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Analysis of biological samples by capillary electrophoresis with laser induced fluorescence detection.
J Pharm Biomed Anal. 2010 Dec 15; 53(5):1180-92.JP

Abstract

In this paper an overview is provided on practical difficulties as well as applications of capillary electrophoresis coupled to laser induced fluorescence detection methods in the field of analysis of biological samples. Various methodological approaches elaborated for determination of small molecules, peptides and proteins are outlined. Besides giving an overview on detection based on native fluorescence, immune and enzyme assays, the main focus is the problematics of sample derivatization and achievable detection sensitivities in the analysis of real biological samples. The characteristics and applicability of the most commonly used labeling reagents are discussed in details.

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Authors+Show Affiliations

Szöko E
Department of Pharmacodynamics, Semmelweis University, Nagyvárad tér 4, H-1089 Budapest, Hungary. eva.szoko@net.sote.hu
Tábi T
No affiliation info available

MeSH

AnimalsBiological ProductsChemistry Techniques, AnalyticalElectrophoresis, CapillaryHumansLasersSpectrometry, Fluorescence

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Review

Language

eng

PubMed ID

20719451

Citation

Szöko, Eva, and Tamás Tábi. "Analysis of Biological Samples By Capillary Electrophoresis With Laser Induced Fluorescence Detection." Journal of Pharmaceutical and Biomedical Analysis, vol. 53, no. 5, 2010, pp. 1180-92.
Szöko E, Tábi T. Analysis of biological samples by capillary electrophoresis with laser induced fluorescence detection. J Pharm Biomed Anal. 2010;53(5):1180-92.
Szöko, E., & Tábi, T. (2010). Analysis of biological samples by capillary electrophoresis with laser induced fluorescence detection. Journal of Pharmaceutical and Biomedical Analysis, 53(5), 1180-92. https://doi.org/10.1016/j.jpba.2010.07.045
Szöko E, Tábi T. Analysis of Biological Samples By Capillary Electrophoresis With Laser Induced Fluorescence Detection. J Pharm Biomed Anal. 2010 Dec 15;53(5):1180-92. PubMed PMID: 20719451.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of biological samples by capillary electrophoresis with laser induced fluorescence detection. AU - Szöko,Eva, AU - Tábi,Tamás, Y1 - 2010/08/06/ PY - 2010/05/20/received PY - 2010/07/23/revised PY - 2010/07/27/accepted PY - 2010/8/20/entrez PY - 2010/8/20/pubmed PY - 2011/6/2/medline SP - 1180 EP - 92 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 53 IS - 5 N2 - In this paper an overview is provided on practical difficulties as well as applications of capillary electrophoresis coupled to laser induced fluorescence detection methods in the field of analysis of biological samples. Various methodological approaches elaborated for determination of small molecules, peptides and proteins are outlined. Besides giving an overview on detection based on native fluorescence, immune and enzyme assays, the main focus is the problematics of sample derivatization and achievable detection sensitivities in the analysis of real biological samples. The characteristics and applicability of the most commonly used labeling reagents are discussed in details. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/20719451/Analysis_of_biological_samples_by_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(10)00435-8 DB - PRIME DP - Unbound Medicine ER -