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Silencing of two alternative splicing-derived mRNA variants of chitin synthase 1 gene by RNAi is lethal to the oriental migratory locust, Locusta migratoria manilensis (Meyen).
Insect Biochem Mol Biol. 2010 Nov; 40(11):824-33.IB

Abstract

Chitin synthases are crucial enzymes responsible for chitin biosynthesis in fungi, nematodes and arthropods. We characterized two alternative splicing-derived variants of chitin synthase 1 gene (LmCHS1) from the oriental migratory locust, Locusta migratoria manilensis (Meyen). Each cDNA of the two variants (LmCHS1A and LmCHS1B) consists of 5116 nucleotides that include a 4728-nucleotide open reading frame (ORF) encoding 1576 amino acid residues, and 67- and 321-bp non-coding regions at the 5'- and 3'-ends of the cDNA, respectively. The two variants differ only in one exon consisting of 177 nucleotides that encode 59 amino acid residues. The amino acid sequences within this alternative splicing region are 75% identical between the two variants. Both variants were expressed in all the developmental stages. However, LmCHS1A was predominately expressed in the integument whereas LmCHS1B was mainly expressed in the trachea. Our RNAi-based gene silencing study resulted in a dramatic reduction in the levels of the corresponding mRNA in the locust nymphs injected with dsRNA of LmCHS1, or either of its two variants, LmCHS1A and LmCHS1B. Consequentially, 95, 88 and 51% of mortalities were observed in the locusts injected with the LmCHS1, LmCHS1A and LmCHS1B dsRNA, respectively. The phenotypes resulted from the injection of LmCHS1A dsRNA were similar to those from the injection of LmCHS1 dsRNA, whereas the locusts injected with LmCHS1B dsRNA exhibited crimpled cuticle phenotype. Our results suggest that both variants of chitin synthase 1 are essential for insect growth and development.

Authors+Show Affiliations

Research Institute of Applied Biology, Shanxi University, Taiyuan, Shanxi 030006, China. zjz@sxu.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20713155

Citation

Zhang, Jianzhen, et al. "Silencing of Two Alternative Splicing-derived mRNA Variants of Chitin Synthase 1 Gene By RNAi Is Lethal to the Oriental Migratory Locust, Locusta Migratoria Manilensis (Meyen)." Insect Biochemistry and Molecular Biology, vol. 40, no. 11, 2010, pp. 824-33.
Zhang J, Liu X, Zhang J, et al. Silencing of two alternative splicing-derived mRNA variants of chitin synthase 1 gene by RNAi is lethal to the oriental migratory locust, Locusta migratoria manilensis (Meyen). Insect Biochem Mol Biol. 2010;40(11):824-33.
Zhang, J., Liu, X., Zhang, J., Li, D., Sun, Y., Guo, Y., Ma, E., & Zhu, K. Y. (2010). Silencing of two alternative splicing-derived mRNA variants of chitin synthase 1 gene by RNAi is lethal to the oriental migratory locust, Locusta migratoria manilensis (Meyen). Insect Biochemistry and Molecular Biology, 40(11), 824-33. https://doi.org/10.1016/j.ibmb.2010.08.001
Zhang J, et al. Silencing of Two Alternative Splicing-derived mRNA Variants of Chitin Synthase 1 Gene By RNAi Is Lethal to the Oriental Migratory Locust, Locusta Migratoria Manilensis (Meyen). Insect Biochem Mol Biol. 2010;40(11):824-33. PubMed PMID: 20713155.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Silencing of two alternative splicing-derived mRNA variants of chitin synthase 1 gene by RNAi is lethal to the oriental migratory locust, Locusta migratoria manilensis (Meyen). AU - Zhang,Jianzhen, AU - Liu,Xiaojian, AU - Zhang,Jianqin, AU - Li,Daqi, AU - Sun,Yi, AU - Guo,Yaping, AU - Ma,Enbo, AU - Zhu,Kun Yan, Y1 - 2010/08/14/ PY - 2010/05/08/received PY - 2010/06/28/revised PY - 2010/08/04/accepted PY - 2010/8/18/entrez PY - 2010/8/18/pubmed PY - 2011/1/21/medline SP - 824 EP - 33 JF - Insect biochemistry and molecular biology JO - Insect Biochem Mol Biol VL - 40 IS - 11 N2 - Chitin synthases are crucial enzymes responsible for chitin biosynthesis in fungi, nematodes and arthropods. We characterized two alternative splicing-derived variants of chitin synthase 1 gene (LmCHS1) from the oriental migratory locust, Locusta migratoria manilensis (Meyen). Each cDNA of the two variants (LmCHS1A and LmCHS1B) consists of 5116 nucleotides that include a 4728-nucleotide open reading frame (ORF) encoding 1576 amino acid residues, and 67- and 321-bp non-coding regions at the 5'- and 3'-ends of the cDNA, respectively. The two variants differ only in one exon consisting of 177 nucleotides that encode 59 amino acid residues. The amino acid sequences within this alternative splicing region are 75% identical between the two variants. Both variants were expressed in all the developmental stages. However, LmCHS1A was predominately expressed in the integument whereas LmCHS1B was mainly expressed in the trachea. Our RNAi-based gene silencing study resulted in a dramatic reduction in the levels of the corresponding mRNA in the locust nymphs injected with dsRNA of LmCHS1, or either of its two variants, LmCHS1A and LmCHS1B. Consequentially, 95, 88 and 51% of mortalities were observed in the locusts injected with the LmCHS1, LmCHS1A and LmCHS1B dsRNA, respectively. The phenotypes resulted from the injection of LmCHS1A dsRNA were similar to those from the injection of LmCHS1 dsRNA, whereas the locusts injected with LmCHS1B dsRNA exhibited crimpled cuticle phenotype. Our results suggest that both variants of chitin synthase 1 are essential for insect growth and development. SN - 1879-0240 UR - https://www.unboundmedicine.com/medline/citation/20713155/Silencing_of_two_alternative_splicing_derived_mRNA_variants_of_chitin_synthase_1_gene_by_RNAi_is_lethal_to_the_oriental_migratory_locust_Locusta_migratoria_manilensis__Meyen__ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0965-1748(10)00169-4 DB - PRIME DP - Unbound Medicine ER -