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Multiple induced mutagenesis for improvement of ethanol production by Kluyveromyces marxianus.
Biotechnol Lett. 2010 Dec; 32(12):1847-51.BL

Abstract

Kluyveromyces marxianus GX-15 was mutated multiple times by alternately treatment with UV irradiation and NTG for two cycles. Four mutant strains with improved ethanol yield were obtained. The maximum ethanol concentration, ethanol yield coefficient and theoretical ethanol yield of the best mutant strain, GX-UN120, was 69 g/l, 0.46 g/g and 91%, respectively, when fermenting 150 g glucose/l at 40°C. The corresponding values for GX-15 were 58 g/l, 0.39 g/g and 76%, respectively. GX-UN120 grew well in 11% (v/v) of ethanol, while GX-15 could not grow when ethanol was greater than 8% (v/v).

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Authors+Show Affiliations

Pang ZW
College of Life Science and Technology, Guangxi University, Nanning, Guangxi, 530005, China.
Liang JJ
No affiliation info available
Qin XJ
No affiliation info available
Wang JR
No affiliation info available
Feng JX
No affiliation info available
Huang RB
No affiliation info available

MeSH

EthanolFermentationGlucoseKluyveromycesMetabolic Networks and PathwaysMolecular Sequence DataMutagenesisMutagensNitrosoguanidinesSequence Analysis, DNAUltraviolet Rays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20803163

Citation

Pang, Zong-wen, et al. "Multiple Induced Mutagenesis for Improvement of Ethanol Production By Kluyveromyces Marxianus." Biotechnology Letters, vol. 32, no. 12, 2010, pp. 1847-51.
Pang ZW, Liang JJ, Qin XJ, et al. Multiple induced mutagenesis for improvement of ethanol production by Kluyveromyces marxianus. Biotechnol Lett. 2010;32(12):1847-51.
Pang, Z. W., Liang, J. J., Qin, X. J., Wang, J. R., Feng, J. X., & Huang, R. B. (2010). Multiple induced mutagenesis for improvement of ethanol production by Kluyveromyces marxianus. Biotechnology Letters, 32(12), 1847-51. https://doi.org/10.1007/s10529-010-0384-8
Pang ZW, et al. Multiple Induced Mutagenesis for Improvement of Ethanol Production By Kluyveromyces Marxianus. Biotechnol Lett. 2010;32(12):1847-51. PubMed PMID: 20803163.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiple induced mutagenesis for improvement of ethanol production by Kluyveromyces marxianus. AU - Pang,Zong-wen, AU - Liang,Jing-juan, AU - Qin,Xiao-juan, AU - Wang,Ji-rui, AU - Feng,Jia-xun, AU - Huang,Ri-bo, Y1 - 2010/08/28/ PY - 2010/07/04/received PY - 2010/08/18/accepted PY - 2010/8/31/entrez PY - 2010/8/31/pubmed PY - 2011/2/11/medline SP - 1847 EP - 51 JF - Biotechnology letters JO - Biotechnol Lett VL - 32 IS - 12 N2 - Kluyveromyces marxianus GX-15 was mutated multiple times by alternately treatment with UV irradiation and NTG for two cycles. Four mutant strains with improved ethanol yield were obtained. The maximum ethanol concentration, ethanol yield coefficient and theoretical ethanol yield of the best mutant strain, GX-UN120, was 69 g/l, 0.46 g/g and 91%, respectively, when fermenting 150 g glucose/l at 40°C. The corresponding values for GX-15 were 58 g/l, 0.39 g/g and 76%, respectively. GX-UN120 grew well in 11% (v/v) of ethanol, while GX-15 could not grow when ethanol was greater than 8% (v/v). SN - 1573-6776 UR - https://www.unboundmedicine.com/medline/citation/20803163/Multiple_induced_mutagenesis_for_improvement_of_ethanol_production_by_Kluyveromyces_marxianus_ L2 - https://doi.org/10.1007/s10529-010-0384-8 DB - PRIME DP - Unbound Medicine ER -
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