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NO2 decreases paracellular resistance to ion and solute flow in alveolar epithelial monolayers.
Exp Lung Res 1990 Nov-Dec; 16(6):561-75EL

Abstract

Primary cultured monolayers of rat alveolar epithelial cells grown on tissue culture-treated Nuclepore filters were exposed to 2.5 ppm nitrogen dioxide (NO2) for 2-20 min. Changes in monolayer bioelectric properties and solute permeabilities were subsequently measured. Exposure to NO2 produced a dose-dependent decrease in monolayer transepithelial electrical resistance (Rt), whereas monolayer short-circuit current was unaffected. Post-exposure monolayer permeability to 14C-sucrose (which primarily crosses alveolar epithelium via the paracellular pathway) increased markedly. That for 3H-glycerol (which permeates through both paracellular and transcellular pathways) increased to a lesser extent. Partial recovery of Rt and solute permeabilities was noted by 48-h post-exposure. The time courses of the decrease in Rt and increase in solute permeabilities were similar. These results suggest that NO2 primarily impairs passive alveolar epithelial barrier functions in vitro, probably by altering intercellular junctions, and does not appear to directly affect cell membrane active ion transport processes. When correlated with results obtained from experimental approaches, studies of in vitro alveolar epithelial monolayers may facilitate investigations of dosimetry, sites, and mechanisms of oxidant injury in the lung.

Authors+Show Affiliations

Seaver Cardiopulmonary Laboratory, Will Rogers Institute Pulmonary Research Program, Cornell University Medical College, New York, New York.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2081503

Citation

Cheek, J M., et al. "NO2 Decreases Paracellular Resistance to Ion and Solute Flow in Alveolar Epithelial Monolayers." Experimental Lung Research, vol. 16, no. 6, 1990, pp. 561-75.
Cheek JM, Kim KJ, Crandall ED. NO2 decreases paracellular resistance to ion and solute flow in alveolar epithelial monolayers. Exp Lung Res. 1990;16(6):561-75.
Cheek, J. M., Kim, K. J., & Crandall, E. D. (1990). NO2 decreases paracellular resistance to ion and solute flow in alveolar epithelial monolayers. Experimental Lung Research, 16(6), pp. 561-75.
Cheek JM, Kim KJ, Crandall ED. NO2 Decreases Paracellular Resistance to Ion and Solute Flow in Alveolar Epithelial Monolayers. Exp Lung Res. 1990;16(6):561-75. PubMed PMID: 2081503.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - NO2 decreases paracellular resistance to ion and solute flow in alveolar epithelial monolayers. AU - Cheek,J M, AU - Kim,K J, AU - Crandall,E D, PY - 1990/11/1/pubmed PY - 1990/11/1/medline PY - 1990/11/1/entrez SP - 561 EP - 75 JF - Experimental lung research JO - Exp. Lung Res. VL - 16 IS - 6 N2 - Primary cultured monolayers of rat alveolar epithelial cells grown on tissue culture-treated Nuclepore filters were exposed to 2.5 ppm nitrogen dioxide (NO2) for 2-20 min. Changes in monolayer bioelectric properties and solute permeabilities were subsequently measured. Exposure to NO2 produced a dose-dependent decrease in monolayer transepithelial electrical resistance (Rt), whereas monolayer short-circuit current was unaffected. Post-exposure monolayer permeability to 14C-sucrose (which primarily crosses alveolar epithelium via the paracellular pathway) increased markedly. That for 3H-glycerol (which permeates through both paracellular and transcellular pathways) increased to a lesser extent. Partial recovery of Rt and solute permeabilities was noted by 48-h post-exposure. The time courses of the decrease in Rt and increase in solute permeabilities were similar. These results suggest that NO2 primarily impairs passive alveolar epithelial barrier functions in vitro, probably by altering intercellular junctions, and does not appear to directly affect cell membrane active ion transport processes. When correlated with results obtained from experimental approaches, studies of in vitro alveolar epithelial monolayers may facilitate investigations of dosimetry, sites, and mechanisms of oxidant injury in the lung. SN - 0190-2148 UR - https://www.unboundmedicine.com/medline/citation/2081503/NO2_decreases_paracellular_resistance_to_ion_and_solute_flow_in_alveolar_epithelial_monolayers_ L2 - http://www.tandfonline.com/doi/full/10.3109/01902149009087880 DB - PRIME DP - Unbound Medicine ER -