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Amino acid sequence and disulfide bridges of affinity purified Kunitz-type chymotrypsin inhibitor from winged bean seed (Psophocarpus tetragonolobus (L.) DC).
Biochem Int. 1990 Nov; 22(3):543-51.BI

Abstract

The primary sequence of the affinity purified chymotrypsin inhibitor, WBCI, isolated from the albumin fraction of Psophocarpus tetragonolobus (L.) DC cv. UPS-122 seed was determined. The inhibitor consisted of a single polypeptide chain of 183 amino acids (Mr 20285) and the four half-cystine residues in the molecule formed two intramolecular disulfide bridges equivalent to those in other Kunitz-type seed inhibitors. The sequence of this chymotrypsin inhibitor was identical to that of chymotrypsin inhibitor-3 from cultivar UPS-31 and it showed about 50% sequence similarity to the winged bean acidic (WBTI-2, pI 5.1) and basic (WBTI-1, pI 8.9) trypsin inhibitors. Sequence similarities to other Kunitz-type seed inhibitors are discussed.

Authors+Show Affiliations

Kortt AA
CSIRO, Division of Biomolecular Engineering, Parkville Laboratory, Victoria, Australia.
Burns JE
No affiliation info available
Strike PM
No affiliation info available

MeSH

Amino Acid SequenceAmino AcidsChromatography, AffinityDisulfidesFabaceaeMolecular Sequence DataPlant ProteinsPlants, MedicinalSequence Homology, Nucleic AcidTrypsin Inhibitors

Pub Type(s)

Journal Article

Language

eng

PubMed ID

2076111

Citation

Kortt, A A., et al. "Amino Acid Sequence and Disulfide Bridges of Affinity Purified Kunitz-type Chymotrypsin Inhibitor From Winged Bean Seed (Psophocarpus Tetragonolobus (L.) DC)." Biochemistry International, vol. 22, no. 3, 1990, pp. 543-51.
Kortt AA, Burns JE, Strike PM. Amino acid sequence and disulfide bridges of affinity purified Kunitz-type chymotrypsin inhibitor from winged bean seed (Psophocarpus tetragonolobus (L.) DC). Biochem Int. 1990;22(3):543-51.
Kortt, A. A., Burns, J. E., & Strike, P. M. (1990). Amino acid sequence and disulfide bridges of affinity purified Kunitz-type chymotrypsin inhibitor from winged bean seed (Psophocarpus tetragonolobus (L.) DC). Biochemistry International, 22(3), 543-51.
Kortt AA, Burns JE, Strike PM. Amino Acid Sequence and Disulfide Bridges of Affinity Purified Kunitz-type Chymotrypsin Inhibitor From Winged Bean Seed (Psophocarpus Tetragonolobus (L.) DC). Biochem Int. 1990;22(3):543-51. PubMed PMID: 2076111.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amino acid sequence and disulfide bridges of affinity purified Kunitz-type chymotrypsin inhibitor from winged bean seed (Psophocarpus tetragonolobus (L.) DC). AU - Kortt,A A, AU - Burns,J E, AU - Strike,P M, PY - 1990/11/1/pubmed PY - 1990/11/1/medline PY - 1990/11/1/entrez SP - 543 EP - 51 JF - Biochemistry international JO - Biochem Int VL - 22 IS - 3 N2 - The primary sequence of the affinity purified chymotrypsin inhibitor, WBCI, isolated from the albumin fraction of Psophocarpus tetragonolobus (L.) DC cv. UPS-122 seed was determined. The inhibitor consisted of a single polypeptide chain of 183 amino acids (Mr 20285) and the four half-cystine residues in the molecule formed two intramolecular disulfide bridges equivalent to those in other Kunitz-type seed inhibitors. The sequence of this chymotrypsin inhibitor was identical to that of chymotrypsin inhibitor-3 from cultivar UPS-31 and it showed about 50% sequence similarity to the winged bean acidic (WBTI-2, pI 5.1) and basic (WBTI-1, pI 8.9) trypsin inhibitors. Sequence similarities to other Kunitz-type seed inhibitors are discussed. SN - 0158-5231 UR - https://www.unboundmedicine.com/medline/citation/2076111/Amino_acid_sequence_and_disulfide_bridges_of_affinity_purified_Kunitz_type_chymotrypsin_inhibitor_from_winged_bean_seed__Psophocarpus_tetragonolobus__L___DC__ DB - PRIME DP - Unbound Medicine ER -