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Accuracy of the Kato-Katz, adhesive tape and FLOTAC techniques for helminth diagnosis among children in Kyrgyzstan.
Acta Trop. 2010 Dec; 116(3):185-92.AT

Abstract

The purpose of this study was to assess the accuracy of three copro-microscopic techniques for helminth diagnosis: Kato-Katz, adhesive tape and FLOTAC. A total of 163 children from a peri-urban municipality near Bishkek, Kyrgyzstan, participated and submitted multiple stool samples and adhesive tapes. Ninety children supplied at least two stool samples and two adhesive tapes. Three stool samples and three adhesive tapes were available from 71 and 64 children, respectively. From each stool sample, a single Kato-Katz thick smear was prepared and examined quantitatively. Additionally, the first stool sample was subjected to the FLOTAC technique and helminth eggs were counted. Adhesive tapes were checked for the presence of Enterobius vermicularis eggs. Using pooled results as a diagnostic 'gold' standard, the prevalence of Ascaris lumbricoides, E. vermicularis, Hymenolepis nana and Dicrocoelium dendriticum were 54.4%, 13.3%, 11.1% and 11.1%, respectively. Infection intensities were low. When compared to triplicate Kato-Katz, a single FLOTAC was more sensitive for the diagnosis of A. lumbricoides (89.5% versus 39.5%) and D. dendriticum (88.9% versus 33.3%), but less sensitive for H. nana (66.7% versus 88.9%). For E. vermicularis, three adhesive tapes showed much higher sensitivity than a single FLOTAC (92.9% versus 14.3%). FLOTAC yielded significantly higher faecal egg counts than Kato-Katz for A. lumbricoides and D. dendriticum. Overall results suggest that, although FLOTAC represents a promising technique for helminth diagnosis in Kyrgyzstan, the repeated adhesive tape test remains so far the method of choice for diagnosing E. vermicularis.

Authors+Show Affiliations

Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, UK. ajeandron@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20800568

Citation

Jeandron, Aurelie, et al. "Accuracy of the Kato-Katz, Adhesive Tape and FLOTAC Techniques for Helminth Diagnosis Among Children in Kyrgyzstan." Acta Tropica, vol. 116, no. 3, 2010, pp. 185-92.
Jeandron A, Abdyldaieva G, Usubalieva J, et al. Accuracy of the Kato-Katz, adhesive tape and FLOTAC techniques for helminth diagnosis among children in Kyrgyzstan. Acta Trop. 2010;116(3):185-92.
Jeandron, A., Abdyldaieva, G., Usubalieva, J., Ensink, J. H., Cox, J., Matthys, B., Rinaldi, L., Cringoli, G., & Utzinger, J. (2010). Accuracy of the Kato-Katz, adhesive tape and FLOTAC techniques for helminth diagnosis among children in Kyrgyzstan. Acta Tropica, 116(3), 185-92. https://doi.org/10.1016/j.actatropica.2010.08.010
Jeandron A, et al. Accuracy of the Kato-Katz, Adhesive Tape and FLOTAC Techniques for Helminth Diagnosis Among Children in Kyrgyzstan. Acta Trop. 2010;116(3):185-92. PubMed PMID: 20800568.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Accuracy of the Kato-Katz, adhesive tape and FLOTAC techniques for helminth diagnosis among children in Kyrgyzstan. AU - Jeandron,Aurelie, AU - Abdyldaieva,Gulnara, AU - Usubalieva,Jumagul, AU - Ensink,Jeroen H J, AU - Cox,Jonathan, AU - Matthys,Barbara, AU - Rinaldi,Laura, AU - Cringoli,Giuseppe, AU - Utzinger,Jürg, Y1 - 2010/08/26/ PY - 2009/06/16/received PY - 2010/08/16/revised PY - 2010/08/17/accepted PY - 2010/8/31/entrez PY - 2010/8/31/pubmed PY - 2011/1/19/medline SP - 185 EP - 92 JF - Acta tropica JO - Acta Trop VL - 116 IS - 3 N2 - The purpose of this study was to assess the accuracy of three copro-microscopic techniques for helminth diagnosis: Kato-Katz, adhesive tape and FLOTAC. A total of 163 children from a peri-urban municipality near Bishkek, Kyrgyzstan, participated and submitted multiple stool samples and adhesive tapes. Ninety children supplied at least two stool samples and two adhesive tapes. Three stool samples and three adhesive tapes were available from 71 and 64 children, respectively. From each stool sample, a single Kato-Katz thick smear was prepared and examined quantitatively. Additionally, the first stool sample was subjected to the FLOTAC technique and helminth eggs were counted. Adhesive tapes were checked for the presence of Enterobius vermicularis eggs. Using pooled results as a diagnostic 'gold' standard, the prevalence of Ascaris lumbricoides, E. vermicularis, Hymenolepis nana and Dicrocoelium dendriticum were 54.4%, 13.3%, 11.1% and 11.1%, respectively. Infection intensities were low. When compared to triplicate Kato-Katz, a single FLOTAC was more sensitive for the diagnosis of A. lumbricoides (89.5% versus 39.5%) and D. dendriticum (88.9% versus 33.3%), but less sensitive for H. nana (66.7% versus 88.9%). For E. vermicularis, three adhesive tapes showed much higher sensitivity than a single FLOTAC (92.9% versus 14.3%). FLOTAC yielded significantly higher faecal egg counts than Kato-Katz for A. lumbricoides and D. dendriticum. Overall results suggest that, although FLOTAC represents a promising technique for helminth diagnosis in Kyrgyzstan, the repeated adhesive tape test remains so far the method of choice for diagnosing E. vermicularis. SN - 1873-6254 UR - https://www.unboundmedicine.com/medline/citation/20800568/Accuracy_of_the_Kato_Katz_adhesive_tape_and_FLOTAC_techniques_for_helminth_diagnosis_among_children_in_Kyrgyzstan_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0001-706X(10)00212-3 DB - PRIME DP - Unbound Medicine ER -