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Regional topographical differences of canine microglial immunophenotype and function in the healthy spinal cord.
J Neuroimmunol. 2010 Oct 08; 227(1-2):144-52.JN

Abstract

Differences in the regulation of surface molecule expression and functional activity of microglia, the resident immune effector elements of the central nervous system (CNS), might give important insights into understanding the predilection sites of some diseases within the CNS. Therefore, canine microglial cells in relation to different topographical regions within the healthy CNS were evaluated ex vivo from the brain, cervical, and thoracolumbar spinal cord using density gradient centrifugation and flow cytometry in a homogenous dog population. Immunophenotypical characterization showed physiological regional differences for B7-1, CD14, CD44, CD1c, CD18, CD11b, and CD11c. Both, phagocytosis and ROS generation revealed differences between the brain, cervical, and thoracolumbar spinal cord. Our results emphasize that microglia displays physiological topographical regional differences within the CNS. The dog seems to be an ideal model to further investigate the role of microglia in focal pathological conditions of the spinal cord.

Authors+Show Affiliations

Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover, Bünteweg 9, D-30559 Hannover, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20728950

Citation

Ensinger, Eva-Maria, et al. "Regional Topographical Differences of Canine Microglial Immunophenotype and Function in the Healthy Spinal Cord." Journal of Neuroimmunology, vol. 227, no. 1-2, 2010, pp. 144-52.
Ensinger EM, Boekhoff TM, Carlson R, et al. Regional topographical differences of canine microglial immunophenotype and function in the healthy spinal cord. J Neuroimmunol. 2010;227(1-2):144-52.
Ensinger, E. M., Boekhoff, T. M., Carlson, R., Beineke, A., Rohn, K., Tipold, A., & Stein, V. M. (2010). Regional topographical differences of canine microglial immunophenotype and function in the healthy spinal cord. Journal of Neuroimmunology, 227(1-2), 144-52. https://doi.org/10.1016/j.jneuroim.2010.07.017
Ensinger EM, et al. Regional Topographical Differences of Canine Microglial Immunophenotype and Function in the Healthy Spinal Cord. J Neuroimmunol. 2010 Oct 8;227(1-2):144-52. PubMed PMID: 20728950.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regional topographical differences of canine microglial immunophenotype and function in the healthy spinal cord. AU - Ensinger,Eva-Maria, AU - Boekhoff,Theda M A, AU - Carlson,Regina, AU - Beineke,Andreas, AU - Rohn,Karl, AU - Tipold,Andrea, AU - Stein,Veronika M, Y1 - 2010/08/21/ PY - 2010/04/22/received PY - 2010/07/07/revised PY - 2010/07/20/accepted PY - 2010/8/24/entrez PY - 2010/8/24/pubmed PY - 2010/10/20/medline SP - 144 EP - 52 JF - Journal of neuroimmunology JO - J. Neuroimmunol. VL - 227 IS - 1-2 N2 - Differences in the regulation of surface molecule expression and functional activity of microglia, the resident immune effector elements of the central nervous system (CNS), might give important insights into understanding the predilection sites of some diseases within the CNS. Therefore, canine microglial cells in relation to different topographical regions within the healthy CNS were evaluated ex vivo from the brain, cervical, and thoracolumbar spinal cord using density gradient centrifugation and flow cytometry in a homogenous dog population. Immunophenotypical characterization showed physiological regional differences for B7-1, CD14, CD44, CD1c, CD18, CD11b, and CD11c. Both, phagocytosis and ROS generation revealed differences between the brain, cervical, and thoracolumbar spinal cord. Our results emphasize that microglia displays physiological topographical regional differences within the CNS. The dog seems to be an ideal model to further investigate the role of microglia in focal pathological conditions of the spinal cord. SN - 1872-8421 UR - https://www.unboundmedicine.com/medline/citation/20728950/Regional_topographical_differences_of_canine_microglial_immunophenotype_and_function_in_the_healthy_spinal_cord_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-5728(10)00330-9 DB - PRIME DP - Unbound Medicine ER -