Tags

Type your tag names separated by a space and hit enter

Phytochemicals from Acacia confusa heartwood extracts reduce serum uric acid levels in oxonate-induced mice: their potential use as xanthine oxidase inhibitors.
J Agric Food Chem. 2010 Sep 22; 58(18):9936-41.JA

Abstract

In this study, the antihyperuricemic effect of Acacia confusa heartwood extracts and their phytochemicals on potassium oxonate (PO)-induced acute hyperuricemia was investigated for the first time. All treatments at the same dosage (100 mmol/kg) were administered to the abdominal cavity of PO-induced hyperuricemic mice, and serum uric acid level was measured at 3 h after administration. In experimental mice, serum uric acid level was significantly suppressed by the administration of A. confusa heartwood extracts and their major phytochemicals, (-)-2,3-cis-3,4-cis-3,3',4,4',7,8-hexahydroxyflavan, (-)-2,3-cis-3,4-cis-4'-methoxy-3,3',4,7,8-pentahydroxyflavan, melanoxetin, transilitin, and okanin, relative to the PO group. The direct inhibitory effect of these five compounds on xanthine oxidase (XOD) activity was examined using isothermal titration calorimetry (ITC). Among them, melanoxetin showed a more remarkable inhibitory effect on XOD activity than allopurinol, a clinical drug used for XOD inhibitor. To further understand the stereochemistry between XOD and melanoxetin (or allopurinol), structure-based molecular modeling was performed. Melanoxetin undergoes extended interactions in the hydrophobic region via the 3',4'-dihydroxyphenyl moiety, thus accounting for its higher binding affinity to XOD than allopurinol. These results indicate that A. confusa heartwood extracts and their major phytochemicals exhibit strong XOD inhibitory effects, which reduce serum uric acid levels while inhibiting uric acid generation in purine metabolism.

Authors+Show Affiliations

School of Forestry and Resource Conservation, National Taiwan University, Taipei 106, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20806936

Citation

Tung, Yu-Tang, et al. "Phytochemicals From Acacia Confusa Heartwood Extracts Reduce Serum Uric Acid Levels in Oxonate-induced Mice: Their Potential Use as Xanthine Oxidase Inhibitors." Journal of Agricultural and Food Chemistry, vol. 58, no. 18, 2010, pp. 9936-41.
Tung YT, Hsu CA, Chen CS, et al. Phytochemicals from Acacia confusa heartwood extracts reduce serum uric acid levels in oxonate-induced mice: their potential use as xanthine oxidase inhibitors. J Agric Food Chem. 2010;58(18):9936-41.
Tung, Y. T., Hsu, C. A., Chen, C. S., Yang, S. C., Huang, C. C., & Chang, S. T. (2010). Phytochemicals from Acacia confusa heartwood extracts reduce serum uric acid levels in oxonate-induced mice: their potential use as xanthine oxidase inhibitors. Journal of Agricultural and Food Chemistry, 58(18), 9936-41. https://doi.org/10.1021/jf102689k
Tung YT, et al. Phytochemicals From Acacia Confusa Heartwood Extracts Reduce Serum Uric Acid Levels in Oxonate-induced Mice: Their Potential Use as Xanthine Oxidase Inhibitors. J Agric Food Chem. 2010 Sep 22;58(18):9936-41. PubMed PMID: 20806936.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phytochemicals from Acacia confusa heartwood extracts reduce serum uric acid levels in oxonate-induced mice: their potential use as xanthine oxidase inhibitors. AU - Tung,Yu-Tang, AU - Hsu,Chih-An, AU - Chen,Chien-Shu, AU - Yang,Suh-Ching, AU - Huang,Chi-Chang, AU - Chang,Shang-Tzen, PY - 2010/9/3/entrez PY - 2010/9/3/pubmed PY - 2011/1/15/medline SP - 9936 EP - 41 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 58 IS - 18 N2 - In this study, the antihyperuricemic effect of Acacia confusa heartwood extracts and their phytochemicals on potassium oxonate (PO)-induced acute hyperuricemia was investigated for the first time. All treatments at the same dosage (100 mmol/kg) were administered to the abdominal cavity of PO-induced hyperuricemic mice, and serum uric acid level was measured at 3 h after administration. In experimental mice, serum uric acid level was significantly suppressed by the administration of A. confusa heartwood extracts and their major phytochemicals, (-)-2,3-cis-3,4-cis-3,3',4,4',7,8-hexahydroxyflavan, (-)-2,3-cis-3,4-cis-4'-methoxy-3,3',4,7,8-pentahydroxyflavan, melanoxetin, transilitin, and okanin, relative to the PO group. The direct inhibitory effect of these five compounds on xanthine oxidase (XOD) activity was examined using isothermal titration calorimetry (ITC). Among them, melanoxetin showed a more remarkable inhibitory effect on XOD activity than allopurinol, a clinical drug used for XOD inhibitor. To further understand the stereochemistry between XOD and melanoxetin (or allopurinol), structure-based molecular modeling was performed. Melanoxetin undergoes extended interactions in the hydrophobic region via the 3',4'-dihydroxyphenyl moiety, thus accounting for its higher binding affinity to XOD than allopurinol. These results indicate that A. confusa heartwood extracts and their major phytochemicals exhibit strong XOD inhibitory effects, which reduce serum uric acid levels while inhibiting uric acid generation in purine metabolism. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/20806936/Phytochemicals_from_Acacia_confusa_heartwood_extracts_reduce_serum_uric_acid_levels_in_oxonate_induced_mice:_their_potential_use_as_xanthine_oxidase_inhibitors_ L2 - https://dx.doi.org/10.1021/jf102689k DB - PRIME DP - Unbound Medicine ER -