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Detection of early and single infections of Schistosoma japonicum in the intermediate host snail, Oncomelania hupensis, by PCR and loop-mediated isothermal amplification (LAMP) assay.
Am J Trop Med Hyg 2010; 83(3):542-8AJ

Abstract

Polymerase chain reaction (PCR) with the specific primer set amplifying 28S ribosomal DNA (rDNA) of Schistosoma japonicum was able to detect genomic DNA of S. japonicum, but not S. mansoni, at 100 fg. This procedure enabled us to detect the DNA from a single miracidium and a snail infected with one miracidium at just 1 day after infection. We compared these results with those from loop-mediated isothermal amplification (LAMP) targeting 28S rDNA and found similar results. The LAMP could amplify the specific DNA from a group of 100 normal snails mixed with one infected snail A PCR screening of infected snails from endemic regions in Anhui Province revealed schistosomal DNA even in snails found negative by microscopy. PCR and LAMP show promise for monitoring the early infection rate in snails, and they may be useful for predicting the risk of infection in the endemic places.

Authors+Show Affiliations

Section of Environmental Parasitology, Department of International Health Development, Division of Public Health, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, Tokyo, Japan. tkuma.vip@tmd.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20810818

Citation

Kumagai, Takashi, et al. "Detection of Early and Single Infections of Schistosoma Japonicum in the Intermediate Host Snail, Oncomelania Hupensis, By PCR and Loop-mediated Isothermal Amplification (LAMP) Assay." The American Journal of Tropical Medicine and Hygiene, vol. 83, no. 3, 2010, pp. 542-8.
Kumagai T, Furushima-Shimogawara R, Ohmae H, et al. Detection of early and single infections of Schistosoma japonicum in the intermediate host snail, Oncomelania hupensis, by PCR and loop-mediated isothermal amplification (LAMP) assay. Am J Trop Med Hyg. 2010;83(3):542-8.
Kumagai, T., Furushima-Shimogawara, R., Ohmae, H., Wang, T. P., Lu, S., Chen, R., ... Ohta, N. (2010). Detection of early and single infections of Schistosoma japonicum in the intermediate host snail, Oncomelania hupensis, by PCR and loop-mediated isothermal amplification (LAMP) assay. The American Journal of Tropical Medicine and Hygiene, 83(3), pp. 542-8. doi:10.4269/ajtmh.2010.10-0016.
Kumagai T, et al. Detection of Early and Single Infections of Schistosoma Japonicum in the Intermediate Host Snail, Oncomelania Hupensis, By PCR and Loop-mediated Isothermal Amplification (LAMP) Assay. Am J Trop Med Hyg. 2010;83(3):542-8. PubMed PMID: 20810818.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detection of early and single infections of Schistosoma japonicum in the intermediate host snail, Oncomelania hupensis, by PCR and loop-mediated isothermal amplification (LAMP) assay. AU - Kumagai,Takashi, AU - Furushima-Shimogawara,Rieko, AU - Ohmae,Hiroshi, AU - Wang,Tian-Ping, AU - Lu,Shaohong, AU - Chen,Rui, AU - Wen,Liyong, AU - Ohta,Nobuo, PY - 2010/9/3/entrez PY - 2010/9/3/pubmed PY - 2010/9/29/medline SP - 542 EP - 8 JF - The American journal of tropical medicine and hygiene JO - Am. J. Trop. Med. Hyg. VL - 83 IS - 3 N2 - Polymerase chain reaction (PCR) with the specific primer set amplifying 28S ribosomal DNA (rDNA) of Schistosoma japonicum was able to detect genomic DNA of S. japonicum, but not S. mansoni, at 100 fg. This procedure enabled us to detect the DNA from a single miracidium and a snail infected with one miracidium at just 1 day after infection. We compared these results with those from loop-mediated isothermal amplification (LAMP) targeting 28S rDNA and found similar results. The LAMP could amplify the specific DNA from a group of 100 normal snails mixed with one infected snail A PCR screening of infected snails from endemic regions in Anhui Province revealed schistosomal DNA even in snails found negative by microscopy. PCR and LAMP show promise for monitoring the early infection rate in snails, and they may be useful for predicting the risk of infection in the endemic places. SN - 1476-1645 UR - https://www.unboundmedicine.com/medline/citation/20810818/Detection_of_early_and_single_infections_of_Schistosoma_japonicum_in_the_intermediate_host_snail_Oncomelania_hupensis_by_PCR_and_loop_mediated_isothermal_amplification__LAMP__assay_ L2 - http://www.ajtmh.org/content/journals/10.4269/ajtmh.2010.10-0016?crawler=true&mimetype=application/pdf DB - PRIME DP - Unbound Medicine ER -