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Proteomic identification of proteins involved in the anticancer activities of oridonin in HepG2 cells.
Phytomedicine. 2011 Jan 15; 18(2-3):163-9.P

Abstract

Oridonin is the main bioactive constituent of the Chinese medicinal herb Isodon rubescens and has been shown to have anti-neoplastic effects against a number of cancers in vitro and in vivo. Here we report the proteomic identification of proteins involved in the anticancer properties of oridonin in hepatocarcinoma HepG2 cells. Cell viability assay showed that oridonin dose-dependently inhibited cell growth with an IC(50) of 41.77μM. Treatment with oridonin at 44μM for 24h induced apoptosis and G2/M cell cycle arrest, which were associated with nine differentially expressed proteins identified by proteomic analysis. The proteomic expression patterns of Hsp70.1, Sti1 and hnRNP-E1 were confirmed by quantitative real-time PCR and/or immunoblotting. Eight of the nine identified proteins are shown, for the first time, to be involved in the anticancer activities of oridonin. Up-regulation of Hsp70.1, STRAP, TCTP, Sti1 and PPase, as well as the down-regulation of hnRNP-E1 could be responsible for the apoptotic and G2/M-arresting effects of oridonin observed in this study. Up-regulation of HP1 beta and GlyRS might contribute to inhibitory effects of oridonin on telomerase and tyrosine kinase, respectively. These findings shed new insights into the molecular mechanisms underlying the anticancer properties of oridonin in liver cancer cells.

Authors+Show Affiliations

Center for Cancer and Inflammation Research, School of Chinese Medicine, Hong Kong Baptist University, Kowloon Tong, Hong Kong, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20724128

Citation

Wang, Hui, et al. "Proteomic Identification of Proteins Involved in the Anticancer Activities of Oridonin in HepG2 Cells." Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, vol. 18, no. 2-3, 2011, pp. 163-9.
Wang H, Ye Y, Pan SY, et al. Proteomic identification of proteins involved in the anticancer activities of oridonin in HepG2 cells. Phytomedicine. 2011;18(2-3):163-9.
Wang, H., Ye, Y., Pan, S. Y., Zhu, G. Y., Li, Y. W., Fong, D. W., & Yu, Z. L. (2011). Proteomic identification of proteins involved in the anticancer activities of oridonin in HepG2 cells. Phytomedicine : International Journal of Phytotherapy and Phytopharmacology, 18(2-3), 163-9. https://doi.org/10.1016/j.phymed.2010.06.011
Wang H, et al. Proteomic Identification of Proteins Involved in the Anticancer Activities of Oridonin in HepG2 Cells. Phytomedicine. 2011 Jan 15;18(2-3):163-9. PubMed PMID: 20724128.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteomic identification of proteins involved in the anticancer activities of oridonin in HepG2 cells. AU - Wang,Hui, AU - Ye,Yan, AU - Pan,Si-Yuan, AU - Zhu,Guo-Yuan, AU - Li,Ying-Wei, AU - Fong,David W F, AU - Yu,Zhi-Ling, Y1 - 2010/08/17/ PY - 2009/10/28/received PY - 2010/05/03/revised PY - 2010/06/11/accepted PY - 2010/8/21/entrez PY - 2010/8/21/pubmed PY - 2011/8/9/medline SP - 163 EP - 9 JF - Phytomedicine : international journal of phytotherapy and phytopharmacology JO - Phytomedicine VL - 18 IS - 2-3 N2 - Oridonin is the main bioactive constituent of the Chinese medicinal herb Isodon rubescens and has been shown to have anti-neoplastic effects against a number of cancers in vitro and in vivo. Here we report the proteomic identification of proteins involved in the anticancer properties of oridonin in hepatocarcinoma HepG2 cells. Cell viability assay showed that oridonin dose-dependently inhibited cell growth with an IC(50) of 41.77μM. Treatment with oridonin at 44μM for 24h induced apoptosis and G2/M cell cycle arrest, which were associated with nine differentially expressed proteins identified by proteomic analysis. The proteomic expression patterns of Hsp70.1, Sti1 and hnRNP-E1 were confirmed by quantitative real-time PCR and/or immunoblotting. Eight of the nine identified proteins are shown, for the first time, to be involved in the anticancer activities of oridonin. Up-regulation of Hsp70.1, STRAP, TCTP, Sti1 and PPase, as well as the down-regulation of hnRNP-E1 could be responsible for the apoptotic and G2/M-arresting effects of oridonin observed in this study. Up-regulation of HP1 beta and GlyRS might contribute to inhibitory effects of oridonin on telomerase and tyrosine kinase, respectively. These findings shed new insights into the molecular mechanisms underlying the anticancer properties of oridonin in liver cancer cells. SN - 1618-095X UR - https://www.unboundmedicine.com/medline/citation/20724128/Proteomic_identification_of_proteins_involved_in_the_anticancer_activities_of_oridonin_in_HepG2_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0944-7113(10)00215-1 DB - PRIME DP - Unbound Medicine ER -