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Isolation and identification of antioxidant compounds from Ligularia fischeri.
J Food Sci. 2010 Aug 01; 75(6):C530-5.JF

Abstract

The plant Ligularia fischeri var. spiciformis Nakai, a well-known edible medicinal herb in Korea, has been used to treat maladies such as jaundice, scarlet fever, rheumatoid arthritis, and hepatic function failure. In this research, 4 major antioxidant compounds were detected from this plant's leaves using an on-line high-performance liquid chromatography (HPLC)-ABTS screening system, which can determine the antioxidant activity based on a decrease in absorbance at 734 nm after postcolumn reaction of HPLC-separated antioxidants with the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radicals (ABTS(*)). In order to isolate these active compounds, a preparative HPLC was applied and their chemical structures were identified as 5-O-caffeoylquinic acid (5-CQA), 3,4-di-O-caffeoylquinic acid (3,4-DCQA), 3,5-di-O-caffeoylquinic acid (3,5-DCQA), and 4,5-di-O-caffeoylquinic acid (4,5-DCQA) by ESI/MS(n) and (1)H NMR. These 4 isomers comprised over 10% of the dried leaves, with 3,5-DCQA being the most abundant compound. The radical scavenging activity of each isomer was also evaluated simultaneously through the on-line HPLC-ABTS method, which showed 94% antioxidant activity of the ethanol extract derived from caffeoylquinic acids. Among these isomers, 3,4-DCQA contained the most strong antioxidant activity while 3,5-DCQA accounted for the highest radical scavenging capacity due to having the highest content.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Shang YF
Natural Product Research Center, KIST Gangneung Inst., Daejeon-dong, Gangneung, Gangwon, Republic of Korea.
Kim SM
No affiliation info available
Song DG
No affiliation info available
Pan CH
No affiliation info available
Lee WJ
No affiliation info available
Um BH
No affiliation info available

MeSH

AntioxidantsAsteraceaeBenzothiazolesChlorogenic AcidChromatography, High Pressure LiquidDrug DiscoveryIndicators and ReagentsMagnetic Resonance SpectroscopyMedicine, Korean TraditionalMolecular StructureMonosaccharidesPlant ExtractsPlant LeavesQuinic AcidSpectrometry, Mass, Electrospray IonizationSulfonic AcidsTandem Mass Spectrometry

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20722907

Citation

Shang, Ya Fang, et al. "Isolation and Identification of Antioxidant Compounds From Ligularia Fischeri." Journal of Food Science, vol. 75, no. 6, 2010, pp. C530-5.
Shang YF, Kim SM, Song DG, et al. Isolation and identification of antioxidant compounds from Ligularia fischeri. J Food Sci. 2010;75(6):C530-5.
Shang, Y. F., Kim, S. M., Song, D. G., Pan, C. H., Lee, W. J., & Um, B. H. (2010). Isolation and identification of antioxidant compounds from Ligularia fischeri. Journal of Food Science, 75(6), C530-5. https://doi.org/10.1111/j.1750-3841.2010.01714.x
Shang YF, et al. Isolation and Identification of Antioxidant Compounds From Ligularia Fischeri. J Food Sci. 2010 Aug 1;75(6):C530-5. PubMed PMID: 20722907.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Isolation and identification of antioxidant compounds from Ligularia fischeri. AU - Shang,Ya Fang, AU - Kim,Sang Min, AU - Song,Dae-Geun, AU - Pan,Cheol-Ho, AU - Lee,Won Jong, AU - Um,Byung-Hun, PY - 2010/8/21/entrez PY - 2010/8/21/pubmed PY - 2011/1/19/medline SP - C530 EP - 5 JF - Journal of food science JO - J Food Sci VL - 75 IS - 6 N2 - The plant Ligularia fischeri var. spiciformis Nakai, a well-known edible medicinal herb in Korea, has been used to treat maladies such as jaundice, scarlet fever, rheumatoid arthritis, and hepatic function failure. In this research, 4 major antioxidant compounds were detected from this plant's leaves using an on-line high-performance liquid chromatography (HPLC)-ABTS screening system, which can determine the antioxidant activity based on a decrease in absorbance at 734 nm after postcolumn reaction of HPLC-separated antioxidants with the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) radicals (ABTS(*)). In order to isolate these active compounds, a preparative HPLC was applied and their chemical structures were identified as 5-O-caffeoylquinic acid (5-CQA), 3,4-di-O-caffeoylquinic acid (3,4-DCQA), 3,5-di-O-caffeoylquinic acid (3,5-DCQA), and 4,5-di-O-caffeoylquinic acid (4,5-DCQA) by ESI/MS(n) and (1)H NMR. These 4 isomers comprised over 10% of the dried leaves, with 3,5-DCQA being the most abundant compound. The radical scavenging activity of each isomer was also evaluated simultaneously through the on-line HPLC-ABTS method, which showed 94% antioxidant activity of the ethanol extract derived from caffeoylquinic acids. Among these isomers, 3,4-DCQA contained the most strong antioxidant activity while 3,5-DCQA accounted for the highest radical scavenging capacity due to having the highest content. SN - 1750-3841 UR - https://www.unboundmedicine.com/medline/citation/20722907/Isolation_and_identification_of_antioxidant_compounds_from_Ligularia_fischeri_ DB - PRIME DP - Unbound Medicine ER -