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microRNA-1 and microRNA-206 regulate skeletal muscle satellite cell proliferation and differentiation by repressing Pax7.
J Cell Biol. 2010 Sep 06; 190(5):867-79.JC

Abstract

Skeletal muscle satellite cells are adult stem cells responsible for postnatal skeletal muscle growth and regeneration. Paired-box transcription factor Pax7 plays a central role in satellite cell survival, self-renewal, and proliferation. However, how Pax7 is regulated during the transition from proliferating satellite cells to differentiating myogenic progenitor cells is largely unknown. In this study, we find that miR-1 and miR-206 are sharply up-regulated during satellite cell differentiation and down-regulated after muscle injury. We show that miR-1 and miR-206 facilitate satellite cell differentiation by restricting their proliferative potential. We identify Pax7 as one of the direct regulatory targets of miR-1 and miR-206. Inhibition of miR-1 and miR-206 substantially enhances satellite cell proliferation and increases Pax7 protein level in vivo. Conversely, sustained Pax7 expression as a result of the loss of miR-1 and miR-206 repression elements at its 3' untranslated region significantly inhibits myoblast differentiation. Therefore, our experiments suggest that microRNAs participate in a regulatory circuit that allows rapid gene program transitions from proliferation to differentiation.

Authors+Show Affiliations

McAllister Heart Institute, Department of Cell and Developmental Biology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

20819939

Citation

Chen, Jian-Fu, et al. "MicroRNA-1 and microRNA-206 Regulate Skeletal Muscle Satellite Cell Proliferation and Differentiation By Repressing Pax7." The Journal of Cell Biology, vol. 190, no. 5, 2010, pp. 867-79.
Chen JF, Tao Y, Li J, et al. MicroRNA-1 and microRNA-206 regulate skeletal muscle satellite cell proliferation and differentiation by repressing Pax7. J Cell Biol. 2010;190(5):867-79.
Chen, J. F., Tao, Y., Li, J., Deng, Z., Yan, Z., Xiao, X., & Wang, D. Z. (2010). MicroRNA-1 and microRNA-206 regulate skeletal muscle satellite cell proliferation and differentiation by repressing Pax7. The Journal of Cell Biology, 190(5), 867-79. https://doi.org/10.1083/jcb.200911036
Chen JF, et al. MicroRNA-1 and microRNA-206 Regulate Skeletal Muscle Satellite Cell Proliferation and Differentiation By Repressing Pax7. J Cell Biol. 2010 Sep 6;190(5):867-79. PubMed PMID: 20819939.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - microRNA-1 and microRNA-206 regulate skeletal muscle satellite cell proliferation and differentiation by repressing Pax7. AU - Chen,Jian-Fu, AU - Tao,Yazhong, AU - Li,Juan, AU - Deng,Zhongliang, AU - Yan,Zhen, AU - Xiao,Xiao, AU - Wang,Da-Zhi, PY - 2010/9/8/entrez PY - 2010/9/8/pubmed PY - 2010/12/16/medline SP - 867 EP - 79 JF - The Journal of cell biology JO - J Cell Biol VL - 190 IS - 5 N2 - Skeletal muscle satellite cells are adult stem cells responsible for postnatal skeletal muscle growth and regeneration. Paired-box transcription factor Pax7 plays a central role in satellite cell survival, self-renewal, and proliferation. However, how Pax7 is regulated during the transition from proliferating satellite cells to differentiating myogenic progenitor cells is largely unknown. In this study, we find that miR-1 and miR-206 are sharply up-regulated during satellite cell differentiation and down-regulated after muscle injury. We show that miR-1 and miR-206 facilitate satellite cell differentiation by restricting their proliferative potential. We identify Pax7 as one of the direct regulatory targets of miR-1 and miR-206. Inhibition of miR-1 and miR-206 substantially enhances satellite cell proliferation and increases Pax7 protein level in vivo. Conversely, sustained Pax7 expression as a result of the loss of miR-1 and miR-206 repression elements at its 3' untranslated region significantly inhibits myoblast differentiation. Therefore, our experiments suggest that microRNAs participate in a regulatory circuit that allows rapid gene program transitions from proliferation to differentiation. SN - 1540-8140 UR - https://www.unboundmedicine.com/medline/citation/20819939/microRNA_1_and_microRNA_206_regulate_skeletal_muscle_satellite_cell_proliferation_and_differentiation_by_repressing_Pax7_ L2 - https://rupress.org/jcb/article-lookup/doi/10.1083/jcb.200911036 DB - PRIME DP - Unbound Medicine ER -