Type your tag names separated by a space and hit enter

Interaction between anandamide and sphingosine-1-phosphate in mediating vasorelaxation in rat coronary artery.

Abstract

BACKGROUND AND PURPOSE

Anandamide and sphingosine-1-phosphate (S1P) both regulate vascular tone in a variety of vessels. This study aimed to examine the mechanisms involved in the regulation of coronary vascular tone by anandamide and S1P, and to determine whether any functional interaction occurs between these receptor systems.

EXPERIMENTAL APPROACH

Mechanisms used by anandamide and S1P to regulate rat coronary artery (CA) reactivity were investigated using wire myography. Interactions between S1P and the cannabinoid (CB)(2) receptor were determined using human embryonic kidney 293 (HEK293) cells that stably over-express recombinant CB(2) receptor.

KEY RESULTS

Anandamide and S1P induced relaxation of the rat CA. CB(2) receptor antagonists attenuated anandamide-induced relaxation, while S1P-mediated relaxation was dependent on the vascular endothelium and S1P(3). Anandamide treatment resulted in an increase in the phosphorylation of sphingosine kinase-1 within the CA. Conversely, anandamide-mediated relaxation was attenuated by inhibition of sphingosine kinase. Moreover, S1P(3), specifically within the vascular endothelium, was required for anandamide-mediated vasorelaxation. In addition to this, S1P-mediated relaxation was also reduced by CB(2) receptor antagonists and sphingosine kinase inhibition. Further evidence that S1P functionally interacts with the CB(2) receptor was also observed in HEK293 cells over-expressing the CB(2) receptor.

CONCLUSIONS AND IMPLICATIONS

In the vascular endothelium of rat CA, anandamide induces relaxation via a mechanism requiring sphingosine kinase-1 and S1P/S1P(3). In addition, we report that S1P may exert some of its effects via a CB(2) receptor- and sphingosine kinase-dependent mechanism, where subsequently formed S1P may have privileged access to S1P(3) to induce vascular relaxation.

Links

  • PMC Free PDF
  • PMC Free Full Text
  • FREE Publisher Full Text
  • Authors+Show Affiliations

    ,

    Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, UK. k.mair@bio.gla.ac.uk

    , , , , ,

    Source

    British journal of pharmacology 161:1 2010 Sep pg 176-92

    MeSH

    Animals
    Arachidonic Acids
    Calcium Channel Blockers
    Cardiovascular Agents
    Cell Line
    Coronary Vessels
    Dronabinol
    Endocannabinoids
    Humans
    Indoles
    Indomethacin
    Lysophospholipids
    Male
    Polyunsaturated Alkamides
    Rats
    Rats, Sprague-Dawley
    Receptor, Cannabinoid, CB2
    Sphingosine
    Vasodilation

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    20718749

    Citation

    Mair, K M., et al. "Interaction Between Anandamide and Sphingosine-1-phosphate in Mediating Vasorelaxation in Rat Coronary Artery." British Journal of Pharmacology, vol. 161, no. 1, 2010, pp. 176-92.
    Mair KM, Robinson E, Kane KA, et al. Interaction between anandamide and sphingosine-1-phosphate in mediating vasorelaxation in rat coronary artery. Br J Pharmacol. 2010;161(1):176-92.
    Mair, K. M., Robinson, E., Kane, K. A., Pyne, S., Brett, R. R., Pyne, N. J., & Kennedy, S. (2010). Interaction between anandamide and sphingosine-1-phosphate in mediating vasorelaxation in rat coronary artery. British Journal of Pharmacology, 161(1), pp. 176-92. doi:10.1111/j.1476-5381.2010.00878.x.
    Mair KM, et al. Interaction Between Anandamide and Sphingosine-1-phosphate in Mediating Vasorelaxation in Rat Coronary Artery. Br J Pharmacol. 2010;161(1):176-92. PubMed PMID: 20718749.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Interaction between anandamide and sphingosine-1-phosphate in mediating vasorelaxation in rat coronary artery. AU - Mair,K M, AU - Robinson,E, AU - Kane,K A, AU - Pyne,S, AU - Brett,R R, AU - Pyne,N J, AU - Kennedy,S, PY - 2010/8/20/entrez PY - 2010/8/20/pubmed PY - 2010/12/29/medline SP - 176 EP - 92 JF - British journal of pharmacology JO - Br. J. Pharmacol. VL - 161 IS - 1 N2 - BACKGROUND AND PURPOSE: Anandamide and sphingosine-1-phosphate (S1P) both regulate vascular tone in a variety of vessels. This study aimed to examine the mechanisms involved in the regulation of coronary vascular tone by anandamide and S1P, and to determine whether any functional interaction occurs between these receptor systems. EXPERIMENTAL APPROACH: Mechanisms used by anandamide and S1P to regulate rat coronary artery (CA) reactivity were investigated using wire myography. Interactions between S1P and the cannabinoid (CB)(2) receptor were determined using human embryonic kidney 293 (HEK293) cells that stably over-express recombinant CB(2) receptor. KEY RESULTS: Anandamide and S1P induced relaxation of the rat CA. CB(2) receptor antagonists attenuated anandamide-induced relaxation, while S1P-mediated relaxation was dependent on the vascular endothelium and S1P(3). Anandamide treatment resulted in an increase in the phosphorylation of sphingosine kinase-1 within the CA. Conversely, anandamide-mediated relaxation was attenuated by inhibition of sphingosine kinase. Moreover, S1P(3), specifically within the vascular endothelium, was required for anandamide-mediated vasorelaxation. In addition to this, S1P-mediated relaxation was also reduced by CB(2) receptor antagonists and sphingosine kinase inhibition. Further evidence that S1P functionally interacts with the CB(2) receptor was also observed in HEK293 cells over-expressing the CB(2) receptor. CONCLUSIONS AND IMPLICATIONS: In the vascular endothelium of rat CA, anandamide induces relaxation via a mechanism requiring sphingosine kinase-1 and S1P/S1P(3). In addition, we report that S1P may exert some of its effects via a CB(2) receptor- and sphingosine kinase-dependent mechanism, where subsequently formed S1P may have privileged access to S1P(3) to induce vascular relaxation. SN - 1476-5381 UR - https://www.unboundmedicine.com/medline/citation/20718749/abstract/Interaction_between_anandamide_and_sphingosine_1_phosphate_in_mediating_vasorelaxation_in_rat_coronary_artery_ L2 - https://doi.org/10.1111/j.1476-5381.2010.00878.x DB - PRIME DP - Unbound Medicine ER -