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Iron chelation by cranberry juice and its impact on Escherichia coli growth.
Biofactors. 2011 Mar-Apr; 37(2):121-30.B

Abstract

The various health benefits of Vaccinium macrocarpon (cranberry) are well documented and have been attributed mainly to its antioxidant capacity and anti-adhesive activity. Several different mechanisms have been proposed to explain the possible role of cranberries, cranberry juice, and cranberry extracts in inhibiting bacterial growth. These mechanisms of action (i.e., inhibition of the microbial growth) have not been thoroughly studied. Here, we took advantage of current advances in microarray technology and used GeneChip® Escherichia coli genome 2.0 arrays to gain insight into the molecular mechanisms involved in the impact of cranberry juice on the properties of E. coli growth. The inclusion of cranberry juice in bacterial growth media was found to significantly impact the doubling time of E. coli. The gene expression results revealed altered expression of genes associated with iron transport and essential metabolic enzymes as well as with adenosine triphosphate (ATP) synthesis and fumarate hydratase in these cultures. The altered expression of genes associated with iron transport was consistent with the strong iron chelating capability of proanthocyanidins, a major constituent of cranberry juice. The iron depletion effect was confirmed by adding exogenous iron to the growth media. This addition partially reversed the inhibitory effect on bacterial growth observed in the presence of cranberry juice/extracts.

Authors+Show Affiliations

Center for Bio/Molecular Science and Engineering, U.S. Naval Research Laboratory, Washington DC, USA. baochuan.lin@nrl.navy.milNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

20806417

Citation

Lin, Baochuan, et al. "Iron Chelation By Cranberry Juice and Its Impact On Escherichia Coli Growth." BioFactors (Oxford, England), vol. 37, no. 2, 2011, pp. 121-30.
Lin B, Johnson BJ, Rubin RA, et al. Iron chelation by cranberry juice and its impact on Escherichia coli growth. Biofactors. 2011;37(2):121-30.
Lin, B., Johnson, B. J., Rubin, R. A., Malanoski, A. P., & Ligler, F. S. (2011). Iron chelation by cranberry juice and its impact on Escherichia coli growth. BioFactors (Oxford, England), 37(2), 121-30. https://doi.org/10.1002/biof.110
Lin B, et al. Iron Chelation By Cranberry Juice and Its Impact On Escherichia Coli Growth. Biofactors. 2011 Mar-Apr;37(2):121-30. PubMed PMID: 20806417.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Iron chelation by cranberry juice and its impact on Escherichia coli growth. AU - Lin,Baochuan, AU - Johnson,Brandy J, AU - Rubin,Robert A, AU - Malanoski,Anthony P, AU - Ligler,Frances S, Y1 - 2010/08/30/ PY - 2010/01/11/received PY - 2010/06/10/accepted PY - 2010/9/1/entrez PY - 2010/9/2/pubmed PY - 2011/8/31/medline SP - 121 EP - 30 JF - BioFactors (Oxford, England) JO - Biofactors VL - 37 IS - 2 N2 - The various health benefits of Vaccinium macrocarpon (cranberry) are well documented and have been attributed mainly to its antioxidant capacity and anti-adhesive activity. Several different mechanisms have been proposed to explain the possible role of cranberries, cranberry juice, and cranberry extracts in inhibiting bacterial growth. These mechanisms of action (i.e., inhibition of the microbial growth) have not been thoroughly studied. Here, we took advantage of current advances in microarray technology and used GeneChip® Escherichia coli genome 2.0 arrays to gain insight into the molecular mechanisms involved in the impact of cranberry juice on the properties of E. coli growth. The inclusion of cranberry juice in bacterial growth media was found to significantly impact the doubling time of E. coli. The gene expression results revealed altered expression of genes associated with iron transport and essential metabolic enzymes as well as with adenosine triphosphate (ATP) synthesis and fumarate hydratase in these cultures. The altered expression of genes associated with iron transport was consistent with the strong iron chelating capability of proanthocyanidins, a major constituent of cranberry juice. The iron depletion effect was confirmed by adding exogenous iron to the growth media. This addition partially reversed the inhibitory effect on bacterial growth observed in the presence of cranberry juice/extracts. SN - 1872-8081 UR - https://www.unboundmedicine.com/medline/citation/20806417/Iron_chelation_by_cranberry_juice_and_its_impact_on_Escherichia_coli_growth_ L2 - https://doi.org/10.1002/biof.110 DB - PRIME DP - Unbound Medicine ER -