Tags

Type your tag names separated by a space and hit enter

Optical coherence tomography is a valuable tool in the study of the effects of microneedle geometry on skin penetration characteristics and in-skin dissolution.
J Control Release 2010; 147(3):333-41JC

Abstract

In this study, we used optical coherence tomography (OCT) to extensively investigate, for the first time, the effect that microneedle (MN) geometry (MN height, and MN interspacing) and force of application have upon penetration characteristics of soluble poly(methylvinylether-co-maleic anhydride, PMVE/MA) MN arrays into neonatal porcine skin in vitro. The results from OCT investigations were then used to design optimal and suboptimal MN-based drug delivery systems and evaluate their drug delivery profiles cross full thickness and dermatomed neonatal porcine skin in vitro. It was found that increasing the force used for MN application resulted in a significant increase in the depth of penetration achieved within neonatal porcine skin. For example, MN of 600μm height penetrated to a depth of 330μm when inserted at a force of 4.4N/array, while the penetration increased significantly to a depth of 520μm, when the force of application was increased to 16.4N/array. At an application force of 11.0N/array it was found that, in each case, increasing MN height from 350 to 600μm to 900μm led to a significant increase in the depth of MN penetration achieved. Moreover, alteration of MN interspacing had no effect upon depth of penetration achieved, at a constant MN height and force of application. With respect to MN dissolution, an approximate 34% reduction in MN height occurred in the first 15min, with only 17% of the MN height remaining after a 3-hour period. Across both skin models, there was a significantly greater cumulative amount of theophylline delivered after 24h from an MN array of 900μm height (292.23±16.77μg), in comparison to an MN array of 350μm height (242.62±14.81μg) (p<0.001). Employing full thickness skin significantly reduced drug permeation in both cases. Importantly, this study has highlighted the effect that MN geometry and application force have upon the depth of penetration into skin. While it has been shown that MN height has an important role in the extent of drug delivered across neonatal porcine skin from a soluble MN array, further studies to evaluate the full significance of MN geometry on MN mediated drug delivery are now underway. The successful use of OCT in this study could prove to be a key development for polymeric MN research, accelerating their commercial exploitation.

Authors+Show Affiliations

School of Pharmacy, Medical Biology Centre, Queen's University Belfast, 97 Lisburn Road, Belfast BT9 7BL, UK. r.donnelly@qub.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20727929

Citation

Donnelly, Ryan F., et al. "Optical Coherence Tomography Is a Valuable Tool in the Study of the Effects of Microneedle Geometry On Skin Penetration Characteristics and In-skin Dissolution." Journal of Controlled Release : Official Journal of the Controlled Release Society, vol. 147, no. 3, 2010, pp. 333-41.
Donnelly RF, Garland MJ, Morrow DI, et al. Optical coherence tomography is a valuable tool in the study of the effects of microneedle geometry on skin penetration characteristics and in-skin dissolution. J Control Release. 2010;147(3):333-41.
Donnelly, R. F., Garland, M. J., Morrow, D. I., Migalska, K., Singh, T. R., Majithiya, R., & Woolfson, A. D. (2010). Optical coherence tomography is a valuable tool in the study of the effects of microneedle geometry on skin penetration characteristics and in-skin dissolution. Journal of Controlled Release : Official Journal of the Controlled Release Society, 147(3), pp. 333-41. doi:10.1016/j.jconrel.2010.08.008.
Donnelly RF, et al. Optical Coherence Tomography Is a Valuable Tool in the Study of the Effects of Microneedle Geometry On Skin Penetration Characteristics and In-skin Dissolution. J Control Release. 2010 Nov 1;147(3):333-41. PubMed PMID: 20727929.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optical coherence tomography is a valuable tool in the study of the effects of microneedle geometry on skin penetration characteristics and in-skin dissolution. AU - Donnelly,Ryan F, AU - Garland,Martin J, AU - Morrow,Desmond I J, AU - Migalska,Katarzyna, AU - Singh,Thakur Raghu Raj, AU - Majithiya,Rita, AU - Woolfson,A David, Y1 - 2010/08/18/ PY - 2010/04/30/received PY - 2010/07/21/revised PY - 2010/08/09/accepted PY - 2010/8/24/entrez PY - 2010/8/24/pubmed PY - 2011/2/26/medline SP - 333 EP - 41 JF - Journal of controlled release : official journal of the Controlled Release Society JO - J Control Release VL - 147 IS - 3 N2 - In this study, we used optical coherence tomography (OCT) to extensively investigate, for the first time, the effect that microneedle (MN) geometry (MN height, and MN interspacing) and force of application have upon penetration characteristics of soluble poly(methylvinylether-co-maleic anhydride, PMVE/MA) MN arrays into neonatal porcine skin in vitro. The results from OCT investigations were then used to design optimal and suboptimal MN-based drug delivery systems and evaluate their drug delivery profiles cross full thickness and dermatomed neonatal porcine skin in vitro. It was found that increasing the force used for MN application resulted in a significant increase in the depth of penetration achieved within neonatal porcine skin. For example, MN of 600μm height penetrated to a depth of 330μm when inserted at a force of 4.4N/array, while the penetration increased significantly to a depth of 520μm, when the force of application was increased to 16.4N/array. At an application force of 11.0N/array it was found that, in each case, increasing MN height from 350 to 600μm to 900μm led to a significant increase in the depth of MN penetration achieved. Moreover, alteration of MN interspacing had no effect upon depth of penetration achieved, at a constant MN height and force of application. With respect to MN dissolution, an approximate 34% reduction in MN height occurred in the first 15min, with only 17% of the MN height remaining after a 3-hour period. Across both skin models, there was a significantly greater cumulative amount of theophylline delivered after 24h from an MN array of 900μm height (292.23±16.77μg), in comparison to an MN array of 350μm height (242.62±14.81μg) (p<0.001). Employing full thickness skin significantly reduced drug permeation in both cases. Importantly, this study has highlighted the effect that MN geometry and application force have upon the depth of penetration into skin. While it has been shown that MN height has an important role in the extent of drug delivered across neonatal porcine skin from a soluble MN array, further studies to evaluate the full significance of MN geometry on MN mediated drug delivery are now underway. The successful use of OCT in this study could prove to be a key development for polymeric MN research, accelerating their commercial exploitation. SN - 1873-4995 UR - https://www.unboundmedicine.com/medline/citation/20727929/Optical_coherence_tomography_is_a_valuable_tool_in_the_study_of_the_effects_of_microneedle_geometry_on_skin_penetration_characteristics_and_in_skin_dissolution_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168-3659(10)00663-2 DB - PRIME DP - Unbound Medicine ER -