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Effects of streptomycin on growth of algae Chlorella vulgaris and Microcystis aeruginosa.
Environ Toxicol. 2012 Mar; 27(4):229-37.ET

Abstract

Streptomycin is a common contaminant in a variety of industrial and agricultural wastewaters. The available information on the potential toxicity of streptomycin of fresh algae implicated in the treatment of biological wastewater is extremely limited. The objective of this study was to evaluate the effects of streptomycin on physiological indices and photosynthesis-related gene transcription. The results of short-term batch bioassays indicated that streptomycin was more sensitive to cyanobacteria than to green algae. The EC50 of streptomycin in Microcystis aeruginosa and Chlorella vulgaris were 0.28 and 20.08 mg L(-1) , respectively. These selected streptomycin concentrations inhibited algal cell growth and decreased chlorophyll or phycocyanobilin content. Streptomycin also destroyed the overall membrane system, which was speculated from malondialdehyde (MDA) content and electrolyte leakage increasing after streptomycin exposure. Two algae were induced to increase their antioxidant enzyme activities to withstand streptomycin. However, the balance between oxidant substance and antioxidant enzyme was broken, because reactive oxygen species (ROS) content simultaneously increased. Streptomycin inhibited photosynthesis-related gene transcription in C. vulgaris and M. aeruginosa. Transcript levels of psaB, psbA, and rbcL in C. vulgaris decreased to only 14.5%, 32.2%, and 9.3% of the control, respectively. Similarly, the transcript levels of psaB, psbD, and rbcL in M. aeruginosa decreased markedly in the present of streptomycin. The transcription of these genes was 12.4%, 26.1%, and 28.4% of the control after 0.1 mg L(-1) streptomycin exposure, respectively. Our results demonstrate that streptomycin is toxic to fresh algae, affects photosynthesis-related gene transcription, and blocks electron transport and ROS overproduction.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Qian H
Department of Biotechnology, College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, People's Republic of China.
Li J
No affiliation info available
Pan X
No affiliation info available
Sun Z
No affiliation info available
Ye C
No affiliation info available
Jin G
No affiliation info available
Fu Z
No affiliation info available

MeSH

Algal ProteinsAnti-Bacterial AgentsChlorella vulgarisChlorophyllElectron TransportMalondialdehydeMicrocystisOxidation-ReductionPhotosynthesisReactive Oxygen SpeciesStreptomycinTranscription, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20725941

Citation

Qian, Haifeng, et al. "Effects of Streptomycin On Growth of Algae Chlorella Vulgaris and Microcystis Aeruginosa." Environmental Toxicology, vol. 27, no. 4, 2012, pp. 229-37.
Qian H, Li J, Pan X, et al. Effects of streptomycin on growth of algae Chlorella vulgaris and Microcystis aeruginosa. Environ Toxicol. 2012;27(4):229-37.
Qian, H., Li, J., Pan, X., Sun, Z., Ye, C., Jin, G., & Fu, Z. (2012). Effects of streptomycin on growth of algae Chlorella vulgaris and Microcystis aeruginosa. Environmental Toxicology, 27(4), 229-37. https://doi.org/10.1002/tox.20636
Qian H, et al. Effects of Streptomycin On Growth of Algae Chlorella Vulgaris and Microcystis Aeruginosa. Environ Toxicol. 2012;27(4):229-37. PubMed PMID: 20725941.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of streptomycin on growth of algae Chlorella vulgaris and Microcystis aeruginosa. AU - Qian,Haifeng, AU - Li,Jingjing, AU - Pan,Xiangjie, AU - Sun,Zhengqi, AU - Ye,Chengbin, AU - Jin,Gongqin, AU - Fu,Zhengwei, Y1 - 2010/08/19/ PY - 2010/03/09/received PY - 2010/06/06/revised PY - 2010/06/15/accepted PY - 2010/8/21/entrez PY - 2010/8/21/pubmed PY - 2012/8/15/medline SP - 229 EP - 37 JF - Environmental toxicology JO - Environ Toxicol VL - 27 IS - 4 N2 - Streptomycin is a common contaminant in a variety of industrial and agricultural wastewaters. The available information on the potential toxicity of streptomycin of fresh algae implicated in the treatment of biological wastewater is extremely limited. The objective of this study was to evaluate the effects of streptomycin on physiological indices and photosynthesis-related gene transcription. The results of short-term batch bioassays indicated that streptomycin was more sensitive to cyanobacteria than to green algae. The EC50 of streptomycin in Microcystis aeruginosa and Chlorella vulgaris were 0.28 and 20.08 mg L(-1) , respectively. These selected streptomycin concentrations inhibited algal cell growth and decreased chlorophyll or phycocyanobilin content. Streptomycin also destroyed the overall membrane system, which was speculated from malondialdehyde (MDA) content and electrolyte leakage increasing after streptomycin exposure. Two algae were induced to increase their antioxidant enzyme activities to withstand streptomycin. However, the balance between oxidant substance and antioxidant enzyme was broken, because reactive oxygen species (ROS) content simultaneously increased. Streptomycin inhibited photosynthesis-related gene transcription in C. vulgaris and M. aeruginosa. Transcript levels of psaB, psbA, and rbcL in C. vulgaris decreased to only 14.5%, 32.2%, and 9.3% of the control, respectively. Similarly, the transcript levels of psaB, psbD, and rbcL in M. aeruginosa decreased markedly in the present of streptomycin. The transcription of these genes was 12.4%, 26.1%, and 28.4% of the control after 0.1 mg L(-1) streptomycin exposure, respectively. Our results demonstrate that streptomycin is toxic to fresh algae, affects photosynthesis-related gene transcription, and blocks electron transport and ROS overproduction. SN - 1522-7278 UR - https://www.unboundmedicine.com/medline/citation/20725941/Effects_of_streptomycin_on_growth_of_algae_Chlorella_vulgaris_and_Microcystis_aeruginosa_ L2 - https://doi.org/10.1002/tox.20636 DB - PRIME DP - Unbound Medicine ER -