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Cloning and expression in E. coli of an organic solvent-tolerant and alkali-resistant glucose 1-dehydrogenase from Lysinibacillus sphaericus G10.
Bioresour Technol. 2011 Jan; 102(2):1528-36.BT

Abstract

The gene gdh encoding an organic solvent-tolerant and alkaline-resistant NAD(P)-dependent glucose 1-dehydrogenase (LsGDH) was cloned from Lysinibacillus sphaericus G10 and expressed in Escherichia coli. The recombinant LsGDH exhibited maximum activity at pH 9.5 and 50 °C. LsGDH displayed high stability at a wide pH ranging from 6.5 to 10.0 and was stable after incubation at 30 °C for 1 week in 25 mM sodium phosphate buffer (pH 6.5) in the absence or presence of NaCl. The activity of LsGDH was enhanced by Li+, Na+, K+, NH4+, Mg2+, and EDTA at pH 8.0. LsGDH exhibited high tolerance to 60% DMSO, 30% acetone, 30% methanol, 30% ethanol, 10% n-propanol, 30% isopropanol, 60% n-hexanol and 30% n-hexane. The relationship between stability and chain length of the alcohols fit a Gaussian distribution model (R2≥0.94), and demonstrated lowest enzyme stability in C4-alcohol. The results suggested that LsGDH was potentially useful for coenzyme regeneration in organic solvents or under alkaline conditions.

Authors+Show Affiliations

Institute of Microbiology, College of Life Science, Zhejiang University, Hangzhou 310058, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20805024

Citation

Ding, Hai-Tao, et al. "Cloning and Expression in E. Coli of an Organic Solvent-tolerant and Alkali-resistant Glucose 1-dehydrogenase From Lysinibacillus Sphaericus G10." Bioresource Technology, vol. 102, no. 2, 2011, pp. 1528-36.
Ding HT, Du YQ, Liu DF, et al. Cloning and expression in E. coli of an organic solvent-tolerant and alkali-resistant glucose 1-dehydrogenase from Lysinibacillus sphaericus G10. Bioresour Technol. 2011;102(2):1528-36.
Ding, H. T., Du, Y. Q., Liu, D. F., Li, Z. L., Chen, X. J., & Zhao, Y. H. (2011). Cloning and expression in E. coli of an organic solvent-tolerant and alkali-resistant glucose 1-dehydrogenase from Lysinibacillus sphaericus G10. Bioresource Technology, 102(2), 1528-36. https://doi.org/10.1016/j.biortech.2010.08.018
Ding HT, et al. Cloning and Expression in E. Coli of an Organic Solvent-tolerant and Alkali-resistant Glucose 1-dehydrogenase From Lysinibacillus Sphaericus G10. Bioresour Technol. 2011;102(2):1528-36. PubMed PMID: 20805024.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and expression in E. coli of an organic solvent-tolerant and alkali-resistant glucose 1-dehydrogenase from Lysinibacillus sphaericus G10. AU - Ding,Hai-Tao, AU - Du,Yi-Qing, AU - Liu,Dan-Feng, AU - Li,Ze-Li, AU - Chen,Xue-Jiao, AU - Zhao,Yu-Hua, Y1 - 2010/08/11/ PY - 2010/04/04/received PY - 2010/07/28/revised PY - 2010/08/04/accepted PY - 2010/9/1/entrez PY - 2010/9/2/pubmed PY - 2011/4/22/medline SP - 1528 EP - 36 JF - Bioresource technology JO - Bioresour. Technol. VL - 102 IS - 2 N2 - The gene gdh encoding an organic solvent-tolerant and alkaline-resistant NAD(P)-dependent glucose 1-dehydrogenase (LsGDH) was cloned from Lysinibacillus sphaericus G10 and expressed in Escherichia coli. The recombinant LsGDH exhibited maximum activity at pH 9.5 and 50 °C. LsGDH displayed high stability at a wide pH ranging from 6.5 to 10.0 and was stable after incubation at 30 °C for 1 week in 25 mM sodium phosphate buffer (pH 6.5) in the absence or presence of NaCl. The activity of LsGDH was enhanced by Li+, Na+, K+, NH4+, Mg2+, and EDTA at pH 8.0. LsGDH exhibited high tolerance to 60% DMSO, 30% acetone, 30% methanol, 30% ethanol, 10% n-propanol, 30% isopropanol, 60% n-hexanol and 30% n-hexane. The relationship between stability and chain length of the alcohols fit a Gaussian distribution model (R2≥0.94), and demonstrated lowest enzyme stability in C4-alcohol. The results suggested that LsGDH was potentially useful for coenzyme regeneration in organic solvents or under alkaline conditions. SN - 1873-2976 UR - https://www.unboundmedicine.com/medline/citation/20805024/Cloning_and_expression_in_E__coli_of_an_organic_solvent_tolerant_and_alkali_resistant_glucose_1_dehydrogenase_from_Lysinibacillus_sphaericus_G10_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0960-8524(10)01378-7 DB - PRIME DP - Unbound Medicine ER -