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Optimal time point for neuronal generation of transplanted neural progenitor cells in injured spinal cord following root avulsion.
Cell Transplant. 2011; 20(2):167-76.CT

Abstract

Root avulsion of the brachial plexus results in a progressive and pronounced loss of motoneurons. Cell replacement strategies have therapeutic potential in the treatment of motoneuron degenerative neurological disorders. Here, we transplanted spinal cord-derived neural progenitor cells (NPCs) into the cervical ventral horn of adult rats immediately, 2 weeks, or 6 weeks after root avulsion to determine an optimal time scale for the survival and differentiation of grafted cells. We showed that grafted NPCs survived robustly at all three time points and there was no statistical difference in survival rate. Interestingly, however, transplantation at 2 weeks postavulsion significantly increased the neuronal differentiation of transplanted NPCs compared to transplantation immediately or at 6 weeks postavulsion. Moreover, only NPCs transplanted at 2 weeks postavulsion were able to differentiate into choline acetyltransferase (ChAT)-positive neurons. Specific ELISAs and quantitative reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated that expression levels of BDNF and GDNF were significantly upregulated in the ventral cord at 2 weeks postavulsion compared to immediately or at 6 weeks postavulsion. Our study suggests that the cervical ventral horn at 2 weeks postavulsion both supports neuronal differentiation and induces region-specific neuronal generation possibly because of its higher expression of BDNF and GDNF.

Authors+Show Affiliations

Department of Anatomy, The University of Hong Kong, Pokfulam, Hong Kong SAR, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20719091

Citation

Su, Huanxing, et al. "Optimal Time Point for Neuronal Generation of Transplanted Neural Progenitor Cells in Injured Spinal Cord Following Root Avulsion." Cell Transplantation, vol. 20, no. 2, 2011, pp. 167-76.
Su H, Wu Y, Yuan Q, et al. Optimal time point for neuronal generation of transplanted neural progenitor cells in injured spinal cord following root avulsion. Cell Transplant. 2011;20(2):167-76.
Su, H., Wu, Y., Yuan, Q., Guo, J., Zhang, W., & Wu, W. (2011). Optimal time point for neuronal generation of transplanted neural progenitor cells in injured spinal cord following root avulsion. Cell Transplantation, 20(2), 167-76. https://doi.org/10.3727/096368910X522090
Su H, et al. Optimal Time Point for Neuronal Generation of Transplanted Neural Progenitor Cells in Injured Spinal Cord Following Root Avulsion. Cell Transplant. 2011;20(2):167-76. PubMed PMID: 20719091.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimal time point for neuronal generation of transplanted neural progenitor cells in injured spinal cord following root avulsion. AU - Su,Huanxing, AU - Wu,Yin, AU - Yuan,Qiuju, AU - Guo,Jiasong, AU - Zhang,Wenming, AU - Wu,Wutian, Y1 - 2010/08/18/ PY - 2010/8/20/entrez PY - 2010/8/20/pubmed PY - 2011/8/24/medline SP - 167 EP - 76 JF - Cell transplantation JO - Cell Transplant VL - 20 IS - 2 N2 - Root avulsion of the brachial plexus results in a progressive and pronounced loss of motoneurons. Cell replacement strategies have therapeutic potential in the treatment of motoneuron degenerative neurological disorders. Here, we transplanted spinal cord-derived neural progenitor cells (NPCs) into the cervical ventral horn of adult rats immediately, 2 weeks, or 6 weeks after root avulsion to determine an optimal time scale for the survival and differentiation of grafted cells. We showed that grafted NPCs survived robustly at all three time points and there was no statistical difference in survival rate. Interestingly, however, transplantation at 2 weeks postavulsion significantly increased the neuronal differentiation of transplanted NPCs compared to transplantation immediately or at 6 weeks postavulsion. Moreover, only NPCs transplanted at 2 weeks postavulsion were able to differentiate into choline acetyltransferase (ChAT)-positive neurons. Specific ELISAs and quantitative reverse transcriptase polymerase chain reaction (RT-PCR) demonstrated that expression levels of BDNF and GDNF were significantly upregulated in the ventral cord at 2 weeks postavulsion compared to immediately or at 6 weeks postavulsion. Our study suggests that the cervical ventral horn at 2 weeks postavulsion both supports neuronal differentiation and induces region-specific neuronal generation possibly because of its higher expression of BDNF and GDNF. SN - 1555-3892 UR - https://www.unboundmedicine.com/medline/citation/20719091/Optimal_time_point_for_neuronal_generation_of_transplanted_neural_progenitor_cells_in_injured_spinal_cord_following_root_avulsion_ L2 - http://journals.sagepub.com/doi/full/10.3727/096368910X522090?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -