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Caries experience and quantification of Streptococcus mutans and Streptococcus sobrinus in saliva of Sudanese schoolchildren.
Caries Res. 2010; 44(4):402-7.CR

Abstract

Streptococcus mutans and Streptococcus sobrinus are among the most commonly isolated bacterial species implicated as etiological agents of dental caries. Details of the composition of the oral microflora related to dental caries should aid in assessing the prevalence and risk of disease at an individual level. The aim of the present study was to determine the presence and relative amounts of S. mutans and S. sobrinus in the saliva samples obtained from schoolchildren in Khartoum State, the Sudan, and to study the association of the amounts of S. mutans and S. sobrinus with caries experience, socioeconomic status and sugar-sweetened snacks in this population. 140 samples, 30 of which were from individuals with caries experience, were examined using quantitative real-time polymerase chain reaction (qRT-PCR) with specific oligonucleotide primers. The mean ratio of fold differences of S. mutans to S. sobrinus was 0.77 (SD 5.4) and 2.29 (SD 6.0) for samples obtained from caries-free and caries-active individuals, respectively. This suggested that the proportion of S. sobrinus was higher than S.mutans in the caries-active group when compared to the caries-free group. An association was found between children with caries-active lesions and the frequent consumption of sticky desserts and higher socioeconomic status. S. sobrinus seems to be associated with caries experience in the studied population. A proposal of caries screening programs designed to test for S. sobrinus in this population may be developed.

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Authors+Show Affiliations

Nurelhuda NM
Section for Microbiology and Immunology, The Gade Institute, Faculty of Medicine and Dentistry, University of Bergen, Bergen, Norway. n.nurelhuda@hotmail.co.uk
Al-Haroni M
No affiliation info available
Trovik TA
No affiliation info available
Bakken V
No affiliation info available

MeSH

Case-Control StudiesChildDental CariesDental Caries SusceptibilityDiet, CariogenicFemaleHumansMaleMicrobial InteractionsRNA, BacterialRNA, Ribosomal, 16SReference ValuesReverse Transcriptase Polymerase Chain ReactionSalivaSocial ClassStatistics, NonparametricStreptococcus mutansStreptococcus sobrinusSudan

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20714152

Citation

Nurelhuda, Nazik M., et al. "Caries Experience and Quantification of Streptococcus Mutans and Streptococcus Sobrinus in Saliva of Sudanese Schoolchildren." Caries Research, vol. 44, no. 4, 2010, pp. 402-7.
Nurelhuda NM, Al-Haroni M, Trovik TA, et al. Caries experience and quantification of Streptococcus mutans and Streptococcus sobrinus in saliva of Sudanese schoolchildren. Caries Res. 2010;44(4):402-7.
Nurelhuda, N. M., Al-Haroni, M., Trovik, T. A., & Bakken, V. (2010). Caries experience and quantification of Streptococcus mutans and Streptococcus sobrinus in saliva of Sudanese schoolchildren. Caries Research, 44(4), 402-7. https://doi.org/10.1159/000316664
Nurelhuda NM, et al. Caries Experience and Quantification of Streptococcus Mutans and Streptococcus Sobrinus in Saliva of Sudanese Schoolchildren. Caries Res. 2010;44(4):402-7. PubMed PMID: 20714152.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Caries experience and quantification of Streptococcus mutans and Streptococcus sobrinus in saliva of Sudanese schoolchildren. AU - Nurelhuda,Nazik M, AU - Al-Haroni,M, AU - Trovik,T A, AU - Bakken,V, Y1 - 2010/08/12/ PY - 2009/10/15/received PY - 2010/06/09/accepted PY - 2010/8/18/entrez PY - 2010/8/18/pubmed PY - 2011/3/29/medline SP - 402 EP - 7 JF - Caries research JO - Caries Res VL - 44 IS - 4 N2 - Streptococcus mutans and Streptococcus sobrinus are among the most commonly isolated bacterial species implicated as etiological agents of dental caries. Details of the composition of the oral microflora related to dental caries should aid in assessing the prevalence and risk of disease at an individual level. The aim of the present study was to determine the presence and relative amounts of S. mutans and S. sobrinus in the saliva samples obtained from schoolchildren in Khartoum State, the Sudan, and to study the association of the amounts of S. mutans and S. sobrinus with caries experience, socioeconomic status and sugar-sweetened snacks in this population. 140 samples, 30 of which were from individuals with caries experience, were examined using quantitative real-time polymerase chain reaction (qRT-PCR) with specific oligonucleotide primers. The mean ratio of fold differences of S. mutans to S. sobrinus was 0.77 (SD 5.4) and 2.29 (SD 6.0) for samples obtained from caries-free and caries-active individuals, respectively. This suggested that the proportion of S. sobrinus was higher than S.mutans in the caries-active group when compared to the caries-free group. An association was found between children with caries-active lesions and the frequent consumption of sticky desserts and higher socioeconomic status. S. sobrinus seems to be associated with caries experience in the studied population. A proposal of caries screening programs designed to test for S. sobrinus in this population may be developed. SN - 1421-976X UR - https://www.unboundmedicine.com/medline/citation/20714152/Caries_experience_and_quantification_of_Streptococcus_mutans_and_Streptococcus_sobrinus_in_saliva_of_Sudanese_schoolchildren_ L2 - https://www.karger.com?DOI=10.1159/000316664 DB - PRIME DP - Unbound Medicine ER -