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Urate-responsive MarR homologs from Burkholderia.
Mol Biosyst. 2010 Nov; 6(11):2133-42.MB

Abstract

The genus Burkholderia includes a large number of species, some of which are serious human pathogens. A genomic locus is conserved that consists of a gene encoding a member of the multiple antibiotic resistance regulator (MarR) family of transcriptional regulators and a divergently oriented gene encoding a major facilitator transport protein (MFTP), a predicted membrane efflux pump. Homology modeling shows that the MarR homolog conserves the location of four conserved amino acid residues previously shown to bind the ligand urate in the Deinococcus radiodurans-encoded MarR homolog HucR. Analysis of the B. thailandensis-encoded homolog shows that its specific DNA binding to two adjacent sites in the intergenic region between the genes encoding the transcription factor and the MFTP is attenuated by urate and to a lesser extent by xanthine and hypoxanthine, but not by adenine or the product of urate degradation, allantoin. These data suggest the existence of a four amino acid urate-binding signature in a subset of MarR homologs, and that homologs bearing this signature will respond to the ligand urate by attenuated DNA binding. The location of binding sites predicts regulation of the MFTP and prompts a proposal to name the cognate transcription factor MftR (major facilitator transport regulator).

Authors+Show Affiliations

Department of Biological Sciences, Louisiana State University, Baton Rouge, LA 70803, USA. agrove@lsu.edu

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

20730247

Citation

Grove, Anne. "Urate-responsive MarR Homologs From Burkholderia." Molecular BioSystems, vol. 6, no. 11, 2010, pp. 2133-42.
Grove A. Urate-responsive MarR homologs from Burkholderia. Mol Biosyst. 2010;6(11):2133-42.
Grove, A. (2010). Urate-responsive MarR homologs from Burkholderia. Molecular BioSystems, 6(11), 2133-42. https://doi.org/10.1039/c0mb00086h
Grove A. Urate-responsive MarR Homologs From Burkholderia. Mol Biosyst. 2010;6(11):2133-42. PubMed PMID: 20730247.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Urate-responsive MarR homologs from Burkholderia. A1 - Grove,Anne, Y1 - 2010/08/23/ PY - 2010/8/24/entrez PY - 2010/8/24/pubmed PY - 2011/1/25/medline SP - 2133 EP - 42 JF - Molecular bioSystems JO - Mol Biosyst VL - 6 IS - 11 N2 - The genus Burkholderia includes a large number of species, some of which are serious human pathogens. A genomic locus is conserved that consists of a gene encoding a member of the multiple antibiotic resistance regulator (MarR) family of transcriptional regulators and a divergently oriented gene encoding a major facilitator transport protein (MFTP), a predicted membrane efflux pump. Homology modeling shows that the MarR homolog conserves the location of four conserved amino acid residues previously shown to bind the ligand urate in the Deinococcus radiodurans-encoded MarR homolog HucR. Analysis of the B. thailandensis-encoded homolog shows that its specific DNA binding to two adjacent sites in the intergenic region between the genes encoding the transcription factor and the MFTP is attenuated by urate and to a lesser extent by xanthine and hypoxanthine, but not by adenine or the product of urate degradation, allantoin. These data suggest the existence of a four amino acid urate-binding signature in a subset of MarR homologs, and that homologs bearing this signature will respond to the ligand urate by attenuated DNA binding. The location of binding sites predicts regulation of the MFTP and prompts a proposal to name the cognate transcription factor MftR (major facilitator transport regulator). SN - 1742-2051 UR - https://www.unboundmedicine.com/medline/citation/20730247/Urate_responsive_MarR_homologs_from_Burkholderia_ L2 - https://doi.org/10.1039/c0mb00086h DB - PRIME DP - Unbound Medicine ER -