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Dose response effect of rutin a dietary antioxidant on alcohol-induced prooxidant and antioxidant imbalance - a histopathologic study.

Abstract

The present study was designed to investigate the effect of rutin on ethanol-induced hepatotoxicity in a dose-dependent manner in rats. Male albino rats were divided into six groups. Group 1 rats served as control and group 2 rats received rutin 100 mg/kg body weight. Hepatotoxicity was induced in groups 3-6 rats (20% ethanol) for 60 days. In addition, groups 4-6 rats received rutin at doses of 25, 50, 100 mg/kg body weight, respectively for the last 30 days of the experiment. We observed a significant increase in the activities of liver marker enzymes, serum amino transferases, alkaline phosphatase, γ-glutamyl transpeptidase the levels of thiobarbituric acid reactive substances, conjugated dienes, lipid hydroperoxides, and a decrease in the activities of superoxide dismutase (SOD), catalase (CAT), glutathione and its related enzymes, vitamins C and E when compared to ethanol-fed rats. Rutin supplementation along with ethanol significantly decreased the levels of liver marker enzymes, lipid peroxidation and significantly elevated the activities of liver SOD, CAT, GSH, glutathione peroxidase, vitamins C and E when compared to untreated ethanol supplemented rats. Among the three doses, 100 mg/kg body weight of rutin was found to exert a more pronounced hepatoprotective effect against ethanol-induced toxicity. Our results were also confirmed by the histopathologic observations.

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  • Authors+Show Affiliations

    ,

    Faculty of Science, Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar 608 002, Tamilnadu, India.

    Source

    Fundamental & clinical pharmacology 25:4 2011 Aug pg 493-502

    MeSH

    Administration, Oral
    Alanine Transaminase
    Alkadienes
    Alkaline Phosphatase
    Animals
    Antioxidants
    Ascorbic Acid
    Aspartate Aminotransferases
    Body Weight
    Catalase
    Dose-Response Relationship, Drug
    Ethanol
    Glutathione
    Glutathione Peroxidase
    Glutathione Transferase
    Lipid Peroxides
    Liver
    Liver Diseases, Alcoholic
    Male
    Oxidative Stress
    Rats
    Rats, Wistar
    Rutin
    Superoxide Dismutase
    Thiobarbituric Acid Reactive Substances
    Vitamin E
    gamma-Glutamyltransferase

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    20727014

    Citation

    Shenbagam, Madhavan, and Namasivayam Nalini. "Dose Response Effect of Rutin a Dietary Antioxidant On Alcohol-induced Prooxidant and Antioxidant Imbalance - a Histopathologic Study." Fundamental & Clinical Pharmacology, vol. 25, no. 4, 2011, pp. 493-502.
    Shenbagam M, Nalini N. Dose response effect of rutin a dietary antioxidant on alcohol-induced prooxidant and antioxidant imbalance - a histopathologic study. Fundam Clin Pharmacol. 2011;25(4):493-502.
    Shenbagam, M., & Nalini, N. (2011). Dose response effect of rutin a dietary antioxidant on alcohol-induced prooxidant and antioxidant imbalance - a histopathologic study. Fundamental & Clinical Pharmacology, 25(4), pp. 493-502. doi:10.1111/j.1472-8206.2010.00861.x.
    Shenbagam M, Nalini N. Dose Response Effect of Rutin a Dietary Antioxidant On Alcohol-induced Prooxidant and Antioxidant Imbalance - a Histopathologic Study. Fundam Clin Pharmacol. 2011;25(4):493-502. PubMed PMID: 20727014.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Dose response effect of rutin a dietary antioxidant on alcohol-induced prooxidant and antioxidant imbalance - a histopathologic study. AU - Shenbagam,Madhavan, AU - Nalini,Namasivayam, Y1 - 2010/08/19/ PY - 2010/8/24/entrez PY - 2010/8/24/pubmed PY - 2012/1/25/medline SP - 493 EP - 502 JF - Fundamental & clinical pharmacology JO - Fundam Clin Pharmacol VL - 25 IS - 4 N2 - The present study was designed to investigate the effect of rutin on ethanol-induced hepatotoxicity in a dose-dependent manner in rats. Male albino rats were divided into six groups. Group 1 rats served as control and group 2 rats received rutin 100 mg/kg body weight. Hepatotoxicity was induced in groups 3-6 rats (20% ethanol) for 60 days. In addition, groups 4-6 rats received rutin at doses of 25, 50, 100 mg/kg body weight, respectively for the last 30 days of the experiment. We observed a significant increase in the activities of liver marker enzymes, serum amino transferases, alkaline phosphatase, γ-glutamyl transpeptidase the levels of thiobarbituric acid reactive substances, conjugated dienes, lipid hydroperoxides, and a decrease in the activities of superoxide dismutase (SOD), catalase (CAT), glutathione and its related enzymes, vitamins C and E when compared to ethanol-fed rats. Rutin supplementation along with ethanol significantly decreased the levels of liver marker enzymes, lipid peroxidation and significantly elevated the activities of liver SOD, CAT, GSH, glutathione peroxidase, vitamins C and E when compared to untreated ethanol supplemented rats. Among the three doses, 100 mg/kg body weight of rutin was found to exert a more pronounced hepatoprotective effect against ethanol-induced toxicity. Our results were also confirmed by the histopathologic observations. SN - 1472-8206 UR - https://www.unboundmedicine.com/medline/citation/20727014/Dose_response_effect_of_rutin_a_dietary_antioxidant_on_alcohol_induced_prooxidant_and_antioxidant_imbalance___a_histopathologic_study_ L2 - https://doi.org/10.1111/j.1472-8206.2010.00861.x DB - PRIME DP - Unbound Medicine ER -