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Arbuscular mycorrhizal fungi restore normal growth in a white poplar clone grown on heavy metal-contaminated soil, and this is associated with upregulation of foliar metallothionein and polyamine biosynthetic gene expression.
Ann Bot. 2010 Nov; 106(5):791-802.AB

Abstract

BACKGROUND AND AIMS

It is increasingly evident that plant tolerance to stress is improved by mycorrhiza. Thus, suitable plant-fungus combinations may also contribute to the success of phytoremediation of heavy metal (HM)-polluted soil. Metallothioneins (MTs) and polyamines (PAs) are implicated in the response to HM stress in several plant species, but whether the response is modulated by arbuscular mycorrhizal fungi (AMF) remains to be clarified. The aim of the present study was to check whether colonization by AMF could modify growth, metal uptake/translocation, and MT and PA gene expression levels in white poplar cuttings grown on HM-contaminated soil, and to compare this with plants grown on non-contaminated soil.

METHODS

In this greenhouse study, plants of a Populus alba clone were pre-inoculated, or not, with either Glomus mosseae or G. intraradices and then grown in pots containing either soil collected from a multimetal- (Cu and Zn) polluted site or non-polluted soil. The expression of MT and PA biosynthetic genes was analysed in leaves using quantitative reverse transcription-PCR. Free and conjugated foliar PA concentrations were determined in parallel.

RESULTS

On polluted soil, AMF restored plant biomass despite higher Cu and Zn accumulation in plant organs, especially roots. Inoculation with the AMF caused an overall induction of PaMT1, PaMT2, PaMT3, PaSPDS1, PaSPDS2 and PaADC gene expression, together with increased free and conjugated PA levels, in plants grown on polluted soil, but not in those grown on non-polluted soil.

CONCLUSIONS

Mycorrhizal plants of P. alba clone AL35 exhibit increased capacity for stabilization of soil HMs, together with improved growth. Their enhanced stress tolerance may derive from the transcriptional upregulation of several stress-related genes, and the protective role of PAs.

Authors+Show Affiliations

Dipartimento di Chimica, Università di Salerno, Stecca 7, Via Ponte don Melillo, 84084 Fisciano (SA), Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20810743

Citation

Cicatelli, Angela, et al. "Arbuscular Mycorrhizal Fungi Restore Normal Growth in a White Poplar Clone Grown On Heavy Metal-contaminated Soil, and This Is Associated With Upregulation of Foliar Metallothionein and Polyamine Biosynthetic Gene Expression." Annals of Botany, vol. 106, no. 5, 2010, pp. 791-802.
Cicatelli A, Lingua G, Todeschini V, et al. Arbuscular mycorrhizal fungi restore normal growth in a white poplar clone grown on heavy metal-contaminated soil, and this is associated with upregulation of foliar metallothionein and polyamine biosynthetic gene expression. Ann Bot. 2010;106(5):791-802.
Cicatelli, A., Lingua, G., Todeschini, V., Biondi, S., Torrigiani, P., & Castiglione, S. (2010). Arbuscular mycorrhizal fungi restore normal growth in a white poplar clone grown on heavy metal-contaminated soil, and this is associated with upregulation of foliar metallothionein and polyamine biosynthetic gene expression. Annals of Botany, 106(5), 791-802. https://doi.org/10.1093/aob/mcq170
Cicatelli A, et al. Arbuscular Mycorrhizal Fungi Restore Normal Growth in a White Poplar Clone Grown On Heavy Metal-contaminated Soil, and This Is Associated With Upregulation of Foliar Metallothionein and Polyamine Biosynthetic Gene Expression. Ann Bot. 2010;106(5):791-802. PubMed PMID: 20810743.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Arbuscular mycorrhizal fungi restore normal growth in a white poplar clone grown on heavy metal-contaminated soil, and this is associated with upregulation of foliar metallothionein and polyamine biosynthetic gene expression. AU - Cicatelli,Angela, AU - Lingua,Guido, AU - Todeschini,Valeria, AU - Biondi,Stefania, AU - Torrigiani,Patrizia, AU - Castiglione,Stefano, Y1 - 2010/09/01/ PY - 2010/9/3/entrez PY - 2010/9/3/pubmed PY - 2011/2/3/medline SP - 791 EP - 802 JF - Annals of botany JO - Ann Bot VL - 106 IS - 5 N2 - BACKGROUND AND AIMS: It is increasingly evident that plant tolerance to stress is improved by mycorrhiza. Thus, suitable plant-fungus combinations may also contribute to the success of phytoremediation of heavy metal (HM)-polluted soil. Metallothioneins (MTs) and polyamines (PAs) are implicated in the response to HM stress in several plant species, but whether the response is modulated by arbuscular mycorrhizal fungi (AMF) remains to be clarified. The aim of the present study was to check whether colonization by AMF could modify growth, metal uptake/translocation, and MT and PA gene expression levels in white poplar cuttings grown on HM-contaminated soil, and to compare this with plants grown on non-contaminated soil. METHODS: In this greenhouse study, plants of a Populus alba clone were pre-inoculated, or not, with either Glomus mosseae or G. intraradices and then grown in pots containing either soil collected from a multimetal- (Cu and Zn) polluted site or non-polluted soil. The expression of MT and PA biosynthetic genes was analysed in leaves using quantitative reverse transcription-PCR. Free and conjugated foliar PA concentrations were determined in parallel. RESULTS: On polluted soil, AMF restored plant biomass despite higher Cu and Zn accumulation in plant organs, especially roots. Inoculation with the AMF caused an overall induction of PaMT1, PaMT2, PaMT3, PaSPDS1, PaSPDS2 and PaADC gene expression, together with increased free and conjugated PA levels, in plants grown on polluted soil, but not in those grown on non-polluted soil. CONCLUSIONS: Mycorrhizal plants of P. alba clone AL35 exhibit increased capacity for stabilization of soil HMs, together with improved growth. Their enhanced stress tolerance may derive from the transcriptional upregulation of several stress-related genes, and the protective role of PAs. SN - 1095-8290 UR - https://www.unboundmedicine.com/medline/citation/20810743/Arbuscular_mycorrhizal_fungi_restore_normal_growth_in_a_white_poplar_clone_grown_on_heavy_metal_contaminated_soil_and_this_is_associated_with_upregulation_of_foliar_metallothionein_and_polyamine_biosynthetic_gene_expression_ L2 - https://academic.oup.com/aob/article-lookup/doi/10.1093/aob/mcq170 DB - PRIME DP - Unbound Medicine ER -