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Immunosuppressive activity of tilmicosin on the immune responses in mice.
Immunopharmacol Immunotoxicol. 2011 Jun; 33(2):323-8.II

Abstract

Tilmicosin, a semi-synthetic macrolide antibiotic that is only used in the veterinary clinic, was evaluated for its immunosuppressive activity on the immune responses to ovalbumin (OVA) in mice. Tilmicosin suppressed concanavalin A (Con A)- and lipopolysaccharide (LPS)-stimulated splenocyte proliferation in vitro. BALB/c mice were immunized subcutaneously with OVA on day 1 and 4. Beginning on the day of boosting immunization, the mice were administered intraperitoneally with tilmicosin at a single dose of 10, 30, and 90 mg/kg for 10 consecutive days. On day 14, blood samples were collected for measuring specific total-immunoglobulin G (total-IgG), IgG1, IgG2b, and splenocytes were harvested for determining lymphocyte proliferation and interleukin-2 (IL-2), interferon-γ (IFN-γ), IL-4 production. The results demonstrated that tilmicosin could significantly suppress Con A-induced splenocyte proliferation in a dose-dependent manner, decrease LPS-and OVA-induced splenocyte proliferation only at high concentration, produced less IL-2, IL-4, and IFN-γ as compared to the control in the OVA-immunized mice. Moreover, the OVA-specific IgG, IgG1, and IgG2b levels in the OVA-immunized mice were reduced by tilmicosin. These results suggest that tilmicosin could suppress the cellular and humoral immune response in mice.

Authors+Show Affiliations

Department of Veterinary Pharmacology, College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20718577

Citation

Guan, Shuang, et al. "Immunosuppressive Activity of Tilmicosin On the Immune Responses in Mice." Immunopharmacology and Immunotoxicology, vol. 33, no. 2, 2011, pp. 323-8.
Guan S, Song Y, Guo W, et al. Immunosuppressive activity of tilmicosin on the immune responses in mice. Immunopharmacol Immunotoxicol. 2011;33(2):323-8.
Guan, S., Song, Y., Guo, W., Chu, X., Zhang, X., Wang, D., Lu, J., & Deng, X. (2011). Immunosuppressive activity of tilmicosin on the immune responses in mice. Immunopharmacology and Immunotoxicology, 33(2), 323-8. https://doi.org/10.3109/08923973.2010.508497
Guan S, et al. Immunosuppressive Activity of Tilmicosin On the Immune Responses in Mice. Immunopharmacol Immunotoxicol. 2011;33(2):323-8. PubMed PMID: 20718577.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunosuppressive activity of tilmicosin on the immune responses in mice. AU - Guan,Shuang, AU - Song,Yu, AU - Guo,Weixiao, AU - Chu,Xiao, AU - Zhang,Xiaozhe, AU - Wang,Dacheng, AU - Lu,Jing, AU - Deng,Xuming, Y1 - 2010/08/18/ PY - 2010/8/20/entrez PY - 2010/8/20/pubmed PY - 2012/3/13/medline SP - 323 EP - 8 JF - Immunopharmacology and immunotoxicology JO - Immunopharmacol Immunotoxicol VL - 33 IS - 2 N2 - Tilmicosin, a semi-synthetic macrolide antibiotic that is only used in the veterinary clinic, was evaluated for its immunosuppressive activity on the immune responses to ovalbumin (OVA) in mice. Tilmicosin suppressed concanavalin A (Con A)- and lipopolysaccharide (LPS)-stimulated splenocyte proliferation in vitro. BALB/c mice were immunized subcutaneously with OVA on day 1 and 4. Beginning on the day of boosting immunization, the mice were administered intraperitoneally with tilmicosin at a single dose of 10, 30, and 90 mg/kg for 10 consecutive days. On day 14, blood samples were collected for measuring specific total-immunoglobulin G (total-IgG), IgG1, IgG2b, and splenocytes were harvested for determining lymphocyte proliferation and interleukin-2 (IL-2), interferon-γ (IFN-γ), IL-4 production. The results demonstrated that tilmicosin could significantly suppress Con A-induced splenocyte proliferation in a dose-dependent manner, decrease LPS-and OVA-induced splenocyte proliferation only at high concentration, produced less IL-2, IL-4, and IFN-γ as compared to the control in the OVA-immunized mice. Moreover, the OVA-specific IgG, IgG1, and IgG2b levels in the OVA-immunized mice were reduced by tilmicosin. These results suggest that tilmicosin could suppress the cellular and humoral immune response in mice. SN - 1532-2513 UR - https://www.unboundmedicine.com/medline/citation/20718577/Immunosuppressive_activity_of_tilmicosin_on_the_immune_responses_in_mice_ L2 - https://www.tandfonline.com/doi/full/10.3109/08923973.2010.508497 DB - PRIME DP - Unbound Medicine ER -