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Trichomonas vaginalis transcription-mediated amplification-based analyte-specific reagent and alternative target testing of primary clinical vaginal saline suspensions.
Diagn Microbiol Infect Dis. 2010 Sep; 68(1):66-72.DM

Abstract

Following wet mount analysis, 255 vaginal saline suspensions were aliquoted to lysis medium for transcription-mediated amplification (TMA)-based Trichomonas vaginalis analyte-specific reagent testing (ASR) (Gen-Probe, San Diego, CA). Specimens with visible T. vaginalis were then refrigerated, with additional aliquoting at later intervals. Twenty-four wet mount-positive specimens (9.4%) yielded a median luminescent value (x1000, relative light unit [RLU]) of 4736. In contrast, RLU ranged from 1 to 21 following ASR of 204 wet mount-negative specimens. Twenty-seven wet mount-negative specimens (10.5%) were positive by ASR and subsequently positive via T. vaginalis alternative target TMA (Gen-Probe). Discrepancies were additionally resolved by demonstration of T. vaginalis nucleic acid from a separate endocervical collection. T. vaginalis nucleic acid was detectable following prolonged storage, following minimal incubation in lysis medium, and from low-volume aliquots of sparsely populated specimens. T. vaginalis ASR adequately detects T. vaginalis from vaginal saline suspension aliquots, providing a simple specimen alternative for a highly sensitive laboratory diagnosis of trichomoniasis.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Munson E
Wheaton Franciscan Laboratory, Wauwatosa, WI 53226, USA. erik.munson@wfhc.org
Napierala M
No affiliation info available
Basile J
No affiliation info available
Miller C
No affiliation info available
Burtch J
No affiliation info available
Hryciuk JE
No affiliation info available
Schell RF
No affiliation info available

MeSH

AnimalsFemaleHumansNucleic Acid Amplification TechniquesSodium ChlorideSpecimen HandlingTranscription, GeneticTrichomonas VaginitisTrichomonas vaginalisVagina

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

20727473

Citation

Munson, Erik, et al. "Trichomonas Vaginalis Transcription-mediated Amplification-based Analyte-specific Reagent and Alternative Target Testing of Primary Clinical Vaginal Saline Suspensions." Diagnostic Microbiology and Infectious Disease, vol. 68, no. 1, 2010, pp. 66-72.
Munson E, Napierala M, Basile J, et al. Trichomonas vaginalis transcription-mediated amplification-based analyte-specific reagent and alternative target testing of primary clinical vaginal saline suspensions. Diagn Microbiol Infect Dis. 2010;68(1):66-72.
Munson, E., Napierala, M., Basile, J., Miller, C., Burtch, J., Hryciuk, J. E., & Schell, R. F. (2010). Trichomonas vaginalis transcription-mediated amplification-based analyte-specific reagent and alternative target testing of primary clinical vaginal saline suspensions. Diagnostic Microbiology and Infectious Disease, 68(1), 66-72. https://doi.org/10.1016/j.diagmicrobio.2010.05.002
Munson E, et al. Trichomonas Vaginalis Transcription-mediated Amplification-based Analyte-specific Reagent and Alternative Target Testing of Primary Clinical Vaginal Saline Suspensions. Diagn Microbiol Infect Dis. 2010;68(1):66-72. PubMed PMID: 20727473.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Trichomonas vaginalis transcription-mediated amplification-based analyte-specific reagent and alternative target testing of primary clinical vaginal saline suspensions. AU - Munson,Erik, AU - Napierala,Maureen, AU - Basile,Janice, AU - Miller,Cheryl, AU - Burtch,Jason, AU - Hryciuk,Jeanne E, AU - Schell,Ronald F, PY - 2010/03/08/received PY - 2010/04/23/revised PY - 2010/05/03/accepted PY - 2010/8/24/entrez PY - 2010/8/24/pubmed PY - 2010/12/17/medline SP - 66 EP - 72 JF - Diagnostic microbiology and infectious disease JO - Diagn Microbiol Infect Dis VL - 68 IS - 1 N2 - Following wet mount analysis, 255 vaginal saline suspensions were aliquoted to lysis medium for transcription-mediated amplification (TMA)-based Trichomonas vaginalis analyte-specific reagent testing (ASR) (Gen-Probe, San Diego, CA). Specimens with visible T. vaginalis were then refrigerated, with additional aliquoting at later intervals. Twenty-four wet mount-positive specimens (9.4%) yielded a median luminescent value (x1000, relative light unit [RLU]) of 4736. In contrast, RLU ranged from 1 to 21 following ASR of 204 wet mount-negative specimens. Twenty-seven wet mount-negative specimens (10.5%) were positive by ASR and subsequently positive via T. vaginalis alternative target TMA (Gen-Probe). Discrepancies were additionally resolved by demonstration of T. vaginalis nucleic acid from a separate endocervical collection. T. vaginalis nucleic acid was detectable following prolonged storage, following minimal incubation in lysis medium, and from low-volume aliquots of sparsely populated specimens. T. vaginalis ASR adequately detects T. vaginalis from vaginal saline suspension aliquots, providing a simple specimen alternative for a highly sensitive laboratory diagnosis of trichomoniasis. SN - 1879-0070 UR - https://www.unboundmedicine.com/medline/citation/20727473/Trichomonas_vaginalis_transcription_mediated_amplification_based_analyte_specific_reagent_and_alternative_target_testing_of_primary_clinical_vaginal_saline_suspensions_ DB - PRIME DP - Unbound Medicine ER -
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