG) 6 to 171 cM of LG 2.

CONCLUSIONS

The consensus linkage map for ryegrass based on eight mapping populations and constructed using a large set of publicly available Lolium EST-SSRs mapped for the first time together with previously mapped SSR markers will allow for consolidating existing mapping and QTL information in ryegrass. Map and markers presented here will prove to be an asset in the development for both molecular breeding of ryegrass as well as comparative genetics and genomics within grass species.

Authors+Show Affiliations

Department of Genetics and Biotechnology, Faculty of Agricultural Sciences, Research Centre Flakkebjerg, Aarhus University, Forsøgsvej 1, 4200 Slagelse, Denmark. bruno.studer@agrsci.dkNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20712870

Citation

Studer, Bruno, et al. "EST-derived SSR Markers Used as Anchor Loci for the Construction of a Consensus Linkage Map in Ryegrass (Lolium Spp.)." BMC Plant Biology, vol. 10, 2010, p. 177.
Studer B, Kölliker R, Muylle H, et al. EST-derived SSR markers used as anchor loci for the construction of a consensus linkage map in ryegrass (Lolium spp.). BMC Plant Biol. 2010;10:177.
Studer, B., Kölliker, R., Muylle, H., Asp, T., Frei, U., Roldán-Ruiz, I., Barre, P., Tomaszewski, C., Meally, H., Barth, S., Skøt, L., Armstead, I. P., Dolstra, O., & Lübberstedt, T. (2010). EST-derived SSR markers used as anchor loci for the construction of a consensus linkage map in ryegrass (Lolium spp.). BMC Plant Biology, 10, 177. https://doi.org/10.1186/1471-2229-10-177
Studer B, et al. EST-derived SSR Markers Used as Anchor Loci for the Construction of a Consensus Linkage Map in Ryegrass (Lolium Spp.). BMC Plant Biol. 2010 Aug 16;10:177. PubMed PMID: 20712870.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - EST-derived SSR markers used as anchor loci for the construction of a consensus linkage map in ryegrass (Lolium spp.). AU - Studer,Bruno, AU - Kölliker,Roland, AU - Muylle,Hilde, AU - Asp,Torben, AU - Frei,Ursula, AU - Roldán-Ruiz,Isabel, AU - Barre,Philippe, AU - Tomaszewski,Céline, AU - Meally,Helena, AU - Barth,Susanne, AU - Skøt,Leif, AU - Armstead,Ian P, AU - Dolstra,Oene, AU - Lübberstedt,Thomas, Y1 - 2010/08/16/ PY - 2010/01/22/received PY - 2010/08/16/accepted PY - 2010/8/18/entrez PY - 2010/8/18/pubmed PY - 2010/12/14/medline SP - 177 EP - 177 JF - BMC plant biology JO - BMC Plant Biol. VL - 10 N2 - BACKGROUND: Genetic markers and linkage mapping are basic prerequisites for marker-assisted selection and map-based cloning. In the case of the key grassland species Lolium spp., numerous mapping populations have been developed and characterised for various traits. Although some genetic linkage maps of these populations have been aligned with each other using publicly available DNA markers, the number of common markers among genetic maps is still low, limiting the ability to compare candidate gene and QTL locations across germplasm. RESULTS: A set of 204 expressed sequence tag (EST)-derived simple sequence repeat (SSR) markers has been assigned to map positions using eight different ryegrass mapping populations. Marker properties of a subset of 64 EST-SSRs were assessed in six to eight individuals of each mapping population and revealed 83% of the markers to be polymorphic in at least one population and an average number of alleles of 4.88. EST-SSR markers polymorphic in multiple populations served as anchor markers and allowed the construction of the first comprehensive consensus map for ryegrass. The integrated map was complemented with 97 SSRs from previously published linkage maps and finally contained 284 EST-derived and genomic SSR markers. The total map length was 742 centiMorgan (cM), ranging for individual chromosomes from 70 cM of linkage group (LG) 6 to 171 cM of LG 2. CONCLUSIONS: The consensus linkage map for ryegrass based on eight mapping populations and constructed using a large set of publicly available Lolium EST-SSRs mapped for the first time together with previously mapped SSR markers will allow for consolidating existing mapping and QTL information in ryegrass. Map and markers presented here will prove to be an asset in the development for both molecular breeding of ryegrass as well as comparative genetics and genomics within grass species. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/20712870/EST_derived_SSR_markers_used_as_anchor_loci_for_the_construction_of_a_consensus_linkage_map_in_ryegrass__Lolium_spp___ L2 - https://bmcplantbiol.biomedcentral.com/articles/10.1186/1471-2229-10-177 DB - PRIME DP - Unbound Medicine ER -