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Optimisation of a simple and reliable method based on headspace solid-phase microextraction for the determination of volatile phenols in beer.
J Chromatogr A. 2010 Sep 24; 1217(39):6013-21.JC

Abstract

A simple, accurate and sensitive method based on headspace solid-phase microextraction (HS-SPME) coupled to gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed for the analysis of 4-ethylguaiacol, 4-ethylphenol, 4-vinylguaiacol and 4-vinylphenol in beer. The effect of the presence of CO2 in the sample on the extraction of analytes was examined. The influence on extraction efficiency of different fibre coatings, of salt addition and stirring was also evaluated. Divinylbenzene/carboxen/polydimethylsiloxane was selected as extraction fibre and was used to evaluate the influence of exposure time, extraction temperature and sample volume/total volume ratio (Vs/Vt) by means of a central composite design (CCD). The optimal conditions identified were 80 degrees C for extraction temperature, 55 min for extraction time and 6 mL of beer (Vs/Vt 0.30). Under optimal conditions, the proposed method showed satisfactory linearity (correlation coefficients between 0.993 and 0.999), precision (between 6.3% and 9.7%) and detection limits (lower than those previously reported for volatile phenols in beers). The method was applied successfully to the analysis of beer samples. To our knowledge, this is the first time that a HS-SPME based method has been developed to determine simultaneously these four volatile phenols in beers.

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Authors+Show Affiliations

Pizarro C
Department of Chemistry, University of La Rioja, C/Madre de Dios 51, 26006 Logroño, La Rioja, Spain. consuelo.pizarro@unirioja.es
Pérez-del-Notario N
No affiliation info available
González-Sáiz JM
No affiliation info available

MeSH

BeerDimethylpolysiloxanesGas Chromatography-Mass SpectrometryLeast-Squares AnalysisPhenolsPolyvinylsReproducibility of ResultsSensitivity and SpecificitySodium ChlorideSolid Phase MicroextractionTandem Mass SpectrometryTemperatureVolatile Organic Compounds

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20728896

Citation

Pizarro, C, et al. "Optimisation of a Simple and Reliable Method Based On Headspace Solid-phase Microextraction for the Determination of Volatile Phenols in Beer." Journal of Chromatography. A, vol. 1217, no. 39, 2010, pp. 6013-21.
Pizarro C, Pérez-del-Notario N, González-Sáiz JM. Optimisation of a simple and reliable method based on headspace solid-phase microextraction for the determination of volatile phenols in beer. J Chromatogr A. 2010;1217(39):6013-21.
Pizarro, C., Pérez-del-Notario, N., & González-Sáiz, J. M. (2010). Optimisation of a simple and reliable method based on headspace solid-phase microextraction for the determination of volatile phenols in beer. Journal of Chromatography. A, 1217(39), 6013-21. https://doi.org/10.1016/j.chroma.2010.07.021
Pizarro C, Pérez-del-Notario N, González-Sáiz JM. Optimisation of a Simple and Reliable Method Based On Headspace Solid-phase Microextraction for the Determination of Volatile Phenols in Beer. J Chromatogr A. 2010 Sep 24;1217(39):6013-21. PubMed PMID: 20728896.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Optimisation of a simple and reliable method based on headspace solid-phase microextraction for the determination of volatile phenols in beer. AU - Pizarro,C, AU - Pérez-del-Notario,N, AU - González-Sáiz,J M, Y1 - 2010/07/16/ PY - 2010/05/04/received PY - 2010/07/06/revised PY - 2010/07/10/accepted PY - 2010/8/24/entrez PY - 2010/8/24/pubmed PY - 2010/12/14/medline SP - 6013 EP - 21 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1217 IS - 39 N2 - A simple, accurate and sensitive method based on headspace solid-phase microextraction (HS-SPME) coupled to gas chromatography-tandem mass spectrometry (GC-MS/MS) was developed for the analysis of 4-ethylguaiacol, 4-ethylphenol, 4-vinylguaiacol and 4-vinylphenol in beer. The effect of the presence of CO2 in the sample on the extraction of analytes was examined. The influence on extraction efficiency of different fibre coatings, of salt addition and stirring was also evaluated. Divinylbenzene/carboxen/polydimethylsiloxane was selected as extraction fibre and was used to evaluate the influence of exposure time, extraction temperature and sample volume/total volume ratio (Vs/Vt) by means of a central composite design (CCD). The optimal conditions identified were 80 degrees C for extraction temperature, 55 min for extraction time and 6 mL of beer (Vs/Vt 0.30). Under optimal conditions, the proposed method showed satisfactory linearity (correlation coefficients between 0.993 and 0.999), precision (between 6.3% and 9.7%) and detection limits (lower than those previously reported for volatile phenols in beers). The method was applied successfully to the analysis of beer samples. To our knowledge, this is the first time that a HS-SPME based method has been developed to determine simultaneously these four volatile phenols in beers. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/20728896/Optimisation_of_a_simple_and_reliable_method_based_on_headspace_solid_phase_microextraction_for_the_determination_of_volatile_phenols_in_beer_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(10)00925-8 DB - PRIME DP - Unbound Medicine ER -
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