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Kinetics of dengue virus NS1 protein in dengue 4-confirmed adult patients.
Diagn Microbiol Infect Dis. 2010 Sep; 68(1):46-9.DM

Abstract

In this work, the presence of NS1 protein as a possible early marker of dengue infection was studied in serum samples from confirmed adult patients with a primary and secondary dengue 4 infection. A total of 209 serum samples collected from day 2 up to day 7 of fever onset from 71 patients were tested by Platelia NS1 antigen capture ELISA kit (BioRad, Marnes-la-Coquette, France), and the results were compared with those obtained by capture antidengue virus IgM (MAC)-ELISA and ELISA inhibition method tests. The 83.3% of primary cases and 96.4% of secondary cases were NS1 positive. The kinetics of NS1 protein showed the highest values in optical density mean ratio or in percentage of positives between days 2 and 4. The results obtained in this study show the utility of the NS1 protein as a virologic early marker of dengue infection. Prospective studies should be carried out to confirm its utility as a prognostic marker of severe illness.

Authors+Show Affiliations

Pedro Kourí Tropical Medicine Institute (IPK), PAHO/WHO Collaborating Center for the Study of Dengue and its Vector, Havana City, Cuba. svazquez@ipk.sld.cuNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20727469

Citation

Vazquez, Susana, et al. "Kinetics of Dengue Virus NS1 Protein in Dengue 4-confirmed Adult Patients." Diagnostic Microbiology and Infectious Disease, vol. 68, no. 1, 2010, pp. 46-9.
Vazquez S, Ruiz D, Barrero R, et al. Kinetics of dengue virus NS1 protein in dengue 4-confirmed adult patients. Diagn Microbiol Infect Dis. 2010;68(1):46-9.
Vazquez, S., Ruiz, D., Barrero, R., Ramirez, R., Calzada, N., del Rosario Peña, B., Reyes, S., & Guzman, M. G. (2010). Kinetics of dengue virus NS1 protein in dengue 4-confirmed adult patients. Diagnostic Microbiology and Infectious Disease, 68(1), 46-9. https://doi.org/10.1016/j.diagmicrobio.2010.04.006
Vazquez S, et al. Kinetics of Dengue Virus NS1 Protein in Dengue 4-confirmed Adult Patients. Diagn Microbiol Infect Dis. 2010;68(1):46-9. PubMed PMID: 20727469.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Kinetics of dengue virus NS1 protein in dengue 4-confirmed adult patients. AU - Vazquez,Susana, AU - Ruiz,Didye, AU - Barrero,Rafael, AU - Ramirez,Rosa, AU - Calzada,Naifi, AU - del Rosario Peña,Blanca, AU - Reyes,Silvia, AU - Guzman,María Guadalupe, PY - 2010/02/05/received PY - 2010/03/31/revised PY - 2010/04/09/accepted PY - 2010/8/24/entrez PY - 2010/8/24/pubmed PY - 2010/12/17/medline SP - 46 EP - 9 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 68 IS - 1 N2 - In this work, the presence of NS1 protein as a possible early marker of dengue infection was studied in serum samples from confirmed adult patients with a primary and secondary dengue 4 infection. A total of 209 serum samples collected from day 2 up to day 7 of fever onset from 71 patients were tested by Platelia NS1 antigen capture ELISA kit (BioRad, Marnes-la-Coquette, France), and the results were compared with those obtained by capture antidengue virus IgM (MAC)-ELISA and ELISA inhibition method tests. The 83.3% of primary cases and 96.4% of secondary cases were NS1 positive. The kinetics of NS1 protein showed the highest values in optical density mean ratio or in percentage of positives between days 2 and 4. The results obtained in this study show the utility of the NS1 protein as a virologic early marker of dengue infection. Prospective studies should be carried out to confirm its utility as a prognostic marker of severe illness. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/20727469/Kinetics_of_dengue_virus_NS1_protein_in_dengue_4_confirmed_adult_patients_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0732-8893(10)00129-X DB - PRIME DP - Unbound Medicine ER -