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Carbon monoxide uptake kinetics of arterial, venous and capillary blood during CO rebreathing.
Exp Physiol. 2010 Dec; 95(12):1156-66.EP

Abstract

The uptake and distribution of CO throughout the circulatory system during two different methods of CO rebreathing (2 min 'Schmidt' and 40 min 'Burge' methods) were determined in nine healthy volunteers. Specifically, the impact of (i) differences in circulatory mixing time (t(mix)), (ii) CO diffusion to myoglobin (Mb) and (iii) CO wash-out was assessed on calculated haemoglobin mass (Hb(mass)). Arterial (a), muscle venous (vm) and capillary samples (c) were obtained simultaneously at 0, 1, 2, 3.5, 5, 7.5, 10, 12.5, 15, 20, 30 and 40 min for determination of carboxyhaemoglobin (HbCO). Carbon monoxide wash-out was measured from expired air following rebreathing. The rate of CO diffusion to Mb was calculated using the change in HbCO after t(mix), and the rate of CO wash-out. In both methods, HbCOa and HbCOc followed a near-identical time course, peaking within the first 2 min and decreasing thereafter. The HbCOvm increased slowly and was significantly lower at 1, 2 and 3.5 min in both methods (P < 0.01). The HbCOa peaked significantly higher in the Schmidt method (P = 0.03). Circulatory mixing had occurred by 10 min in most but not all subjects. The rate of CO wash-out was 0.25 ± 0.13 ml min⁻¹ in the Schmidt and 0.25 ± 0.16 ml min⁻¹ in the Burge method. The rate of CO diffusion to Mb was 0.22 ± 0.11 and 0.16 ± 0.13 ml min⁻¹ (P = 0.63) in Schmidt and Burge methods, respectively. Inhalation of a CO bolus during the Schmidt method results in faster HbCOa uptake but does not greatly shorten t(mix) or influence rates of CO wash-out and flux to Mb. The calculated Hb(mass) depends substantially on the plateau level of HbCO; therefore, it is paramount to ensure HbCO is mixed completely prior to blood sampling, as well as accounting for potential within-subject alterations of CO exhalation and CO flux to Mb.

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Authors+Show Affiliations

Garvican LA
Department of Physiology, Australian Institute of Sport, PO Box 176, Belconnen, ACT 2616, Australia. laura.garvican@ausport.gov.au
Burge CM
No affiliation info available
Cox AJ
No affiliation info available
Clark SA
No affiliation info available
Martin DT
No affiliation info available
Gore CJ
No affiliation info available

MeSH

AdultArteriesBlood Specimen CollectionCapillariesCarbon MonoxideCarboxyhemoglobinFemaleHumansMaleMiddle AgedRespirationVeins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

20817700

Citation

Garvican, Laura A., et al. "Carbon Monoxide Uptake Kinetics of Arterial, Venous and Capillary Blood During CO Rebreathing." Experimental Physiology, vol. 95, no. 12, 2010, pp. 1156-66.
Garvican LA, Burge CM, Cox AJ, et al. Carbon monoxide uptake kinetics of arterial, venous and capillary blood during CO rebreathing. Exp Physiol. 2010;95(12):1156-66.
Garvican, L. A., Burge, C. M., Cox, A. J., Clark, S. A., Martin, D. T., & Gore, C. J. (2010). Carbon monoxide uptake kinetics of arterial, venous and capillary blood during CO rebreathing. Experimental Physiology, 95(12), 1156-66. https://doi.org/10.1113/expphysiol.2010.054031
Garvican LA, et al. Carbon Monoxide Uptake Kinetics of Arterial, Venous and Capillary Blood During CO Rebreathing. Exp Physiol. 2010;95(12):1156-66. PubMed PMID: 20817700.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Carbon monoxide uptake kinetics of arterial, venous and capillary blood during CO rebreathing. AU - Garvican,Laura A, AU - Burge,Caroline M, AU - Cox,Amanda J, AU - Clark,Sally A, AU - Martin,David T, AU - Gore,Christopher J, Y1 - 2010/09/03/ PY - 2010/9/7/entrez PY - 2010/9/8/pubmed PY - 2011/3/19/medline SP - 1156 EP - 66 JF - Experimental physiology JO - Exp Physiol VL - 95 IS - 12 N2 - The uptake and distribution of CO throughout the circulatory system during two different methods of CO rebreathing (2 min 'Schmidt' and 40 min 'Burge' methods) were determined in nine healthy volunteers. Specifically, the impact of (i) differences in circulatory mixing time (t(mix)), (ii) CO diffusion to myoglobin (Mb) and (iii) CO wash-out was assessed on calculated haemoglobin mass (Hb(mass)). Arterial (a), muscle venous (vm) and capillary samples (c) were obtained simultaneously at 0, 1, 2, 3.5, 5, 7.5, 10, 12.5, 15, 20, 30 and 40 min for determination of carboxyhaemoglobin (HbCO). Carbon monoxide wash-out was measured from expired air following rebreathing. The rate of CO diffusion to Mb was calculated using the change in HbCO after t(mix), and the rate of CO wash-out. In both methods, HbCOa and HbCOc followed a near-identical time course, peaking within the first 2 min and decreasing thereafter. The HbCOvm increased slowly and was significantly lower at 1, 2 and 3.5 min in both methods (P < 0.01). The HbCOa peaked significantly higher in the Schmidt method (P = 0.03). Circulatory mixing had occurred by 10 min in most but not all subjects. The rate of CO wash-out was 0.25 ± 0.13 ml min⁻¹ in the Schmidt and 0.25 ± 0.16 ml min⁻¹ in the Burge method. The rate of CO diffusion to Mb was 0.22 ± 0.11 and 0.16 ± 0.13 ml min⁻¹ (P = 0.63) in Schmidt and Burge methods, respectively. Inhalation of a CO bolus during the Schmidt method results in faster HbCOa uptake but does not greatly shorten t(mix) or influence rates of CO wash-out and flux to Mb. The calculated Hb(mass) depends substantially on the plateau level of HbCO; therefore, it is paramount to ensure HbCO is mixed completely prior to blood sampling, as well as accounting for potential within-subject alterations of CO exhalation and CO flux to Mb. SN - 1469-445X UR - https://www.unboundmedicine.com/medline/citation/20817700/Carbon_monoxide_uptake_kinetics_of_arterial_venous_and_capillary_blood_during_CO_rebreathing_ L2 - https://doi.org/10.1113/expphysiol.2010.054031 DB - PRIME DP - Unbound Medicine ER -