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Intrarenal suppression of angiotensin II type 1 receptor binding molecule in angiotensin II-infused mice.
Am J Physiol Renal Physiol. 2010 Nov; 299(5):F991-F1003.AJ

Abstract

ATRAP [ANG II type 1 receptor (AT1R)-associated protein] is a molecule which directly interacts with AT1R and inhibits AT1R signaling. The aim of this study was to examine the effects of continuous ANG II infusion on the intrarenal expression and distribution of ATRAP and to determine the role of AT1R signaling in mediating these effects. C57BL/6 male mice were subjected to vehicle or ANG II infusions at doses of 200, 1,000, or 2,500 ng·kg(-1)·min(-1) for 14 days. ANG II infusion caused significant suppression of ATRAP expression in the kidney but did not affect ATRAP expression in the testis or liver. Although only the highest ANG II dose (2,500 ng·kg(-1)·min(-1)) provoked renal pathological responses, such as an increase in the mRNA expression of angiotensinogen and the α-subunit of the epithelial sodium channel, ANG II-induced decreases in ATRAP were observed even at the lowest dose (200 ng·kg(-1)·min(-1)), particularly in the outer medulla of the kidney, based on immunohistochemical staining and Western blot analysis. The decrease in renal ATRAP expression by ANG II infusion was prevented by treatment with the AT1R-specific blocker olmesartan. In addition, the ANG II-mediated decrease in renal ATRAP expression through AT1R signaling occurred without an ANG II-induced decrease in plasma membrane AT1R expression in the kidney. On the other hand, a transgenic model increase in renal ATRAP expression beyond baseline was accompanied by a constitutive reduction of renal plasma membrane AT1R expression and by the promotion of renal AT1R internalization as well as the decreased induction of angiotensinogen gene expression in response to ANG II. These results suggest that the plasma membrane AT1R level in the kidney is modulated by intrarenal ATRAP expression under physiological and pathophysiological conditions in vivo.

Authors+Show Affiliations

Dept. of Medical Science and Cardiorenal Medicine, Yokohama City Univ. Graduate School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20739392

Citation

Wakui, Hiromichi, et al. "Intrarenal Suppression of Angiotensin II Type 1 Receptor Binding Molecule in Angiotensin II-infused Mice." American Journal of Physiology. Renal Physiology, vol. 299, no. 5, 2010, pp. F991-F1003.
Wakui H, Tamura K, Matsuda M, et al. Intrarenal suppression of angiotensin II type 1 receptor binding molecule in angiotensin II-infused mice. Am J Physiol Renal Physiol. 2010;299(5):F991-F1003.
Wakui, H., Tamura, K., Matsuda, M., Bai, Y., Dejima, T., Shigenaga, A., Masuda, S., Azuma, K., Maeda, A., Hirose, T., Ishigami, T., Toya, Y., Yabana, M., Minamisawa, S., & Umemura, S. (2010). Intrarenal suppression of angiotensin II type 1 receptor binding molecule in angiotensin II-infused mice. American Journal of Physiology. Renal Physiology, 299(5), F991-F1003. https://doi.org/10.1152/ajprenal.00738.2009
Wakui H, et al. Intrarenal Suppression of Angiotensin II Type 1 Receptor Binding Molecule in Angiotensin II-infused Mice. Am J Physiol Renal Physiol. 2010;299(5):F991-F1003. PubMed PMID: 20739392.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intrarenal suppression of angiotensin II type 1 receptor binding molecule in angiotensin II-infused mice. AU - Wakui,Hiromichi, AU - Tamura,Kouichi, AU - Matsuda,Miyuki, AU - Bai,Yunzhe, AU - Dejima,Toru, AU - Shigenaga,Atsu-Ichiro, AU - Masuda,Shin-Ichiro, AU - Azuma,Koichi, AU - Maeda,Akinobu, AU - Hirose,Tomonori, AU - Ishigami,Tomoaki, AU - Toya,Yoshiyuki, AU - Yabana,Machiko, AU - Minamisawa,Susumu, AU - Umemura,Satoshi, Y1 - 2010/08/25/ PY - 2010/8/27/entrez PY - 2010/8/27/pubmed PY - 2010/12/14/medline SP - F991 EP - F1003 JF - American journal of physiology. Renal physiology JO - Am. J. Physiol. Renal Physiol. VL - 299 IS - 5 N2 - ATRAP [ANG II type 1 receptor (AT1R)-associated protein] is a molecule which directly interacts with AT1R and inhibits AT1R signaling. The aim of this study was to examine the effects of continuous ANG II infusion on the intrarenal expression and distribution of ATRAP and to determine the role of AT1R signaling in mediating these effects. C57BL/6 male mice were subjected to vehicle or ANG II infusions at doses of 200, 1,000, or 2,500 ng·kg(-1)·min(-1) for 14 days. ANG II infusion caused significant suppression of ATRAP expression in the kidney but did not affect ATRAP expression in the testis or liver. Although only the highest ANG II dose (2,500 ng·kg(-1)·min(-1)) provoked renal pathological responses, such as an increase in the mRNA expression of angiotensinogen and the α-subunit of the epithelial sodium channel, ANG II-induced decreases in ATRAP were observed even at the lowest dose (200 ng·kg(-1)·min(-1)), particularly in the outer medulla of the kidney, based on immunohistochemical staining and Western blot analysis. The decrease in renal ATRAP expression by ANG II infusion was prevented by treatment with the AT1R-specific blocker olmesartan. In addition, the ANG II-mediated decrease in renal ATRAP expression through AT1R signaling occurred without an ANG II-induced decrease in plasma membrane AT1R expression in the kidney. On the other hand, a transgenic model increase in renal ATRAP expression beyond baseline was accompanied by a constitutive reduction of renal plasma membrane AT1R expression and by the promotion of renal AT1R internalization as well as the decreased induction of angiotensinogen gene expression in response to ANG II. These results suggest that the plasma membrane AT1R level in the kidney is modulated by intrarenal ATRAP expression under physiological and pathophysiological conditions in vivo. SN - 1522-1466 UR - https://www.unboundmedicine.com/medline/citation/20739392/Intrarenal_suppression_of_angiotensin_II_type_1_receptor_binding_molecule_in_angiotensin_II_infused_mice_ L2 - http://www.physiology.org/doi/full/10.1152/ajprenal.00738.2009?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -