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Improved detection of Pneumocystis carinii by an immunofluorescence technique using monoclonal antibodies.
Eur J Clin Microbiol Infect Dis. 1990 Dec; 9(12):880-5.EJ

Abstract

To assess whether a recently developed indirect immunofluorescent stain using monoclonal antibodies was more sensitive in detecting Pneumocystis carinii than the combination of Giemsa and methenamine silver nitrate stains which has routinely been used in the laboratory, 88 lavage fluid specimens and 34 induced sputum specimens were examined. All specimens were stained by five techniques: immunofluorescence using a combination of three monoclonal antibodies (from the National Institutes of Health, USA), immunofluorescence using a single monoclonal antibody (from Dakopatts), Giemsa, methenamine silver nitrate and toluidine blue O. Immunofluorescence using the monoclonal antibodies from the NIH was significantly more sensitive than any other single staining method and than the combination of Giemsa and methenamine silver nitrate staining. The study also showed that the cytospin centrifuge was very suitable for the preparation of slides with lavage fluid and processed induced sputum. Finally, the sensitivity of examination of induced sputum to detect Pneumocystis carinii was found to be 50% when compared with bronchoalveolar lavage fluid. However, this sensitivity may increase through practice.

Authors+Show Affiliations

Department of Infectious Diseases, Hvidovre Hospital, University of Copenhagen, Denmark.No affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

2073898

Citation

Orholm, M, et al. "Improved Detection of Pneumocystis Carinii By an Immunofluorescence Technique Using Monoclonal Antibodies." European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, vol. 9, no. 12, 1990, pp. 880-5.
Orholm M, Holten-Andersen W, Lundgren JD. Improved detection of Pneumocystis carinii by an immunofluorescence technique using monoclonal antibodies. Eur J Clin Microbiol Infect Dis. 1990;9(12):880-5.
Orholm, M., Holten-Andersen, W., & Lundgren, J. D. (1990). Improved detection of Pneumocystis carinii by an immunofluorescence technique using monoclonal antibodies. European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology, 9(12), 880-5.
Orholm M, Holten-Andersen W, Lundgren JD. Improved Detection of Pneumocystis Carinii By an Immunofluorescence Technique Using Monoclonal Antibodies. Eur J Clin Microbiol Infect Dis. 1990;9(12):880-5. PubMed PMID: 2073898.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improved detection of Pneumocystis carinii by an immunofluorescence technique using monoclonal antibodies. AU - Orholm,M, AU - Holten-Andersen,W, AU - Lundgren,J D, PY - 1990/12/1/pubmed PY - 1990/12/1/medline PY - 1990/12/1/entrez SP - 880 EP - 5 JF - European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology JO - Eur J Clin Microbiol Infect Dis VL - 9 IS - 12 N2 - To assess whether a recently developed indirect immunofluorescent stain using monoclonal antibodies was more sensitive in detecting Pneumocystis carinii than the combination of Giemsa and methenamine silver nitrate stains which has routinely been used in the laboratory, 88 lavage fluid specimens and 34 induced sputum specimens were examined. All specimens were stained by five techniques: immunofluorescence using a combination of three monoclonal antibodies (from the National Institutes of Health, USA), immunofluorescence using a single monoclonal antibody (from Dakopatts), Giemsa, methenamine silver nitrate and toluidine blue O. Immunofluorescence using the monoclonal antibodies from the NIH was significantly more sensitive than any other single staining method and than the combination of Giemsa and methenamine silver nitrate staining. The study also showed that the cytospin centrifuge was very suitable for the preparation of slides with lavage fluid and processed induced sputum. Finally, the sensitivity of examination of induced sputum to detect Pneumocystis carinii was found to be 50% when compared with bronchoalveolar lavage fluid. However, this sensitivity may increase through practice. SN - 0934-9723 UR - https://www.unboundmedicine.com/medline/citation/2073898/Improved_detection_of_Pneumocystis_carinii_by_an_immunofluorescence_technique_using_monoclonal_antibodies_ DB - PRIME DP - Unbound Medicine ER -