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Antioxidant, antihyperuricemic and xanthine oxidase inhibitory activities of Hyoscyamus reticulatus.
Pharm Biol. 2010 Dec; 48(12):1376-83.PB

Abstract

CONTEXT

Xanthine oxidase (XO) is a key enzyme in the pathophysiological homeostasis of hyperuricemia. It catalyzes the oxidation of hypoxanthine to xanthine and then to uric acid, the reaction involves the formation of free radical intermediates and superoxide byproducts.

OBJECTIVES

This study was undertaken to investigate the antioxidant, antihyperuricemic, and xanthine oxidase inhibitory potentials of Hyoscyamus reticulatus L. (Solanaceae) extract.

MATERIALS AND METHODS

The antioxidant potency was measured using the ABTS•+ scavenging capacity system, which includes Trolox as a standard. The xanthine oxidase inhibitory activity of the extract was quantitated in vitro by measuring the decline in the catalytic rate of xanthine oxidase following incubations with the plant extracts and using xanthine as a substrate. The hypouricemic potential of the extract was evaluated using an in vivo model for hyperuricemia. We tested three different doses of the extract and allopurinol was used as standard antihyperuricemic positive control.

RESULTS

H. reticulatus aqueous extract exhibited significant antioxidant scavenging properties (533.26 μmol TE/g dry extract weight) and inhibitory effect on xanthine oxidase activity (IC₅₀ 12.8 μg/mL). Furthermore, oral administration of the aqueous extract significantly reduced serum urate levels in oxonate-induced hyperuricemic mice in a dose-dependent manner.

DISCUSSION AND CONCLUSION

Our results suggest that the aqueous extract of H. reticulatus aerial parts might have great potential as an antioxidant and a hypouricemic agent. Our lab is currently identifying the active compounds in the extract to which the biological activities could be attributed.

Authors+Show Affiliations

Faculty of Pharmacy, University of Jordan, Amman, Jordan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20738177

Citation

Mohammad, Mohammad K., et al. "Antioxidant, Antihyperuricemic and Xanthine Oxidase Inhibitory Activities of Hyoscyamus Reticulatus." Pharmaceutical Biology, vol. 48, no. 12, 2010, pp. 1376-83.
Mohammad MK, Almasri IM, Tawaha K, et al. Antioxidant, antihyperuricemic and xanthine oxidase inhibitory activities of Hyoscyamus reticulatus. Pharm Biol. 2010;48(12):1376-83.
Mohammad, M. K., Almasri, I. M., Tawaha, K., Issa, A., Al-Nadaf, A., Hudaib, M., Alkhatib, H. S., Abu-Gharbieh, E., & Bustanji, Y. (2010). Antioxidant, antihyperuricemic and xanthine oxidase inhibitory activities of Hyoscyamus reticulatus. Pharmaceutical Biology, 48(12), 1376-83. https://doi.org/10.3109/13880209.2010.483521
Mohammad MK, et al. Antioxidant, Antihyperuricemic and Xanthine Oxidase Inhibitory Activities of Hyoscyamus Reticulatus. Pharm Biol. 2010;48(12):1376-83. PubMed PMID: 20738177.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant, antihyperuricemic and xanthine oxidase inhibitory activities of Hyoscyamus reticulatus. AU - Mohammad,Mohammad K, AU - Almasri,Ihab M, AU - Tawaha,Khaled, AU - Issa,Ala, AU - Al-Nadaf,Afaf, AU - Hudaib,Mohammad, AU - Alkhatib,Hatim S, AU - Abu-Gharbieh,Eman, AU - Bustanji,Yasser, Y1 - 2010/08/25/ PY - 2010/8/27/entrez PY - 2010/8/27/pubmed PY - 2011/3/5/medline SP - 1376 EP - 83 JF - Pharmaceutical biology JO - Pharm Biol VL - 48 IS - 12 N2 - CONTEXT: Xanthine oxidase (XO) is a key enzyme in the pathophysiological homeostasis of hyperuricemia. It catalyzes the oxidation of hypoxanthine to xanthine and then to uric acid, the reaction involves the formation of free radical intermediates and superoxide byproducts. OBJECTIVES: This study was undertaken to investigate the antioxidant, antihyperuricemic, and xanthine oxidase inhibitory potentials of Hyoscyamus reticulatus L. (Solanaceae) extract. MATERIALS AND METHODS: The antioxidant potency was measured using the ABTS•+ scavenging capacity system, which includes Trolox as a standard. The xanthine oxidase inhibitory activity of the extract was quantitated in vitro by measuring the decline in the catalytic rate of xanthine oxidase following incubations with the plant extracts and using xanthine as a substrate. The hypouricemic potential of the extract was evaluated using an in vivo model for hyperuricemia. We tested three different doses of the extract and allopurinol was used as standard antihyperuricemic positive control. RESULTS: H. reticulatus aqueous extract exhibited significant antioxidant scavenging properties (533.26 μmol TE/g dry extract weight) and inhibitory effect on xanthine oxidase activity (IC₅₀ 12.8 μg/mL). Furthermore, oral administration of the aqueous extract significantly reduced serum urate levels in oxonate-induced hyperuricemic mice in a dose-dependent manner. DISCUSSION AND CONCLUSION: Our results suggest that the aqueous extract of H. reticulatus aerial parts might have great potential as an antioxidant and a hypouricemic agent. Our lab is currently identifying the active compounds in the extract to which the biological activities could be attributed. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/20738177/Antioxidant_antihyperuricemic_and_xanthine_oxidase_inhibitory_activities_of_Hyoscyamus_reticulatus_ L2 - http://www.tandfonline.com/doi/full/10.3109/13880209.2010.483521 DB - PRIME DP - Unbound Medicine ER -